LOCUS       sequence-195141-       11264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, 
            Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK
  TITLE     Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
  PUBMED    26864203
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 10. pii: 201517883."
COMMENT     Sequence Label: pAWp9
FEATURES             Location/Qualifiers
     source          1..11264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        469..667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             685..865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    912..1037
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1530..1763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1948..1992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2290..2407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2472..3683
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3520..3539
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3700..4395
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     enhancer        4426..4805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4806..5009
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5022..5031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5028..5030,5031..5033,5034..5747)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5028..5030,5031..5033,5034..5744)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5073..5094)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5334..5353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5681..5702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5806..6394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5859..5879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6277..6288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6397..6413)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6398..6414)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6919..7099
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7125..7142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7131..7355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7401..7829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7488..7507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7698..7719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7838..7858)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7843..8172
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8023..8158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8085..8104
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8220..8267
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8286..8660
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8790..8911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8827..8846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8881..8900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8960..8976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8960..8976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8973..8995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8984..9000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9008..9014,9015..9032,9033..9038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9053..9074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9191..9208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9362..9950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9442..9461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10121..10912,10913..10981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10744..10763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10982..11086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11161..11180)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
       61 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      481 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      541 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      601 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      661 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      721 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      781 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      841 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      901 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      961 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1021 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1081 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1141 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1201 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1261 atacagtagc aaccatctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1321 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1381 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1441 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1501 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1561 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1621 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1681 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1741 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1801 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1861 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     1921 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     1981 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2041 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2101 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2161 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2221 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     2281 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2341 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2401 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta acccgtgtcg
     2461 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2521 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2581 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2641 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2701 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2761 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2821 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2881 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2941 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     3001 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     3061 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     3121 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3181 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3241 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3301 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3361 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3421 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3481 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3541 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3601 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3661 cgtttttggc ttttttgtta gacagttaat taagccacca tgagcgagct gatcaaggag
     3721 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     3781 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgaagatcaa ggtggtcgag
     3841 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     3901 gccttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc
     3961 ttcacatggg agagaatcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     4021 accagcttcc agaacggctg catcatctac aacgtcaaga tcaacggggt gaacttccca
     4081 tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg aggccaacac cgagatgctg
     4141 taccccgctg acggcggcct gagaggccac agccagatgg ccctgaagct cgtgggcggg
     4201 ggctacctgc actgctcctt caagaccaca tacagatcca agaaacccgc taagaacctc
     4261 aagatgcccg gcttccactt cgtggaccac agactggaaa gaatcaagga ggccgacaaa
     4321 gagacctacg tcgagcagca cgagatggct gtggccaagt actgcgacct ccctagcaaa
     4381 ctggggcaca gataataacc tgcaggcggg ccagatatac gcgttgacat tgattattga
     4441 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4501 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4561 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4621 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4681 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4741 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4801 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4861 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4921 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4981 tacggtggga ggtctatata agcagcggta acctaccggt cgccaccatg gtgagcaagg
     5041 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5101 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5161 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5221 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5281 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5341 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5401 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5461 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     5521 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5581 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5641 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5701 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     5761 ctaggatccg cgaaaagtcc ggaattcgat atcaagctta tcgataatca acctctggat
     5821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6181 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6361 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     6421 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6481 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6541 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6601 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     6661 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     6721 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     6781 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     6841 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     6901 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7081 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     7141 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7201 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7261 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7321 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7381 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7441 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7501 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7561 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7621 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7681 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7741 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7801 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7921 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7981 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8101 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8161 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     8221 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     8281 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     8341 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     8401 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     8461 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8521 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8581 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     8641 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     8701 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8761 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8941 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9061 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9121 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9181 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9241 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9781 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11101 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    11161 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    11221 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggc
//
