LOCUS       sequence-195038-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(153..170)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(153..169)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(162..184)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttaaggcct gagctcgaat tcactggccg tcgttttaca acgtcgtgac
      181 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      241 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      301 ggcgaatggc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgacggc
      361 aatagttacc cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct
      421 ttaaaaaaac acaaaagacc acatttttta atgtggtctt ttattcttca actaaagcac
      481 ccattagttc aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg
      541 ttacagtaat attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag
      601 cctcctaaat tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat
      661 tgatttagac aattggaaga gaaaagagat atttaatcat tatttgaa
//
