LOCUS       sequence-194992-        8190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of DYRK1B fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Dong C, West KL, Tan XY, Li J, Ishibashi T, Yu CH, Sy SMH, Leung 
            JWC, Huen MSY
  TITLE     Screen identifies DYRK1B network as mediator of transcription 
            repression on damaged chromatin.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17019-17030. doi: 
            10.1073/pnas.2002193117. Epub 2020 Jul 1.
  PUBMED    32611815
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2002193117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-07-21- 21"; volume: "117"; issue: "29"; pages: 
            "17019-17030"
COMMENT     Sequence Label: pMH-SFB-DYRK1B
FEATURES             Location/Qualifiers
     source          1..8190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(274..291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(445..1033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(525..544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1193..1984,1985..2053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1816..1835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2054..2158)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2189..2658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2723..2922
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3123..3539
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3469..3491
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3507..3523
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        3617..3920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3921..4124
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4075..4095
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4184..4228
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4262..4285
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4289..4312
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4322..4435
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    4508..4532
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6420..6444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6626..6643)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(6705..6725)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6710..7039
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6890..7025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7049..7648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7049..7068)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7545..7565
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
       61 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
      121 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
      181 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
      241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1141 tttgcggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1501 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1801 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2161 catttccccg aaaagtgcca cctgcagcct gaatatgggc caaacaggat atctgtggta
     2221 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     2281 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     2341 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     2401 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     2461 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     2521 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     2581 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     2641 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     2701 caccgaccca ccaccgggag gcaagctggc cagcaactta tctgtgtctg tccgattgtc
     2761 tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta
     2821 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
     2881 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
     2941 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
     3001 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
     3061 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
     3121 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     3181 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     3241 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     3301 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     3361 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     3421 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     3481 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
     3541 gccggatccc agtgtggtgg tactaatagt aatcaattac ggggtcatta gttcatagcc
     3601 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3661 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3721 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3781 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3841 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     3901 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     3961 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4021 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4081 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     4141 agatccgcta gcgctaccgg actcagatct cgaggccacc atgaaagaaa ccgctgctgc
     4201 taaattcgaa cgccagcaca tggacagcgg agccggcgca ggagccggcg ctgacgcgcc
     4261 tgactacaaa gacgatgacg acaagggaga ttacaaggat gacgatgaca aaggcgcgtc
     4321 gatggacgag aagaccaccg gctggcgggg cggccacgtg gtggagggcc tggccggcga
     4381 gctggagcag ctgcgggcca ggctggagca ccaccctcag ggccagcggg agccctccgg
     4441 cggctgcaag ctgggcggcg cgcctgaatt ctgcagtcga cggtaccgcg ggcccatcgc
     4501 tcgagagaca agtttgtaca aaaaagttgg catggccgtc ccaccgggcc atggtccctt
     4561 ctctggcttc ccagggcccc aggagcacac gcaggtattg cctgatgtgc ggctactgcc
     4621 tcggaggctg cccctggcct tccgggatgc aacctcagcc ccgctgcgta agctctctgt
     4681 ggacctcatc aagacctaca agcacatcaa tgaggtatac tatgcgaaga agaagcggcg
     4741 ggcccagcag gcgccacccc aggattcgag caacaagaag gagaagaagg tcctgaacca
     4801 tggttatgat gacgacaacc atgactacat cgtgcgcagt ggcgagcgct ggctggagcg
     4861 ctacgaaatt gactcgctca ttggcaaagg ctcctttggc caggtggtga aagcctatga
     4921 tcatcagacc caggagcttg tggccatcaa gatcatcaag aacaaaaagg ctttcctgaa
     4981 ccaggcccag attgagctgc ggctgctgga gctgatgaac cagcatgaca cggagatgaa
     5041 gtactatata gtacacctga agcggcactt catgttccgg aaccacctgt gcctggtatt
     5101 tgagctgctg tcctacaacc tgtacgacct cctgcgcaac acccacttcc gcggcgtctc
     5161 gctgaacctg acccggaagc tggcgcagca gctctgcacg gcactgctct ttctggccac
     5221 gcctgagctc agcatcattc actgcgacct caagcccgaa aacatcttgc tgtgcaaccc
     5281 caagcgcagc gccatcaaga ttgtggactt cggcagctcc tgccagcttg gccagaggat
     5341 ctaccagtat atccagagcc gcttctaccg ctcacctgag gtgctcctgg gcacacccta
     5401 cgacctggcc attgacatgt ggtccctggg ctgcatcctt gtggagatgc acaccggaga
     5461 gcccctcttc agtggctcca atgaggtcga ccagatgaac cgcattgtgg aggtgctggg
     5521 catcccaccg gccgccatgc tggaccaggc gcccaaggct cgcaagtact ttgaacggct
     5581 gcctgggggt ggctggaccc tacgaaggac gaaagaactc aggaaggatt accagggccc
     5641 cgggacacgg cggctgcagg aggtgctggg cgtgcagacg ggcgggcccg ggggccggcg
     5701 ggcgggggag ccgggccaca gccccgccga ctacctccgc ttccaggacc tggtgctgcg
     5761 catgctggag tatgagcccg ccgcccgcat cagccccctg ggggctctgc agcacggctt
     5821 cttccgccgc acggccgacg aggccaccaa cacgggcccg gcaggcagca gtgcctccac
     5881 ctcgcccgcg cccctcgaca cctgcccctc ttccagcacc gccagctcca tctccagttc
     5941 tggaggctcc agtggctcct ccagtgacaa ccggacctac cgctacagca accgatattg
     6001 tgggggccct gggcccccta tcacagactg tgagatgaac agcccccagg tcccaccctc
     6061 ccagccgctg cggccctggg cagggggtga tgtgccccac aagacacatc aagcccctgc
     6121 ctctgcctcg tcactgcctg ggaccggggc ccagttaccc ccccagcccc gataccttgg
     6181 tcgtccccca tcaccaacct caccaccacc cccggagctg atggatgtga gcctggtggg
     6241 cggccctgct gactgctccc cacctcaccc agcgcctgcc ccccagcacc cggctgcctc
     6301 agccctccgg actcggatga ctggaggtcg tccacccctc ccgcctcctg atgaccctgc
     6361 cactctgggg cctcacctgg gcctccgtgg tgtaccccag agcacagcag ccagctcgtg
     6421 cccaactttc ttgtacaaag tggctcgagc agggatccgc ccctctccct ccccccccct
     6481 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6541 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6601 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6661 gtgaaggaag cgtaggaatt cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt
     6721 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6781 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6841 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6901 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6961 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7021 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     7081 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7141 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7201 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7261 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7321 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7381 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7441 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7501 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7561 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7621 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     7681 aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa
     7741 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     7801 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     7861 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     7921 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     7981 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     8041 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     8101 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     8161 agctcaataa aagagcccac aacccctcac
//
