LOCUS       sequence-195134-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Joberty G, Petersen C, Gao L, Macara IG
  TITLE     The cell-polarity protein Par6 links Par3 and atypical protein 
            kinase C to Cdc42.
  JOURNAL   Nat Cell Biol 2000 Aug;2(8):531-9.
  PUBMED    10934474
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol"; date: "2000-08"; volume: "2(8)"; pages: "531-9"
COMMENT     Sequence Label: pK-myc-Par3b
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     162..184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        226..605
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        606..809
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     760..780
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     806..830
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1040..1058
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1040..1057
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1071..1090
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1145..1174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      5293..5302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5306..5440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5343..5362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5397..5416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5509..5866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5509..5700
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5532..5552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5717..5852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5886..5903)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5886..5902)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5895..5917)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6115..6570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6202..6221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6412..6433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6587..6606)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6706..6728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6766..6784)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6852..6956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6957..7025,7026..7817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7175..7194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7988..8576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8477..8496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8730..8747
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgacatg attacgaatt aattcgagct cgcccgacat tgattattga
      241 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      301 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      361 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      421 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      481 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      541 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      601 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      661 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      721 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      781 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      841 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      901 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
      961 gagtctatag gcccaccccc ttggcttcgt tagaacgcgg ctacaattaa tacataacct
     1021 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct
     1081 ctccacaggt gtccactccc aggtccaact gcacctcggt tctaagcttc tgcaggtcga
     1141 catggaacag aaactcatct ctgaagagga tctgtctaga ggatcccggg ctagcgggcg
     1201 gccgcatatg aaagtgaccg tgtgcttcgg acggacccgg gtggtcgtgc cgtgcgggga
     1261 cggccacatg aaagttttca gcctcatcca gcaggcggtg acccgctacc ggaaggccat
     1321 cgccaaggat ccaaactact ggatacaggt gcatcgcttg gaacatggag atggaggaat
     1381 actagacctt gatgacattc tttgtgatgt agcagacgat aaagacagac tggtagcagt
     1441 gtttgatgag caggatccac atcacggagg tgatggcacc agtgccagtt ccacgggtac
     1501 ccagagccca gagatatttg gtagtgagct tggcaccaac aatgtctcag cctttcagcc
     1561 ttaccaagca acaagtgaaa ttgaggtcac accttcagtc cttcgagcaa atatgcctct
     1621 tcatgttcga cgcagtagtg acccagctct aattggcctc tccacttctg tcagtgatag
     1681 taatttttcc tctgaagagc cttcaaggaa aaatcccaca cgctggtcaa caacagctgg
     1741 cttcctcaag cagaacactg ctgggagtcc taaaacctgc gacaggaaga aagatgaaaa
     1801 ctacagaagc ctcccgcggg atactagtaa ctggtctaac caatttcaga gagacaatgc
     1861 tcgctcgtct ctgagtgcca gtcacccaat ggtgggcaag tggctggaga aacaagaaca
     1921 ggatgaggat gggacagaag aggataacag tcgtgttgaa cctgttggac atgctgacac
     1981 gggtttggag catataccca acttttctct ggatgatatg gtaaagctcg tagaagtccc
     2041 caacgatgga gggcctctgg gaatccatgt agtgcctttc agtgctcgag gcggcagaac
     2101 cctggggtta ttagtaaaac gattggagaa aggtggtaaa gctgaacatg aaaatctttt
     2161 tcgtgagaat gattgcattg tcaggattaa tgatggcgac cttcgaaata gaagatttga
     2221 acaagcacaa catatgtttc gccaagccat gcgtacaccc atcatttggt tccatgtggt
     2281 tcctgcagca aataaagagc agtatgaaca actatcccaa agtgagaaga acaattacta
     2341 ttcaagccgt tttagccctg acagccagta tattgacaac aggagtgtga acagtgcagg
     2401 gcttcacacg gtgcagagag caccccgact gaaccacccg cctgagcaga tagactctca
     2461 ctcaagacta cctcatagcg cacacccctc gggaaaacca ccatccgctc cagcctcggc
     2521 acctcagaat gtatttagta cgactgtaag cagtggttat aacaccaaaa aaataggcaa
     2581 gaggcttaat atccagctta agaaaggtac agaaggtttg ggattcagca tcacttccag
