LOCUS       sequence-195161-       13662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV-1 delta Rev was cloned into pSC101 and modified to include an 
            RTEm26CTE element in order to utilize the NXF1 RNA export pathway. 
            The RRE was also mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 13662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pSC101 NL4-3 envFS Delta-rev Delta-RRE RTEm26CTE
FEATURES             Location/Qualifiers
     source          1..13662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..80
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     LTR             477..734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             890..2392
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2185..5196
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4881..4998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             8279..8323
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8617..8643
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             9617..10250
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     terminator      complement(10317..10344)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(10883..11833)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      11881..12103
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(12595..12689)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(12720..13514)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12831..12850)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13441..13460
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc gcgcgcacgc gtttaattaa tggaagggct aatttggtcc
      121 caaaaaagac aagagatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
      181 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgcttc
      241 aagttagtac cagttgaacc agagcaagta gaagaggcca atgaaggaga gaacaacagc
      301 ttgttacacc ctatgagcca gcatgggatg gaggacccgg agggagaagt attagtgtgg
      361 aagtttgaca gcctcctagc atttcgtcac atggcccgag agctgcatcc ggagtactac
      421 aaagactgct gacatcgagc tttctacaag ggactttccg ctggggactt tccagggagg
      481 tgtggcctgg gcgggactgg ggagtggcga gccctcagat gctacatata agcagctgct
      541 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgctca aagtagtgtg
      661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      721 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagtaaa gccagaggag
      781 atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      841 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      901 agcgtcggta ttaagcgggg gagaattaga taaatgggaa aaaattcggt taaggccagg
      961 gggaaagaaa caatataaac taaaacatat agtatgggca agcagggagc tagaacgatt
     1021 cgcagttaat cctggccttt tagagacatc agaaggctgt agacaaatac tgggacagct
     1081 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata caatagcagt
     1141 cctctattgt gtgcatcaaa ggatagatgt aaaagacacc aaggaagcct tagataagat
     1201 agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa gcagcagctg acacaggaaa
     1261 caacagccag gtcagccaaa attaccctat agtgcagaac ctccaggggc aaatggtaca
     1321 tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt
     1381 cagcccagaa gtaataccca tgttttcagc attatcagaa ggagccaccc cacaagattt
     1441 aaataccatg ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac
     1501 catcaatgag gaagctgcag aatgggatag attgcatcca gtgcatgcag ggcctattgc
     1561 accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca
     1621 ggaacaaata ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag
     1681 atggataatc ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga
     1741 cataagacaa ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct
     1801 aagagccgag caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca
     1861 aaatgcgaac ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga
     1921 agaaatgatg acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc
     1981 tgaagcaatg agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag
     2041 gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa
     2101 ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa
     2161 agattgtact gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag
     2221 gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag
     2281 gtttggggaa gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta
     2341 tcctttagct tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg
     2401 gggcaattaa aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg
     2461 aatttgccag gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta
     2521 agacagtatg atcagatact catagaaatc tgcggacata aagctatagg tacagtatta
     2581 gtaggaccta cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact
     2641 ttaaattttc ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat
     2701 ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt
     2761 tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat
     2821 actccagtat ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc
     2881 agagaactta ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct
     2941 gcagggttaa aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca
     3001 gttcccttag ataaagactt caggaagtat actgcattta ccatacctag tataaacaat
     3061 gagacaccag ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca
     3121 gcaatattcc agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac
     3181 atagtcatct atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag
     3241 catagaacaa aaatagagga actgagacaa catctgttga ggtggggatt taccacacca
     3301 gacaaaaaac atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat
     3361 aaatggacag tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata
     3421 cagaaattag tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg
     3481 caattatgta aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa
     3541 gaagcagagc tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg
