LOCUS       sequence-194960-        8473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PresentER with removable cassette for cloning of MHC-I antigen 
            (mCherry version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Gejman RS, Chang AY, Jones HF, DiKun K, Hakimi AA, Schietinger A, 
            Scheinberg DA
  TITLE     Rejection of immunogenic tumor clones is limited by clonal fraction.
  JOURNAL   Elife. 2018 Nov 30;7. pii: 41090. doi: 10.7554/eLife.41090.
  PUBMED    30499773
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-11-30"; volume: "7. pii: 41090. doi"; pages: " 
            10.7554/eLife.41090"
COMMENT     Sequence Label: PresentER-Cassette mCherry
FEATURES             Location/Qualifiers
     source          1..8473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1034..1052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1090..1112)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1212..1231
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1425..1447
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1575..1594
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             1794..2310
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2374..2715
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2782..3198
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3128..3150
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3166..3182
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           3238..3331
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3937..4049
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        4063..4562
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4098..4121)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4495..4514
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4583..5182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4583..4602)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5079..5099
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5242..5808
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5259..5810
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5403..5420)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5630..5649
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5822..6532
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5967..5985)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6235..6254
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6630..7144
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(7313..7329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7313..7329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7326..7348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7337..7353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7361..7367,7368..7385,7386..7391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7406..7427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7544..7561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7715..8303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7795..7814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1201 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     1321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1441 taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     1501 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     1561 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     1621 gtgatgccgg ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg
     1681 atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa gcctatagag
     1741 tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     1801 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     1861 atacataact gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata
     1921 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     1981 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
     2041 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     2101 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     2161 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc
     2221 tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg
     2281 attgactgcc cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct
     2341 gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct
     2401 gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag
     2461 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
     2521 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
     2581 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
     2641 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
     2701 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     2761 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     2821 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     2881 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     2941 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     3001 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     3061 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     3121 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     3181 actccttctc taggcgccgg aattagatct ctcgactagg gataacaggg taattgtttg
     3241 aatgaggctt cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga
     3301 ttacttcttc aggttaaccc aacagaaggc tcgaggtgac tggagttcag acgtgtgctc
     3361 ttccgatcgc caccatgcct aatcatcagt ccgggtcacc taccggcagt tcagacctgc
     3421 tccttgatgg caagaaacaa cgagcccatc tggcgctgag gagaaaacgg cgacgggaaa
     3481 tgcgcaagat taaccgaaag gtgagaagaa tgaatctcgc acccattaaa gaaaaaacag
     3541 cctggcagca cctgcaagct ctgatcttcg aggcggaaga agtgttgaag acttctcaaa
     3601 ctccacagac ctccctcacg ctgtttctag cactcttggc cgtattggcc acatttgctt
     3661 ctgacacaac tgtgttcact agcaacctca aacagacacc atggtgcatc tgactcctga
     3721 ggagaagtct gccgttactg ccctgtgggg caaggtgaac gtggatgaag ttggtggtga
     3781 ggccctgggc aggttggtat caaggttaca agacaggttt aaggagacca atagaaactg
     3841 ggcatgtgga gacagagaag ggccaaacag gccaaagatc ggaagagcgt cgtgtaggga
     3901 aagagtgtag atctcggtgg tcgccgtatc attgaattca aggggctact ttaggagcaa
     3961 ttatcttgtt tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg
     4021 aattaaaatg gtataaatta aatcactttt ttcaattcta ccgggtaggg gaggcgcttt
     4081 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4141 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4201 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4261 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4321 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4381 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4441 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4501 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4561 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4621 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4681 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4741 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4801 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4861 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4921 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4981 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5041 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5101 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5161 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     5221 cgcacgaccc catcatccaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     5281 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5341 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5401 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5461 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5521 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5581 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5641 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5701 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     5761 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     5821 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     5881 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     5941 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     6001 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     6061 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     6121 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     6181 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     6241 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     6301 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     6361 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     6421 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     6481 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aattaattaa
     6541 gaattatcaa gcttatcgat accgtcgacc tgcagccaag cttatcgata aaataaaaga
     6601 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     6661 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     6721 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     6781 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct
     6841 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     6901 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     6961 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac
     7021 tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     7081 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     7141 tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt
     7201 aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat
     7261 agttcattat ggacagcgca gaagagctgg ggagaattaa ttcgtaatca tggtcatagc
     7321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8461 cttggtctga cag
//