     2641 agatgtaaca ataggtggct cagctccaat ctatgtgaaa aacattctcc cccggggggc
     2701 ggccattcag gatggccgac ttaaggcagg agacagactt atagaggtaa atggagtaga
     2761 tttagtgggc aaatcccaag aggaagttgt ttcgctgttg agaagcacca agatggaagg
     2821 aactgtgagc cttctggtct ttcgccagga agacgccttc cacccaaggg aactgaatgc
     2881 agagccaagc cagatgcaga ttccaaaaga aacgaaagca gaagatgagg atattgttct
     2941 tacacctgat ggcaccaggg aatttctgac atttgaagtc ccacttaatg attcaggatc
     3001 tgcaggcctt ggtgtcagtg tcaaaggtaa ccggtcaaaa gagaaccacg cagatttggg
     3061 aatctttgtc aagtccatta ttaatggagg agcagcatct aaagatggaa ggcttcgggt
     3121 gaatgatcaa ctgatagcag taaatggaga atccctgttg ggcaagacaa accaagatgc
     3181 catggaaacc ctaagaaggt ctatgtctac tgaaggcaat aaacgaggaa tgatccagct
     3241 tattgttgca aggagaataa gcaagtgcaa tgagctgaag tcacctggga gcccccctgg
     3301 acctgagctg cccattgaaa cagcgttgga tgatagagaa cgaagaattt cccattccct
     3361 ctacagtggg attgaggggc ttgatgaatc gcccagcaga aatgctgccc tcagtaggat
     3421 aatgggtaaa taccagctgt cccctacagt gaatatgccc caagatgaca ctgtcattat
     3481 agaagatgac aggttgccag tgcttcctcc acatctctct gaccagtcct cttccagctc
     3541 ccatgatgat gtggggtttg tgacggcaga tgctggtact tgggccaagg ctgcaatcag
     3601 tgattcagcc gactgctctt tgagtccaga tgttgatcca gttcttgctt ttcaacgaga
     3661 aggatttgga cgtcagagta tgtcagaaaa acgcacaaag caattttcag atgccagtca
     3721 attggatttc gttaaaacac gaaaatcaaa aagcatggat ttaggtatag ctgacgagac
     3781 taaactcaat acagtggatg accagaaagc aggttctccc agcagagatg tgggtccttc
     3841 cctgggtctg aagaagtcaa gctcgttgga gagtctgcag accgcagttg ccgaggtgac
     3901 tttgaatggg gatattcctt tccatcgtcc acggccgcgg ataatcagag gcaggggatg
     3961 caatgagagc ttcagagctg ccatcgacaa atcttatgat aaacccgcgg tagatgatga
     4021 tgatgaaggc atggagacct tggaagaaga cacagaagaa agttcaagat cagggagaga
     4081 gtctgtatcc acagccagtg atcagccttc ccactctctg gagagacaaa tgaatggaaa
     4141 ccaagagaaa ggtgataaga ctgatagaaa aaaggataaa actggaaaag aaaagaagaa
     4201 agatagagat aaggagaagg ataaaatgaa agccaagaag ggaatgctga agggcttggg
     4261 agacatgttc aggtttggca aacatcgaaa agatgacaag attgagaaaa cgggtaaaat
     4321 aaaaatacag gaatccttta catcagaaga ggagaggata cgaatgaagc aggagcagga
     4381 gaggattcaa gccaaaactc gagaatttag ggaacgacaa gctcgagagc gtgactatgc
     4441 tgaaattcaa gattttcatc ggacatttgg ctgtgatgat gagttaatgt atgggggagt
     4501 ttcttcttat gaaggttcca tggctctcaa cgctagacct cagagcccac gagaagggca
     4561 tatgatggat gctttgtatg cccaagtcaa gaagccgcgg aattccaaac cctcacctgt
     4621 agacagtaac agatcaactc ctagcaatca tgatcggata cagcgtctga ggcaagaatt
     4681 tcagcaagca aagcaagatg aagatgtaga agatcgtcgg cggacctata gttttgagca
     4741 accctggccg aacgcacggc cggcgacgca gagcgggcga cactcggtgt ccgtggaggt
     4801 gcagatgcag cggcagcggc aggaggagcg cgagagctcc cagcaggccc agcgccagta
     4861 cagctctctg cctcggcaaa gcaggaaaaa tgccagctcg gtctcccagg actcttggga
     4921 gcagaactac tcccctgggg aaggcttcca gagtgccaaa gagaacccca ggtactccag
     4981 ctaccaaggc tccaggaacg gctacctggg aggacatggc ttcaacgcca gggtcatgct
     5041 ggaaactcag gagctccttc gccaggaaca gaggcgaaag gagcagcaga tgaagaagca
     5101 gcctccttcc gaggggccca gcaactatga ctcgtataag aaagtccagg accccagtta
     5161 cgcccctccc aaggggccct tccggcaaga tgtgcccccc tccccttctc aggttgcgag
     5221 gctgaacaga cttcagactc ctgagaaagg gaggcccttc tattcctgag cggccgcttg
     5281 aattcatcga tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata
     5341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5401 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc
     5461 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     5521 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc
     5881 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5941 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6001 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     6061 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     6121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6301 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     6361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6421 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     6481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6541 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     6601 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6661 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6721 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6781 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6901 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6961 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7021 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7081 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7141 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7201 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7261 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7321 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7381 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7441 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7501 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7561 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7621 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7681 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7741 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7801 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8101 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8761 gcccaatacg caaaccgcct c
//