     3601 tattatgacc catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg
     3661 acatatcaaa tttatcaaga gccatttaaa aatctgaaaa caggaaagta tgcaagaatg
     3721 aagggtgccc acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca
     3781 gaaagcatag taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca
     3841 tgggaagcat ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc
     3901 aatacccctc ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca
     3961 gaaactttct atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat
     4021 gtaactgaca gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact
     4081 gagttacaag caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca
     4141 gactcacaat atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta
     4201 gtcagtcaaa taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca
     4261 gcacacaaag gaattggagg aaatgaacaa gtagataaat tggtcagtgc tggaatcagg
     4321 aaagtactat ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt
     4381 aattggagag caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta
     4441 gccagctgtg ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc
     4501 ccaggaatat ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt
     4561 catgtagcca gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca
     4621 gcatacttcc tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat
     4681 ggcagcaatt tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag
     4741 gaatttggca ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa
     4801 ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa
     4861 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4921 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4981 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga
     5041 ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata
     5101 aaagtagtgc caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt
     5161 gatgattgtg tggcaagtag acaggatgag gattaacaca tggaaaagat tagtaaaaca
     5221 ccatatgtat atttcaagga aagctaagga ctggttttat agacatcact atgaaagtac
     5281 taatccaaaa ataagttcag aagtacacat cccactaggg gatgctaaat tagtaataac
     5341 aacatattgg ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat
     5401 agaatggagg aaaaagagat atagcacaca agtagaccct gacctagcag accaactaat
     5461 tcatctgcac tattttgatt gtttttcaga atctgctata agaaatacca tattaggacg
     5521 tatagttagt cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacagta
     5581 cttggcacta gcagcattaa taaaaccaaa acagataaag ccacctttgc ctagtgttag
     5641 gaaactgaca gaggacagat ggaacaagcc ccagaagacc aagggccaca gagggagcca
     5701 tacaatgaat ggacactaga gcttttagag gaacttaaga gtgaagctgt tagacatttt
     5761 cctaggatat ggctccataa cttaggacaa catatctatg aaacttacgg ggatacttgg
     5821 gcaggagtgg aagccataat aagaattctg caacaactgc tgtttatcca tttcagaatt
     5881 gggtgtcgac atagcagaat aggcgttact cgacagagga gagcaagaaa tggagccagt
     5941 agatcctaga ctagagccct ggaagcatcc aggaagtcag cctaaaactg cttgtaccaa
     6001 ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa aagccttagg
     6061 catctcctac ggcaggaaga agcggagaca gcgacgaaga gctcatcaga acagtcagac
     6121 tcatcaggct tctctaagca agcagtaagt agtacatgta atgcaaccta taatagtagc
     6181 aatagtagca ttagtagtag caataataat agcaatagtt gtgtggtcca tagtaatcat
     6241 agaatatagg aaaatattaa gacaaagaaa aatagacagg ttaattgata gactaataga
     6301 aagagcagaa gacagtggca atgagagtga aggagaagta tcagcacttg tggagatggg
     6361 ggtggaaatg gggcaccatg ctccttggga tattgatgat ctgtagtgct acagaaaaat
     6421 tgtgggtcac cgtctattat ggggtacctg tgtggaagga agcaaccacc actctatttt
     6481 gtgcatcaga tgctaaagca tatatgatac agaggtacat aatgtttggg ccacacatgc
     6541 ctgtgtaccc acagacccca acccacaaga agtagtattg gtaaatgtga cagaaaattt
     6601 taacatgtgg aaaaatgaca tggtagaaca gatgcatgag gatataatca gtttatggga
     6661 tcaaagccta aagccatgtg taaaattaac cccactctgt gttagtttaa agtgcactga
     6721 tttgaagaat gatactaata ccaatagtag tagcgggaga atgataatgg agaaaggaga
     6781 gataaaaaac tgctctttca atatcagcac aagcataaga gataaggtgc agaaagaata
     6841 tgcattcttt tataaacttg atatagtacc aatagataat accagctata ggttgataag
     6901 ttgtaacacc tcagtcatta cacaggcctg tccaaaggta tcctttgagc caattcccat
     6961 acattattgt gccccggctg gttttgcgat tctaaaatgt aataataaga cgttcaatgg
     7021 aacaggacca tgtacaaatg tcagcacagt acaatgtaca catggaatca ggccagtagt
     7081 atcaactcaa ctgctgttaa atggcagtct agcagaagaa gatgtagtaa ttagatctgc
     7141 caatttcaca gacaatgcta aaaccataat agtacagctg aacacatctg tagaaattaa
     7201 ttgtacaaga cccaacaaca atacaagaaa aagtatccgt atccagaggg gaccagggag
     7261 agcatttgtt acaataggaa aaataggaaa tatgagacaa gcacattgta acattagtag
     7321 agcaaaatgg aatgccactt taaaacagat agctagcaaa ttaagagaac aatttggaaa
     7381 taataaaaca ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag
     7441 ttttaattgt ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg
     7501 gtttaatagt acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac
     7561 actcccatgc agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta
     7621 tgcccctccc atcagtggac aaattagatg ttcatcaaat attactgggc tgctattaac
     7681 aagagatggt ggtaataaca acaatgggtc cgagatcttc agacctggag gaggcgatat
     7741 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7801 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7861 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7921 gacccttacc gtgcaagctc gccagctgct gtctggtata gtgcagcagc agaacaacct
     7981 cctccgcgca atcgaagccc agcagcatct gttgcaactc acagtctggg gcatcaaaca
     8041 gctccaggca agaatccttg ccgttgagag gtatctgaaa gaccagcaac ttcttgggat
     8101 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8161 taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat
     8221 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8281 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8341 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8401 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8461 atcgtttcag acccacctcc caatcccgag gggacccgac aggcccgaag gaatagaaga
