LOCUS       sequence-195011-        2260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a DNA helicase, a chimeric construct of Bacilllus 
            stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2260)
  TITLE     Webb Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET22B_PcrA_2Bchimera
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gaactttctg agcgaacagc
       61 tgctggcaca tctgaataaa gaacagcaag aagcagttcg taccaccgaa ggtccgctgc
      121 tgattatggc aggcgcaggt agcggtaaaa cccgtgttct gacccatcgt attgcatatc
      181 tgatggcaga aaaacatgtt gcaccgtgga atattctggc aattaccttt accaataaag
      241 ccgcacgtga aatgcgtgaa cgtgttcaga gcctgctggg tggtgcagca gaagatgttt
      301 ggattagcac ctttcatagc atgtgtgttc gtattctgcg tcgtgatatt gatcgcattg
      361 gtattaatcg caactttagc attctggacc cgaccgatca gctgagcgtt atgaaaacca
      421 ttctgaaaga aaaaaacatc gatccgaaaa aattcgagcc tcgtaccatt ctgggcacca
      481 ttagcgcagc aaaaaatgaa ctgctgcctc cggaacagtt tgcaaaacgt gcaagcacct
      541 attatgaaaa agttgtgtcc gatgtgtacc aagaatatca gcaacgtctg ctgcgtaatc
      601 atagcctgga ttttgatgat ctgattatga ccaccatcca gctgtttgat cgtgttccgg
      661 atgttctgca ttattatcag tacaaattcc agtacatcca catcgatgaa tatcaggata
      721 ccaatcgtgc acagtatacc ctggttaaaa aactggcaga acgctttcag aacatttgtg
      781 cagttggtga tgcagatcag agcatttatc gttggcgtgg tgcagatatt caaaacattc
      841 tgagctttga acgcgattat ccgaatgcca aagttattct gctggaacag aattatcgta
      901 gcaccaaacg cattctgcag gcagcaaatg aagtgattga acataatgtg aaccgtaaac
      961 cgaaacgtct gtggaccgaa aatccggaag gtaaaccgat tctgtattat gaagcaatga
     1021 acgaagcaga tgaagcacag tttgttgcag gtcgtattcg tgaagcagtt gaacgtggtg
     1081 aacgtcgtta tcgtgatttt gcagttctgt atcgtaccaa tgcacagagc cgtgttatgg
     1141 aagaaatgct gctgaaagca aatatcccgt atcagattgt tggtggccag aaattctatg
     1201 atcgcaaaga aattaaagat ctgctgagct atctgcgcat tattgccaat agcaatgatg
     1261 atattagcct gcagcgtatt atcaatgttc cgaaacgtgg tgttggtccg agcagcgttg
     1321 aaaaagtgca gaattatgcc ctgcagaaca acatcagcat gtttgatgca ctgggtgaag
     1381 cagatttcat tggtctgagc aaaaaagtta cccaagaatg cctgaacttc tatgaactga
     1441 ttcagtccct gatcaaagaa caagaattcc tggaaatcca tgagatcgtt gatgaagtgc
     1501 tgcagaaaag cggttatcgc gaaatgctgg aacgtgaaaa taccctggaa agccgtagcc
     1561 gtctggaaaa tattgatgaa tttatgagcg tgccgaaaga ttatgaagaa aatacaccgc
     1621 tggaagaaca gagcctgatc aattttctga ccgatctgag cctggttgca gatatcgatg
     1681 aagcaaatgg caccgaacag gcagccgaag gtgatgccgt tatgctgatg accctgcatg
     1741 ccgcaaaagg tctggaattt ccggttgtgt ttctgattgg catggaagaa ggtatttttc
     1801 cgcataatcg ttccctggaa gatgatgatg aaatggaaga ggaacgtcgt ctggcctatg
     1861 ttggtattac ccgtgcagaa gaagaactgg ttctgaccag cgcacagatg cgtaccctgt
     1921 ttggtaacat tcagatgaat ccgcctagcc gttttctgaa tgaaattcct gcacatctgc
     1981 tggaaaccgc aagccgtcgt caggctggtg ccagccgtcc ggcagttagt cgtccgcagg
     2041 caagcggtgc cgttggtagc tggaaagtgg gtgatcgtgc aaatcatcgt aaatggggta
     2101 ttggcaccgt tgttagcgtt cgtggtggcg gtgatgatca agaactggat attgcatttc
     2161 cgagcccgat tggcattaaa cgcctgctgg caaaatttgc accgatcgaa aaagtgtaag
     2221 gatccgaatt cgagctccgt cgacaagctt gcggccgcac
//
