LOCUS       sequence-194978-        3390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Zarse K, Schulz TJ, Birringer M, Ristow M
  TITLE     Impaired respiration is positively correlated with decreased life 
            span in Caenorhabditis elegans models of Friedreich Ataxia.
  JOURNAL   FASEB J. 2007 Apr . 21(4):1271-5.
  PUBMED    17215485
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2007 Apr . 21(4):1271-5."
COMMENT     Sequence Label: frh-1-B
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        819..837
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1581..1597)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1582..1598)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1623..1641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1651..1668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1651..1667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1660..1682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1809..2264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1896..1915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2106..2127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2290..2394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2395..2463,2464..3255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2613..2632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc aacctggctt atcgaaatta atacgactca ctatagggag
      841 accggcagat ctgatgggtt agacatgtcg cgagaaatcg attcggtcat cggccagaat
      901 ctttcggaat tctctgttta gaagcgagtc aagttgttca ccatcgtgtg cataagtcca
      961 tctgaaatca aaaagcgtta atttgtgctc atcctcttcg cctcttttct gcactttacg
     1021 caataagaca cttgcatgtt actcatctcg gcttaatctc atttgtctgc aaactgatat
     1081 atatctaaat cttctctttt gatgttcgtg aaacctttcg ttacacgatc atcttgatca
     1141 tattcggtct agtggagttg tgcttttgat caagatatac aatttaaata atgaaaatgt
     1201 gtttttacta actttccctc ctcttctaaa tcatatcttt tcggaccaga catcggactg
     1261 gacaaccaaa tttgcttatt tggtgattgt ttgttaataa catacgttcc tgaaattaag
     1321 taattaattt aagaaataca agaaccatct cgtaccaaca gatttcgata cattaacagt
     1381 caaaactccc attgcatgag atacatcaaa ttgttcggaa accggaaatg aatcggcaat
     1441 ttgatcgaaa tagtctgata atctttctaa tgttgaatca gctgctgttt cgtactcatt
     1501 ttgactgaaa atccgggacg aaaatgagcg acgtacaaaa ttgtttcgta gaatagtgga
     1561 gagcatccca tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc
     1621 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     1681 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1741 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1801 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2041 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2221 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3361 atcccttaac gtgagttttc gttccactga
//
