LOCUS       sequence-194982-        8958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector containing SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-SaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      603..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             615..635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             660..3815
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             3816..3863
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3894..4043
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4068..4088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4518..4874
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4893..5462
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(5490..5507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5496..5703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5710..5958
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5710..5730
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5881..5900
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5979..6054
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     repeat_region   6068..6208
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6068..6197
                     /label=AAV2 ITR
     rep_origin      6283..6738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6370..6389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6580..6601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6755..6774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6874..6896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6934..6952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7020..7124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7125..7193,7194..7985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7343..7362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8156..8744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8645..8664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   8806..8935
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactaccg
      601 gtgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagcca
      661 agcggaacta catcctgggc ctggacatcg gcatcaccag cgtgggctac ggcatcatcg
      721 actacgagac acgggacgtg atcgatgccg gcgtgcggct gttcaaagag gccaacgtgg
      781 aaaacaacga gggcaggcgg agcaagagag gcgccagaag gctgaagcgg cggaggcggc
      841 atagaatcca gagagtgaag aagctgctgt tcgactacaa cctgctgacc gaccacagcg
      901 agctgagcgg catcaacccc tacgaggcca gagtgaaggg cctgagccag aagctgagcg
      961 aggaagagtt ctctgccgcc ctgctgcacc tggccaagag aagaggcgtg cacaacgtga
     1021 acgaggtgga agaggacacc ggcaacgagc tgtccaccaa agagcagatc agccggaaca
     1081 gcaaggccct ggaagagaaa tacgtggccg aactgcagct ggaacggctg aagaaagacg
     1141 gcgaagtgcg gggcagcatc aacagattca agaccagcga ctacgtgaaa gaagccaaac
     1201 agctgctgaa ggtgcagaag gcctaccacc agctggacca gagcttcatc gacacctaca
     1261 tcgacctgct ggaaacccgg cggacctact atgagggacc tggcgagggc agccccttcg
     1321 gctggaagga catcaaagaa tggtacgaga tgctgatggg ccactgcacc tacttccccg
     1381 aggaactgcg gagcgtgaag tacgcctaca acgccgacct gtacaacgcc ctgaacgacc
     1441 tgaacaatct cgtgatcacc agggacgaga acgagaagct ggaatattac gagaagttcc
     1501 agatcatcga gaacgtgttc aagcagaaga agaagcccac cctgaagcag atcgccaaag
     1561 aaatcctcgt gaacgaagag gatattaagg gctacagagt gaccagcacc ggcaagcccg
     1621 agttcaccaa cctgaaggtg taccacgaca tcaaggacat taccgcccgg aaagagatta
     1681 ttgagaacgc cgagctgctg gatcagattg ccaagatcct gaccatctac cagagcagcg
     1741 aggacatcca ggaagaactg accaatctga actccgagct gacccaggaa gagatcgagc
     1801 agatctctaa tctgaagggc tataccggca cccacaacct gagcctgaag gccatcaacc
     1861 tgatcctgga cgagctgtgg cacaccaacg acaaccagat cgctatcttc aaccggctga
     1921 agctggtgcc caagaaggtg gacctgtccc agcagaaaga gatccccacc accctggtgg
     1981 acgacttcat cctgagcccc gtcgtgaaga gaagcttcat ccagagcatc aaagtgatca
     2041 acgccatcat caagaagtac ggcctgccca acgacatcat tatcgagctg gcccgcgaga
     2101 agaactccaa ggacgcccag aaaatgatca acgagatgca gaagcggaac cggcagacca
     2161 acgagcggat cgaggaaatc atccggacca ccggcaaaga gaacgccaag tacctgatcg
     2221 agaagatcaa gctgcacgac atgcaggaag gcaagtgcct gtacagcctg gaagccatcc
     2281 ctctggaaga tctgctgaac aaccccttca actatgaggt ggaccacatc atccccagaa
     2341 gcgtgtcctt cgacaacagc ttcaacaaca aggtgctcgt gaagcaggaa gaaaacagca
     2401 agaagggcaa ccggacccca ttccagtacc tgagcagcag cgacagcaag atcagctacg
     2461 aaaccttcaa gaagcacatc ctgaatctgg ccaagggcaa gggcagaatc agcaagacca
     2521 agaaagagta tctgctggaa gaacgggaca tcaacaggtt ctccgtgcag aaagacttca
     2581 tcaaccggaa cctggtggat accagatacg ccaccagagg cctgatgaac ctgctgcgga
     2641 gctacttcag agtgaacaac ctggacgtga aagtgaagtc catcaatggc ggcttcacca
     2701 gctttctgcg gcggaagtgg aagtttaaga aagagcggaa caaggggtac aagcaccacg
     2761 ccgaggacgc cctgatcatt gccaacgccg atttcatctt caaagagtgg aagaaactgg
     2821 acaaggccaa aaaagtgatg gaaaaccaga tgttcgagga aaagcaggcc gagagcatgc
