LOCUS       sequence-195157-       11609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of IKZF2 variant 2 with FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF2-V2-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1000
                     /label=chicken beta-actin promoter
     intron          1001..2009
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1933..1955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2017..2036
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2125..2149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3650..3674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3694..3717
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3752..4301
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3896..3913)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4123..4142
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4304..4306,4307..4309,4310..5023)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4304..4306,4307..4309,4310..5020)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4349..4370)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4610..4629)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4957..4978
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5100..5688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5153..5173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5571..5582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5691..5707)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5692..5708)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5897..6130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6202..6323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6239..6258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6293..6312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6363..6498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6518..6537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6519..6537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6560..6918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6600..6621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6812..6831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6945..7049
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7051..7408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7051..7242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7074..7094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7259..7394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7443..8237
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7443..8237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7497..7516)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8107..8126
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8425..8444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8469..8516
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8845..9433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9334..9353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9608..9911
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9913..10111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10066..10086
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10129..10309
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10356..10481
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10978..11211
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11396..11440
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc
     1021 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     1081 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     1141 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     1201 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     1261 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1321 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1381 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1441 tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac
     1501 ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc
     1561 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1621 tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1681 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1741 ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg
     1801 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1861 ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac
     1921 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1981 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2041 ctcatcattt tggcaaagaa ttcccctagc cgcggaatct agactgccgg atcgaattgt
     2101 cgacgaattc ggatctggcc aatcacaagt ttgtacaaaa aagttggcat ggaaacagag
     2161 gccattgatg gctatataac gtgtgacaat gagctttcac ccgaaaggga gcactccaat
     2221 atggcaattg acctcacctc aagcacaccc aatggacagc atgcctcacc aagtcacatg
     2281 acaagcacaa attcagtaaa gctagaaatg cagagtgatg aagagtgtga caggaaaccc
     2341 ctgagccgtg aagatgagat caggggccat gatgagggta gcagcctaga agaaccccta
     2401 attgagagca gcgaggtggc tgacaacagg aaagtccagg agcttcaagg cgagggagga
     2461 atccggcttc cgaatggtga acgccccttc cactgtaacc agtgtggagc ttcttttact
     2521 cagaagggca accttctgag acacataaag ttacactctg gagagaagcc gttcaaatgt
     2581 cctttctgta gctacgcctg tagaagaagg gacgccctca caggacacct caggacccat
     2641 tctgtgggta aacctcacaa gtgcaactac tgtggacgaa gctacaagca gcgcagttca
     2701 ctggaggagc acaaggaacg ctgccacaac tatctccaga atgtcagcat ggaggctgct
     2761 gggcaggtca tgagtcacca tgtacctcct atggaagatt gtaaggaaca agagcctatt
     2821 atggacaaca atatttctct ggtgcctttt gagagacctg ctgtcataga gaagctcacg
     2881 gggaatatgg gaaaacgtaa aagctccact ccacaaaagt ttgtggggga aaagctcatg
     2941 cgattcagct acccagatat tcactttgat atgaacttaa catatgagaa ggaggctgag
     3001 ctgatgcagt ctcatatgat ggaccaagcc atcaacaatg caatcaccta ccttggagct
     3061 gaggcccttc accctctgat gcagcacccg ccaagcacaa tcgctgaagt ggccccagtt
     3121 ataagctcag cttattctca ggtctatcat ccaaatagga tagaaagacc cattagcagg
     3181 gaaactgctg atagtcatga aaacaacatg gatggcccca tctctctcat cagaccaaag
     3241 agtcgacccc aggaaagaga ggcctctccc agcaatagct gcctggattc cactgactca
     3301 gaaagcagcc atgatgacca ccagtcctac caaggacacc ctgccttaaa tcccaagagg
     3361 aaacaaagcc cagcttacat gaaggaggat gtcaaagctt tggatactac caaggctcct
     3421 aagggctctc tgaaggacat ctacaaggtc ttcaatggag aaggagaaca gattagggcc
     3481 ttcaagtgtg agcactgccg agtccttttc ctagaccatg tcatgtacac cattcacatg
     3541 ggttgccatg gctaccggga cccactggaa tgcaacatct gtggctacag aagccaggac
     3601 cgttatgagt tttcatcaca cattgttcga ggggagcaca cattccacta cccaactttc
     3661 ttgtacaaag tggtgggagg aggtggatct ggcgactaca aggacgacga tgacaagtga
     3721 ctcgagcggg atcaattccg ccccccccct aacgttactg gccgaagccg cttggaataa
     3781 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3841 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3901 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3961 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     4021 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     4081 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     4141 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     4201 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     4261 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggtga gcaagggcga
     4321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4441 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4501 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4741 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4801 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4861 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4921 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4981 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     5041 agtcgacctg caggcatgca agcttcaggt agccggctaa cgttatcaag cttatcgata
     5101 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5161 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5221 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5281 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5341 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5401 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5461 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5521 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5581 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5641 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5701 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     5761 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     5821 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5881 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5941 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6001 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6061 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6121 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6181 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6301 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6361 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6421 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6481 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattagca
     6541 tgtgagcaaa aggccagcat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6901 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7021 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     7081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7381 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     7441 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7501 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7561 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7621 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7681 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7741 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7801 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7861 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7921 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     7981 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8041 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8101 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8161 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8221 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     8281 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8401 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     8461 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     8521 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     8581 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     8641 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     8701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9541 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9601 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9661 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     9721 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     9781 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     9841 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     9901 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     9961 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    10021 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    10081 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
    10141 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10201 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10261 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10321 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    10381 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    10441 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
    10501 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
    10561 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    10621 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    10681 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    10741 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    10801 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
    10861 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10921 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10981 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    11041 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11101 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    11161 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    11221 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    11281 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    11341 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    11401 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    11461 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    11521 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    11581 gtttcagacc cacctcccaa ccccgaggg
//
