LOCUS       sequence-195139-       11252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Wong AS, Choi GC, Cheng AA, Purcell O, Lu TK
  TITLE     Massively parallel high-order combinatorial genetics in human cells.
  JOURNAL   Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 
            2015 Aug 17.
  PUBMED    26280411
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3326"; 
            journalName: "Nat Biotechnol"; date: "2015-09"; volume: "33"; issue:
            "9"; pages: "952-61"
COMMENT     Sequence Label: pAWp7
FEATURES             Location/Qualifiers
     source          1..11252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        117..496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             714..894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    941..1066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1559..1792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1977..2021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2319..2436
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2501..3712
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3549..3568
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3729..4424
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     enhancer        4455..4834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4835..5038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5057..5066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5063..5065,5066..5068,5069..5782)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5063..5065,5066..5068,5069..5779)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5108..5129)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5369..5388)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5716..5737
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5823..6411
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5876..5896)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6294..6305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6414..6430)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6415..6431)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6936..7116
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7142..7159)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7148..7372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7418..7846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7505..7524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7715..7736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7855..7875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7860..8189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8040..8175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8102..8121
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8237..8284
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8303..8677
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8807..8928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8844..8863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8898..8917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8977..8993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8977..8993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8990..9012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9001..9017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9025..9031,9032..9049,9050..9055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9070..9091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9208..9225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9379..9967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9459..9478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10138..10929,10930..10998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10761..10780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10999..11103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11178..11197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
       61 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      121 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      541 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      601 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      661 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      901 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      961 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1021 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1081 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1141 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1201 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1261 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1321 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1381 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1441 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1501 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1561 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1621 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1681 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1741 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1801 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1861 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1921 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1981 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2041 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2101 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2161 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2221 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2281 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2341 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2401 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2461 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg
     2521 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     2581 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     2641 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     2701 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     2761 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     2821 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     2881 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     2941 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     3001 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     3061 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     3121 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     3181 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     3241 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     3301 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     3361 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     3421 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     3481 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     3541 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     3601 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     3661 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acagttaatt
     3721 aagccaccat gagcgagctg atcaaggaga acatgcacat gaagctgtac atggagggca
     3781 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     3841 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     3901 tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg
     3961 acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag
     4021 acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca
     4081 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     4141 gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca
     4201 gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat
     4261 acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca
     4321 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
     4381 tggccaagta ctgcgacctc cctagcaaac tggggcacag ataataacct gcaggcgggc
     4441 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     4501 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     4561 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4621 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4681 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4741 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     4801 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     4861 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     4921 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     4981 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcggtaa
     5041 cccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5101 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5161 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5221 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5281 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5341 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5401 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5461 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5521 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5581 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5641 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5701 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5761 catggacgag ctgtacaagt aaagcggccg cgactctaga attcgatatc aagcttatcg
     5821 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5881 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5941 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6001 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6061 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6121 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6181 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6241 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6301 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6361 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     6421 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6481 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6541 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6601 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6661 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6721 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6781 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6841 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6901 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7081 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7141 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7201 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7261 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7321 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7381 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7441 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7501 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7561 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7621 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7681 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7741 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7801 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7861 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7921 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8041 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8101 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8161 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8221 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8281 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8341 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8401 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8461 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8521 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8581 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8641 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8701 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8761 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8821 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8881 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8941 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11101 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    11161 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    11221 cctgcttgtg tgttggaggt cgctgagtag tg
//
