LOCUS       sequence-195138-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLenti-Crispr-V2-HMID.v1
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    212..329
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        380..628
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     380..400
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     551..570
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        650..725
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcnccat tatcgtttca
       61 gacccanctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
      121 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
      181 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      241 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
      301 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
      361 tttggttaat taaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg
      421 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
      481 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      541 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      601 atatatcttg tggaaaggac gaaacaccgg gcactgcggc tggaggtggg ttttagagct
      661 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      721 ggtgcttttt tgaattcgct agctaggtct tgaaaggagt gggaattggc tccggtgccc
      781 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      841 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      901 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      961 acgttctttt tcgcaacggg tttgccgcca gaacac
//
