LOCUS       sequence-194983-       11315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HOXA5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-HOXA5
FEATURES             Location/Qualifiers
     source          1..11315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..39
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     36..60
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1013..1037)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1090..1116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1336..2514
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1567..2505
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2462..2482
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2584..2617
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2624..3241,3242..3247,3248..3283,3284..3286,3287..3322,
                     3323..3325,3326..3361,3362..3370)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3422..3995
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3589..3606)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3816..3835
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4011..4013,4014..4016,4017..4730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4011..4013,4014..4016,4017..4727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4056..4077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4317..4336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4664..4685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4866..4885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5173..5761
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5226..5246)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5644..5655)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5836..6069
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6188..6206)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6274..6378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6379..6447,6448..7239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6597..6616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7410..7998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7899..7918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8152..8169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8409..8430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8445..8450,8451..8468,8469..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8488..8510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8507..8523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8507..8523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8573..8592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8784..9417
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9464..9589
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10086..10319
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10504..10548
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10841..10958
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(11013..11031,11032..11054,11055..11073,11074..11096,
                     11097..11115,11116..11138,11139..11157,11158..11180,
                     11181..11199,11200..11222,11223..11241,11242..11264,
                     11265..11283)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
       61 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      121 ctccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
      181 aaaaaagcag gctccaccat gagctcttat tttgtaaact cattttgcgg tcgctatcca
      241 aatggcccgg actaccagtt gcataattat ggagatcata gttccgtgag cgagcaattc
      301 agggactcgg cgagcatgca ctccggcagg tacggctacg gctacaatgg catggatctc
      361 agcgtcggcc gctcgggctc cggccacttt ggctccggag agcgcgcccg cagctacgct
      421 gccagcgcca gcgcggcgcc cgccgagccc aggtacagcc agccggccac gtccacgcac
      481 tctcctcagc ccgatccgct gccctgctcc gccgtggccc cctcgcccgg cagcgacagc
      541 caccacggcg ggaaaaactc cctaagcaac tccagcggcg cctcggccga cgccggcagc
      601 acccacatca gcagcagaga gggggttggc acggcgtccg gagccgagga ggacgcccct
      661 gccagcagcg agcaggcgag tgcgcagagc gagccgagcc cggcgccgcc cgcccaaccc
      721 cagatctacc cctggatgcg caagctgcac ataagtcatg acaacatagg cggcccggaa
      781 ggcaaaaggg cccggacggc ctacacgcgc taccagaccc tggagctgga gaaggagttc
      841 cacttcaacc gttacctgac ccgcagaagg aggattgaaa tagcacatgc tctttgcctc
      901 tccgagagac aaattaaaat ctggttccaa aaccggagaa tgaagtggaa aaaagataat
      961 aagctgaaaa gcatgagcat ggccgcggca ggaggggcct tccgtcccta ggacccagct
     1021 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     1081 cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa
     1141 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc
     1201 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagccgc
     1261 gtaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     1321 cgagcctagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     1381 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     1441 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     1501 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     1561 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     1621 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     1681 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     1741 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     1801 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     1861 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     1921 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1981 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     2041 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     2101 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     2161 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     2221 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     2281 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     2341 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     2401 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     2461 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagcggc
     2521 cgccatgcta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgga
     2581 tccataactt cgtataatgt atgctatacg aagttatgcc accatgtcta gactggacaa
     2641 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     2701 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     2761 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     2821 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     2881 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     2941 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     3001 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     3061 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     3121 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     3181 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     3241 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     3301 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     3361 ccccgggtaa ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc
     3421 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     3481 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3541 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     3601 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     3661 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     3721 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3781 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3841 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3901 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     3961 gggacgtggt tttcctttga aaaacacgat gataagctta ccggtccacc atggtgagca
     4021 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4081 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4141 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4201 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4261 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4321 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4381 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4441 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4501 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4561 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4621 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4681 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gcatgcccga
     4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4981 tgacgagttc ttctgatgta caagtaaagc ggccgcgact ctagatcata atcagccata
     5041 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5101 aacataaaat gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc
     5161 gcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5221 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5281 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5341 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5401 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5461 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5521 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5581 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5641 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5701 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5761 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5821 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5881 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5941 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6001 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6061 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6121 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     6181 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6241 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6301 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6361 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6421 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6481 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6541 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6601 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6661 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6721 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6781 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6841 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6901 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6961 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7021 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7081 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7141 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7201 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8161 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8221 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
     8281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8341 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8521 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8581 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     8641 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     8701 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8761 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     8821 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     8881 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8941 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     9001 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     9061 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     9121 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     9181 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     9241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     9421 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9481 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     9541 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     9601 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     9661 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9721 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9781 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9841 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9901 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     9961 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    10021 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    10081 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    10141 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    10201 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10261 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10321 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10381 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10441 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10501 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10561 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10621 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10681 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10741 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    10801 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10861 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10921 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10981 gagatccagt ttggactagg atcctttacc actccctatc agtgatagag aaaagtgaaa
    11041 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
    11101 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
    11161 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
    11221 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
    11281 agaaaagtga aagtcgagct cggtacccgg gtcga
//
