LOCUS       sequence-195154-       10451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of IKZF3 with Q147H mutation and FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..97
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1738..1762)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1782..1805
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1840..2389
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1984..2001)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2211..2230
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2392..2394,2395..2397,2398..3111)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2392..2394,2395..2397,2398..3108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2437..2458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2698..2717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3045..3066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3188..3776
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3241..3261)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3659..3670)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3779..3795)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3780..3796)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3985..4218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4290..4411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4327..4346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4381..4400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4451..4586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4606..4625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4607..4625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4648..5006)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4688..4709)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4900..4919
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5033..5137
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5139..5496
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5139..5330
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5162..5182)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5347..5482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5531..6325
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5531..6325
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5585..5604)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6195..6214
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6513..6532)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6557..6604
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6933..7521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7422..7441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7696..7999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8001..8199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8154..8174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8217..8397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8444..8569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9066..9299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9484..9528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9826..9943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10041..10420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
       61 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
      121 attcccctag ccgcggaatc tagactgccg gatcgaattg tcgacgaatt cggatctggc
      181 caatcacaag tttgtacaaa aaagttggca tggaagatat acaaacaaat gcggaactga
      241 aaagcactca ggagcagtct gtgcccgcag aaagtgcagc ggttttgaat gactacagtt
      301 taaccaaatc tcatgaaatg gaaaatgtgg acagtggaga aggcccagcc aatgaagatg
      361 aagacatagg agatgattca atgaaagtga aagatgaata cagtgaaaga gatgagaatg
      421 ttttaaagtc agaacccatg ggaaatgcag aagagcctga aatcccttac agctattcaa
      481 gagaatataa tgaatatgaa aacattaagt tggagagaca tgttgtctca ttcgatagta
      541 gcaggccaac cagtggaaag atgaactgcg atgtgtgtgg attatcctgc atcagcttca
      601 atgtcttaat ggttcataag cgaagccata ctggtgaacg cccattccac tgtaatcagt
      661 gtggggcatc ttttactcag aaaggtaacc tcctccgcca cattaaactg cacacagggg
      721 aaaaaccttt taagtgtcac ctctgcaact atgcatgcca aagaagagat gcgctcacgg
      781 ggcatcttag gacacattct gtggagaaac cctacaaatg tgagttttgt ggaaggagtt
      841 acaagcagag aagttccctt gaggagcaca aggagcgctg ccgtacattt cttcagagca
      901 ctgacccagg ggacactgca agtgcggagg caagacacat caaagcagag atgggaagtg
      961 aaagagctct cgtactggac agattagcaa gcaatgtggc aaaacgaaaa agctcaatgc
     1021 ctcagaaatt cattggtgag aagcgccact gctttgatgt caactataat tcaagttaca
     1081 tgtatgagaa agagagtgag ctcatacaga cccgcatgat ggaccaagcc atcaataacg
     1141 ccatcagcta tcttggcgcc gaagccctgc gccccttggt ccagacaccg cctgctccca
     1201 cctcggagat ggttccagtt atcagcagca tgtatcccat agccctcacc cgggctgaga
     1261 tgtcaaacgg tgcccctcaa gagctggaaa agaaaagcat ccaccttcca gagaagagcg
     1321 tgccttctga gagaggcctc tctcccaaca atagtggcca cgactccacg gacactgaca
     1381 gcaaccatga agaacgccag aatcacatct atcagcaaaa tcacatggtc ctgtctcggg
     1441 cccgcaatgg gatgccactt ctgaaggagg ttccccgctc ttacgaactc ctcaagcccc
     1501 cgcccatctg cccaagagac tccgtcaaag tgatcaacaa ggaaggggag gtgatggatg
     1561 tgtatcggtg tgaccactgc cgcgtcctct tcctggacta tgtgatgttc acgattcaca
     1621 tgggctgcca cggcttccgt gaccctttcg agtgtaacat gtgtggatat cgaagccatg
     1681 atcggtatga gttctcgtct cacatagcca gaggagaaca cagagccctg ctgaagtgcc
     1741 caactttctt gtacaaagtg gtgggaggag gtggatctgg cgactacaag gacgacgatg
     1801 acaagtgact cgagcgggat caattccgcc ccccccctaa cgttactggc cgaagccgct
     1861 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     1921 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     1981 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2041 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     2101 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     2161 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     2221 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     2281 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     2341 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catggtgagc
     2401 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2461 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2521 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2581 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2641 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2701 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2761 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2821 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2881 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2941 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3001 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3061 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     3121 gactctagag tcgacctgca ggcatgcaag cttcaggtag ccggctaacg ttatcaagct
     3181 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3241 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3301 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3361 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3421 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3481 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3541 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3601 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3661 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3721 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3781 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     3841 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     3901 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4561 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4621 tattagcatg tgagcaaaag gccagcataa atcaaaagaa tagaccgaga tagggttgag
     4681 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4741 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4801 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4861 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4921 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4981 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     5041 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5101 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     5161 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5221 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5341 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5401 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5461 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5521 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5581 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5641 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5701 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5761 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5821 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5881 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5941 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6001 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     6061 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6121 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6181 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6241 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6301 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6361 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6421 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6481 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6541 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6601 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6661 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6721 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     6781 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6841 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6901 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6961 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7021 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7081 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7141 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7201 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7261 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7321 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7381 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7441 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7501 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7561 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7621 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     7681 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     7741 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     7801 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     7861 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7921 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     7981 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     8041 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     8101 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     8161 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     8221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8401 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8461 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8521 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8581 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8641 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8701 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8761 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8821 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8881 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     8941 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9001 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9061 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9121 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9181 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9241 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9301 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9361 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9421 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9481 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9541 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9601 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9661 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     9721 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     9781 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     9841 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     9901 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     9961 cagcagagat ccagtttggt tagtaccggg cccgctctag ccctagattt aaatcgatgt
    10021 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
    10081 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    10141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    10201 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    10261 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    10321 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    10381 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
    10441 ctgcttcact c
//
