LOCUS       sequence-195158-       10463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of IKZF2 variant 2 with FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF2-V2-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..56
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     118..137
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    226..250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1751..1775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1795..1818
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1853..2402
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1997..2014)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2224..2243
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2405..2407,2408..2410,2411..3124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2405..2407,2408..2410,2411..3121)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2450..2471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2711..2730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3058..3079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3201..3789
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3254..3274)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3672..3683)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3792..3808)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3793..3809)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3998..4231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4303..4424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4340..4359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4394..4413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4464..4599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4619..4638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4620..4638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4661..5019)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4701..4722)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4913..4932
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5046..5150
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5152..5509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5152..5343
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5175..5195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5360..5495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5544..6338
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5544..6338
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5598..5617)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6208..6227
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6526..6545)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6570..6617
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6946..7534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7435..7454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7709..8012
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8014..8212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8167..8187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8230..8410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8457..8582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9079..9312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9497..9541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9839..9956
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10054..10433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
       61 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
      121 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcccctag ccgcggaatc
      181 tagactgccg gatcgaattg tcgacgaatt cggatctggc caatcacaag tttgtacaaa
      241 aaagttggca tggaaacaga ggccattgat ggctatataa cgtgtgacaa tgagctttca
      301 cccgaaaggg agcactccaa tatggcaatt gacctcacct caagcacacc caatggacag
      361 catgcctcac caagtcacat gacaagcaca aattcagtaa agctagaaat gcagagtgat
      421 gaagagtgtg acaggaaacc cctgagccgt gaagatgaga tcaggggcca tgatgagggt
      481 agcagcctag aagaacccct aattgagagc agcgaggtgg ctgacaacag gaaagtccag
      541 gagcttcaag gcgagggagg aatccggctt ccgaatggtg aacgcccctt ccactgtaac
      601 cagtgtggag cttcttttac tcagaagggc aaccttctga gacacataaa gttacactct
      661 ggagagaagc cgttcaaatg tcctttctgt agctacgcct gtagaagaag ggacgccctc
      721 acaggacacc tcaggaccca ttctgtgggt aaacctcaca agtgcaacta ctgtggacga
      781 agctacaagc agcgcagttc actggaggag cacaaggaac gctgccacaa ctatctccag
      841 aatgtcagca tggaggctgc tgggcaggtc atgagtcacc atgtacctcc tatggaagat
      901 tgtaaggaac aagagcctat tatggacaac aatatttctc tggtgccttt tgagagacct
      961 gctgtcatag agaagctcac ggggaatatg ggaaaacgta aaagctccac tccacaaaag
     1021 tttgtggggg aaaagctcat gcgattcagc tacccagata ttcactttga tatgaactta
     1081 acatatgaga aggaggctga gctgatgcag tctcatatga tggaccaagc catcaacaat
     1141 gcaatcacct accttggagc tgaggccctt caccctctga tgcagcaccc gccaagcaca
     1201 atcgctgaag tggccccagt tataagctca gcttattctc aggtctatca tccaaatagg
     1261 atagaaagac ccattagcag ggaaactgct gatagtcatg aaaacaacat ggatggcccc
     1321 atctctctca tcagaccaaa gagtcgaccc caggaaagag aggcctctcc cagcaatagc
     1381 tgcctggatt ccactgactc agaaagcagc catgatgacc accagtccta ccaaggacac
     1441 cctgccttaa atcccaagag gaaacaaagc ccagcttaca tgaaggagga tgtcaaagct
     1501 ttggatacta ccaaggctcc taagggctct ctgaaggaca tctacaaggt cttcaatgga
     1561 gaaggagaac agattagggc cttcaagtgt gagcactgcc gagtcctttt cctagaccat
     1621 gtcatgtaca ccattcacat gggttgccat ggctaccggg acccactgga atgcaacatc
     1681 tgtggctaca gaagccagga ccgttatgag ttttcatcac acattgttcg aggggagcac
     1741 acattccact acccaacttt cttgtacaaa gtggtgggag gaggtggatc tggcgactac
     1801 aaggacgacg atgacaagtg actcgagcgg gatcaattcc gccccccccc taacgttact
     1861 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     1921 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     1981 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     2041 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     2101 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     2161 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     2221 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     2281 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     2341 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     2401 taccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2521 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3121 gtaaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttcagg tagccggcta
     3181 acgttatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3241 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3301 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3361 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3421 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3481 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3541 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3601 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3661 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3721 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3781 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     3841 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     3901 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     3961 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4021 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4201 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4261 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     4321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     4441 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4501 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4561 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     4621 ctatagtgag tcgtattagc atgtgagcaa aaggccagca taaatcaaaa gaatagaccg
     4681 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4741 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4801 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4861 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4921 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4981 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     5041 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5101 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     5161 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     5521 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5581 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5641 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5701 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     5761 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5821 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5881 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5941 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     6001 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     6061 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6121 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6181 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6241 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6301 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6361 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6421 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6481 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     6541 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     6601 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     6661 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     6721 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     6781 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7561 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7621 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     7681 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7741 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7801 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7861 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7921 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     8101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     8221 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8281 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8341 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8401 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8461 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8521 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8581 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8641 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8701 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8761 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8821 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8881 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     8941 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9001 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9061 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9121 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9181 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9241 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9301 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9361 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9421 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9481 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9541 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9601 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9661 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9721 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9781 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     9841 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9901 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9961 tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc tagccctaga
    10021 tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
    10081 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
    10141 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
    10201 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
    10261 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
    10321 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
    10381 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
    10441 tgagccccac gttctgcttc act
//