     8521 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ccttagcact
     8581 tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact
     8641 cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata
     8701 ttggtggaat ctcctacagt attggagtca ggaactaaag aatagtgctg ttaacttgct
     8761 caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag tattacaagc
     8821 agcttataga gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt
     8881 gctataagat gggtggcaag tggtcaaaaa gtagtgtgat tggatggcct gctgtaaggg
     8941 aaagaatgag acgagctgag ccagcagcag atggggtggg agcagtatct cgagtccgtg
     9001 gggtgcgagg ctaagcactg cacagaggat agcttgctgt tggcatcctg tggaaggcac
     9061 gtctgattgc atgaaggttc agtgtcctag ttcccttccc ccaggaaaaa cgacacggga
     9121 gctggccaag acctctctgg gtgatgagcc taagggatgg ttttgtgtag ggcccctatg
     9181 cttggcggct ggggatcaga cctctacctt cacccatgag gtatcgatac cgcggggatc
     9241 ctctagagta gccacctccc ctgcgagcta agctggacag ccaatgacgg gtaagagagt
     9301 gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa tccaaagacg
     9361 ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc cgagggattt
     9421 ggagctcctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctaac
     9481 aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt tccagtcaca
     9541 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9601 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9661 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     9721 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9781 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9841 ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9901 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgaca tcgagcttgc
     9961 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    10021 tggcgagccc tcagatgctg catataagca gctgcttttt gcctgtactg ggtctctctg
    10081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca ggaaacaaat
    10261 aataaaaaag ccggattaat aatctggctt tttatattct ctctctagta tataaacgca
    10321 gaaaggccca cccgaaggtg agccagtgtg aatcgattcc tagggtacgg gttttgctgc
    10381 ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag aatcgcagat ccggcttcag
    10441 ccggtttgcc ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg
    10501 aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt
    10561 caggctgtct atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg
    10621 ttctagttgc tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc
    10681 tgttacattg tcgatctgtt catggtgaac agctttgaat gcaccaaaaa ctcgtaaaag
    10741 ctctgatgta tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt
    10801 tgatatgtaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat
    10861 agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg
    10921 tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca
    10981 tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt
    11041 gtagtgtttt tcttagtccg ttatgtaggt aggaatctga tgtaatggtt gttggtattt
    11101 tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat
    11161 ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt
    11221 tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc
    11281 ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa
    11341 tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa
    11401 tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt
    11461 tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc
    11521 ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc
    11581 tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat
    11641 tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt
    11701 ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct
    11761 tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa
    11821 ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac caattgagat
    11881 gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct
    11941 gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac
    12001 ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat
    12061 aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc
    12121 cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa
    12181 accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg
    12241 tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc
    12301 acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca
    12361 aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg
    12421 ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt
    12481 ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag
    12541 taaggcagcg gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc
    12601 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
    12661 ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct
    12721 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
    12781 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
    12841 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
    12901 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
    12961 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
    13021 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
    13081 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
    13141 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
    13201 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
    13261 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
    13321 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
    13381 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
    13441 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
    13501 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
    13561 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
    13621 ttaccagagg gcgccccagc tggcaattcc gacgtcaccg gt
//