     2881 ccgagatcga aaccgagcag gagtacaaag agatcttcat caccccccac cagatcaagc
     2941 acattaagga cttcaaggac tacaagtaca gccaccgggt ggacaagaag cctaatagag
     3001 agctgattaa cgacaccctg tactccaccc ggaaggacga caagggcaac accctgatcg
     3061 tgaacaatct gaacggcctg tacgacaagg acaatgacaa gctgaaaaag ctgatcaaca
     3121 agagccccga aaagctgctg atgtaccacc acgaccccca gacctaccag aaactgaagc
     3181 tgattatgga acagtacggc gacgagaaga atcccctgta caagtactac gaggaaaccg
     3241 ggaactacct gaccaagtac tccaaaaagg acaacggccc cgtgatcaag aagattaagt
     3301 attacggcaa caaactgaac gcccatctgg acatcaccga cgactacccc aacagcagaa
     3361 acaaggtcgt gaagctgtcc ctgaagccct acagattcga cgtgtacctg gacaatggcg
     3421 tgtacaagtt cgtgaccgtg aagaatctgg atgtgatcaa aaaagaaaac tactacgaag
     3481 tgaatagcaa gtgctatgag gaagctaaga agctgaagaa gatcagcaac caggccgagt
     3541 ttatcgcctc cttctacaac aacgatctga tcaagatcaa cggcgagctg tatagagtga
     3601 tcggcgtgaa caacgacctg ctgaaccgga tcgaagtgaa catgatcgac atcacctacc
     3661 gcgagtacct ggaaaacatg aacgacaaga ggccccccag gatcattaag acaatcgcct
     3721 ccaagaccca gagcattaag aagtacagca cagacattct gggcaacctg tatgaagtga
     3781 aatctaagaa gcaccctcag atcatcaaaa agggcaaaag gccggcggcc acgaaaaagg
     3841 ccggccaggc aaaaaagaaa aagggatccg aggccagcgg ttccggacgg gctgacgcat
     3901 tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca
     3961 tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg
     4021 atgatttcga cctggacatg ctgattaact ctagaagttc cggatctccg aaaaagaaac
     4081 gcaaagttgg tagccagtac ctgcccgaca ccgacgaccg gcaccggatc gaggaaaagc
     4141 ggaagcggac ctacgagaca ttcaagagca tcatgaagaa gtcccccttc agcggcccca
     4201 ccgaccctag gcctccacct agaagaatcg ccgtgcccag cagatccagc gccagcgtgc
     4261 caaaacctgc cccccagcct taccccttca ccagcagcct gagcaccatc aactacgacg
     4321 agttccctac catggtgttc cccagcggcc agatctctca ggcctctgct ctggccccag
     4381 cccctcctca ggtgctgcct caggctcctg ctcctgcacc agctccagcc atggtgtctg
     4441 cactggctca ggcaccagca cccgtgcctg tgctggctcc tggacctcca caggctgtgg
     4501 ctccaccagc ccctaaacct acacaggccg gcgagggcac actgtctgaa gctctgctgc
     4561 agctgcagtt cgacgacgag gatctgggag ccctgctggg aaacagcacc gatcctgccg
     4621 tgttcaccga cctggccagc gtggacaaca gcgagttcca gcagctgctg aaccagggca
     4681 tccctgtggc ccctcacacc accgagccca tgctgatgga ataccccgag gccatcaccc
     4741 ggctcgtgac aggcgctcag aggcctcctg atccagctcc tgcccctctg ggagcaccag
     4801 gcctgcctaa tggactgctg tctggcgacg aggacttcag ctctatcgcc gatatggatt
     4861 tctcagcctt gctgggctct ggcagcggca gccgggattc cagggaaggg atgtttttgc
     4921 cgaagcctga ggccggctcc gctattagtg acgtgtttga gggccgcgag gtgtgccagc
     4981 caaaacgaat ccggccattt catcctccag gaagtccatg ggccaaccgc ccactccccg
     5041 ccagcctcgc accaacacca accggtccag tacatgagcc agtcgggtca ctgaccccgg
     5101 caccagtccc tcagccactg gatccagcgc ccgcagtgac tcccgaggcc agtcacctgt
     5161 tggaggatcc cgatgaagag acgagccagg ctgtcaaagc ccttcgggag atggccgata
     5221 ctgtgattcc ccagaaggaa gaggctgcaa tctgtggcca aatggacctt tcccatccgc
     5281 ccccaagggg ccatctggat gagctgacaa ccacacttga gtccatgacc gaggatctga
     5341 acctggactc acccctgacc ccggaattga acgagattct ggataccttc ctgaacgacg
     5401 agtgcctctt gcatgccatg catatcagca caggactgtc catcttcgac acatctctgt
     5461 tttgagaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5521 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5581 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5641 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg
     5701 ggaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     5761 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     5821 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     5881 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     5941 tggaaaggac gaaacaccgg agaccacggc aggtctcagt tttagtactc tggaaacaga
     6001 atctactaaa acaaggcaaa atgccgtgtt tatctcgtca acttgttggc gagatttttg
     6061 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     6121 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     6181 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     6241 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     6301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     6361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6481 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6601 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     6661 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6721 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6781 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6841 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6901 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag
     7861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc
     8821 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca
     8881 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
     8941 ctctagactc gaggcgtt
//
