LOCUS       sequence-195084-        6505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histone fused jRGECO1a under the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou
            S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
  TITLE     Wide-area all-optical neurophysiology in acute brain slices.
  JOURNAL   J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019.
  PUBMED    30952812
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 
            10.1523/JNEUROSCI.0168-19.2019."
COMMENT     Sequence Label: pAAV-synp-F-H2B-jRGECO1a
FEATURES             Location/Qualifiers
     source          1..6505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        218..665
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(703..736)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(787..820)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             834..1211
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1224..1233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1245..1301
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1997..2016
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    2490..2523
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2574..2607
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2632..3220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2685..2705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3103..3114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3252..3728
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3451..3470)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3768..3908
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3768..3897
                     /label=AAV2 ITR
     rep_origin      3983..4438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4070..4089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4280..4301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4455..4474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4574..4596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4634..4652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4720..4824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4825..4893,4894..5685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5043..5062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5856..6444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6345..6364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgctcgacat tgattattga ctagtttaat
      181 taataccgcc atgcatctgc agagggccct gcgtatgagt gcaagtgggt tttaggacca
      241 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc
      301 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc
      361 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg
      421 gcggcgcgcg ccaccgccgc ctcatcactg aaggcgcgct gacgtcactc gccggtcccc
      481 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac
      541 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg
      601 gcgactcatc gctgcctcag tctgcggtgg gcagcggaag agtcgtgtcg tgcctgagag
      661 cgcagtcaag aaggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata
      721 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
      781 ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccgc accatgccag
      841 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
      901 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
      961 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
     1021 tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
     1081 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
     1141 tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca
     1201 ccagcgctaa ggatccaccg gtcgccacca tggtcgactc atcacgtcgt aagtggaata
     1261 aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt tccgagcgga
     1321 tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg ctgaaggacg
     1381 gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1441 ccggcgccta catcgtggac atcaagttgg acatcgtgtc ccacaacgag gactacacca
     1501 tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1561 acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac atggccatca
     1621 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     1681 agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct aagctgaagg
     1741 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     1801 gctccaaggc ctacattaag cacccagccg acatccccga ctacttcaag ctgtccttcc
     1861 ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt attcacgtga
     1921 accaggactc ctccctgcag gacggcgtat tcatctacaa ggtgaagctg cgcggcacca
     1981 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gctacgcgtg
     2041 acgacctgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta tttgacaagg
     2101 acggggatgg gacgataaca accaaggagc ttgggacggt gttccgatcg ctggggcaga
     2161 accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac ggtgacggca
     2221 cattcgactt ccctgagttc ctgacgatga tggcaagaaa aatgaatgac acagacagtg
     2281 aagaggaaat tcgcgaagcg ttccgcgtgt ttgataagga cggcaatggc tacatcggcg
     2341 cagcagagct tcgccacgtg atgacagacc ttggagagaa gttaacagat gaggaggttg
     2401 atgaaatgat cagggtagca gacatcgatg gggatggtca ggtaaactac gaagagtttg
     2461 tacaaatgat gacagcgaag taggctagca taacttcgta taaagtatcc tatacgaagt
     2521 tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa agaataactt
     2581 cgtataatgt atgctatacg aagttatgaa ttcgatatca agcttatcga taatcaacct
     2641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3001 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt
     3061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3181 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc
     3241 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     3301 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     3361 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     3421 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     3481 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     3541 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     3601 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     3661 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     3721 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     3781 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3841 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3901 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3961 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4321 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4381 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     4441 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4501 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4561 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4621 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4681 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6001 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6481 ttttgctggc cttttgctca catgt
//
