LOCUS       sequence-195160-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes FKBP-dCas9(N) fused to VEGFR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..574
                     /label=chicken beta-actin promoter
     intron          575..802
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             3640..3960
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3967..3993
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4009..4032,4033..4035,4036..5610)
                     /codon_start=1
                     /product="N-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the D10A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     CDS             5641..5697
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5704..5723)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6200..6220
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6337..6357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6755..6766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6897..6914)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6903..7110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7119..7259
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7119..7248
                     /label=AAV2 ITR
     rep_origin      7334..7789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7421..7440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7631..7652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7806..7825)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7925..7947
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7985..8003)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8071..8175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8176..8244,8245..9036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8394..8413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9207..9795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9696..9715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      421 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      481 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      541 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      601 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      661 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc tgagcaagag
      721 gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc tggagcacct
      781 gcctgaaatc actttttttc aggttggacc ggtgccacca tgcagagcaa ggtgctgctg
      841 gccgtcgccc tgtggctctg cgtggagacc cgggccgcct ctgtgggttt gcctagtgtt
      901 tctcttgatc tgcccaggct cagcatacaa aaagacatac ttacaattaa ggctaataca
      961 actcttcaaa ttacttgcag gggacagagg gacttggact ggctttggcc caataatcag
     1021 agtggcagtg agcaaagggt ggaggtgact gagtgcagcg atggcctctt ctgtaagaca
     1081 ctcacaattc caaaagtgat cggaaatgac actggagcct acaagtgctt ctaccgggaa
     1141 actgacttgg cctcggtcat ttatgtctat gttcaagatt acagatctcc atttattgct
     1201 tctgttagtg accaacatgg agtcgtgtac attactgaga acaaaaacaa aactgtggtg
     1261 attccatgtc tcgggtccat ttcaaatctc aacgtgtcac tttgtgcaag atacccagaa
     1321 aagagatttg ttcctgatgg taacagaatt tcctgggaca gcaagaaggg ctttactatt
     1381 cccagctaca tgatcagcta tgctggcatg gtcttctgtg aagcaaaaat taatgatgaa
     1441 agttaccagt ctattatgta catagttgtc gttgtagggt ataggattta tgatgtggtt
     1501 ctgagtccgt ctcatggaat tgaactatct gttggagaaa agcttgtctt aaattgtaca
     1561 gcaagaactg aactaaatgt ggggattgac ttcaactggg aatacccttc ttcgaagcat
     1621 cagcataaga aacttgtaaa ccgagaccta aaaacccagt ctgggagtga gatgaagaaa
     1681 tttttgagca ccttaactat agatggtata acccggagtg accaaggatt gtacacctgt
     1741 gcagcatcca gtgggctgat gaccaagaag aacagcacat ttgtcagggt ccatgaaaaa
     1801 ccttttgttg cttttggaag tggcatggaa tctctggtgg aagccacggt gggggagcgt
     1861 gtcagaatcc ctgcgaagta ccttggttac ccacccccag aaataaaatg gtataaaaat
     1921 ggaatacccc ttgagtccaa tcacacaatt aaagcggggc atgtactgac gattatggaa
     1981 gtgagtgaaa gagacacagg aaattacact gtcatcctta ccaatcccat ttcaaaggag
     2041 aagcagagcc atgtggtctc tctggttgtg tatgtcccac cccagattgg tgagaaatct
     2101 ctaatctctc ctgtggattc ctaccagtac ggcaccactc aaacgctgac atgtacggtc
     2161 tatgccattc ctcccccgca tcacatccac tggtattggc agttggagga agagtgcgcc
     2221 aacgagccca gccaagctgt ctcagtgaca aacccatacc cttgtgaaga atggagaagt
     2281 gtggaggact tccagggagg aaataaaatt gaagttaata aaaatcaatt tgctctaatt
     2341 gaaggaaaaa acaaaactgt aagtaccctt gttatccaag cggcaaatgt gtcagctttg
     2401 tacaaatgtg aagcggtcaa caaagtcggg agaggagaga gggtgatctc cttccacgtg
     2461 accaggggtc ctgaaattac tttgcaacct gacatgcagc ccactgagca ggagagcgtg
     2521 tctttgtggt gcactgcaga cagatctacg tttgagaacc tcacatggta caagcttggc
     2581 ccacagcctc tgccaatcca tgtgggagag ttgcccacac ctgtttgcaa gaacttggat
     2641 actctttgga aattgaatgc caccatgttc tctaatagca caaatgacat tttgatcatg
     2701 gagcttaaga atgcatcctt gcaggaccaa ggagactatg tctgccttgc tcaagacagg
     2761 aagaccaaga aaagacattg cgtggtcagg cagctcacag tcctagagcg tgtggcaccc
     2821 acgatcacag gaaacctgga gaatcagacg acaagtattg gggaaagcat cgaagtctca
     2881 tgcacggcat ctgggaatcc ccctccacag atcatgtggt ttaaagataa tgagaccctt
     2941 gtagaagact caggcattgt attgaaggat gggaaccgga acctcactat ccgcagagtg
     3001 aggaaggagg acgaaggcct ctacacctgc caggcatgca gtgttcttgg ctgtgcaaaa
     3061 gtggaggcat ttttcataat agaaggtgcc caggaaaaga cgaacttgga aatcattatt
     3121 ctagtaggca cggcggtgat tgccatgttc ttctggctac ttcttgtcat catcggcggt
     3181 ggctcgggtg gtggctcggg agaaagcttg tttaaggggc cgcgtgatta caacccgata
     3241 tcgagcacca tttgtcattt gacgaatgaa tctgatgggc acacaacatc gttgtatggt
     3301 attggatttg gtcccttcat cattacaaac aagcacttgt ttagaagaaa taatggaaca
     3361 ctgttggtcc aatcactaca tggtgtattc aaggtcaaga acaccacgac tttgcaacaa
     3421 cacctcattg atgggaggga catgataatt attcgcatgc ctaaggattt cccaccattt
