LOCUS       sequence-19497-e         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF14
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    254..478
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagacccg gggagaagcc
      121 gcatatttgc cacatccaag gctgtgggaa ggtgtacggc gatcgtagcg cactggcacg
      181 tcacttgcgc tggcataccg gtggatcctc tgcacagtag taagcttaag tttaaaccgc
      241 tgatcagcct ccactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      301 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      361 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacatc
      421 aagggggagg attgggaaaa caatagcagg catgctgggg atgcggtggg ctctatggct
      481 tctgaagcgg aaagaac
//