     3481 cctcaaaagc tgaaatttag agagccacaa agggaagagc gcatatgtct tgtgacaacc
     3541 aacttccaaa ctggcggtgg ctcgctagat ttagctagct taatcctcgg caagcttggc
     3601 gaaaacctgt attttcagct aggcggaggc agcactagtg gcgtgcaggt ggagaccatc
     3661 agccccggcg acggcagaac cttccccaag agaggccaga cctgcgtggt gcactacacc
     3721 ggcatgctgg aggacggcaa gaagttcgac agcagcagag acagaaacaa gcccttcaag
     3781 ttcatgctgg gcaagcagga ggtgatcaga ggctgggagg agggcgtggc ccagatgagc
     3841 gtgggccaga gagccaagct gaccatcagc cccgactacg cctacggcgc caccggccac
     3901 cccggcatca tccctcctca cgccaccctg gtgttcgacg tggagctgct gaagctggag
     3961 actagttacc catacgacgt accagattac gctggaggtt ctggatccga caagaagtac
     4021 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     4081 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     4141 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     4201 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     4261 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     4321 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     4381 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     4441 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     4501 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     4561 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     4621 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     4681 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc
     4741 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     4801 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     4861 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     4921 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     4981 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     5041 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     5101 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     5161 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     5221 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     5281 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     5341 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     5401 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     5461 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     5521 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     5581 aaagtgaaat acgtgaccga gggaatgaga ggtggcggtg gctcgggtac cggatctgga
     5641 gccaccaatt tttctctgct gaagcaggct ggtgatgttg aagaaaaccc tggacctgaa
     5701 ttcatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5761 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5821 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5881 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5941 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6001 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6061 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6121 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6181 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6241 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa ggggtaacta
     6301 agtaaggatc gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg
     6361 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6421 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6481 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6541 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6661 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6721 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6781 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6841 gagtcggatc tccctttggg ccgcctcccc gccaattcct agagctcgct gatcagcctc
     6901 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6961 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7021 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7081 ttgggaagag aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt
     7141 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     7201 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     7261 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     7321 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7381 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     7441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     7561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     7681 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     7741 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     7801 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7861 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7921 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7981 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8041 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8101 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8161 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8221 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8281 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8341 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8401 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8461 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8521 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8581 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8641 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8701 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8761 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8821 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8881 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8941 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9001 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9061 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9121 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9841 ccttttgctc acat
//
