LOCUS       sequence-195163-       11666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of IKZF1 variant 1 with Q146H mutation and 
            FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1000
                     /label=chicken beta-actin promoter
     intron          1001..2009
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1933..1955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2017..2036
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2125..2149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3707..3731)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3751..3774
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3809..4358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3953..3970)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4180..4199
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4361..4363,4364..4366,4367..5080)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4361..4363,4364..4366,4367..5077)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4406..4427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4667..4686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5014..5035
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5157..5745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5210..5230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5628..5639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5748..5764)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5749..5765)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5954..6187
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6259..6380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6296..6315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6350..6369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6420..6555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6575..6594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6576..6594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6617..6975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6657..6678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6869..6888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7002..7106
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7108..7465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7108..7299
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7131..7151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7316..7451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7500..8294
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7500..8294
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7554..7573)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8164..8183
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8482..8501)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8526..8573
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8902..9490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9391..9410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9665..9968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9970..10168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10123..10143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10186..10366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10413..10538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11035..11268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11453..11497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc
     1021 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     1081 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     1141 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     1201 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     1261 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1321 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1381 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1441 tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac
     1501 ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc
     1561 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1621 tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1681 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1741 ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg
     1801 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1861 ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac
     1921 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1981 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2041 ctcatcattt tggcaaagaa ttcccctagc cgcggaatct agactgccgg atcgaattgt
     2101 cgacgaattc ggatctggcc aatcacaagt ttgtacaaaa aagttggcat ggatgctgat
     2161 gagggtcaag acatgtccca agtttcaggg aaggaaagcc cccctgtaag cgatactcca
     2221 gatgagggcg atgagcccat gccgatcccc gaggacctct ccaccacctc gggaggacag
     2281 caaagctcca agagtgacag agttgtggcc agtaatgtta aagtagagac tcagagtgat
     2341 gaagagaatg ggcgtgcctg tgaaatgaat ggggaagaat gtgcggagga tttacgaatg
     2401 cttgatgcct cgggagagaa aatgaatggc tcccacaggg accaaggcag ctcggctttg
     2461 tcgggagttg gaggcattcg acttcctaac ggaaaactaa agtgtgatat ctgtgggatc
     2521 atttgcatcg ggcccaatgt gctcatggtt cacaaaagaa gccacactgg agaacggccc
     2581 ttccattgca atcagtgcgg ggcctcattc acccagaagg gcaacctgct ccggcacatc
     2641 aagctgcatt ccggggagaa gcccttcaaa tgccacctct gcaactacgc ctgccgccgg
     2701 agggacgccc tcactggcca cctgaggacg cactccgttg gtaaacctca caaatgtgga
     2761 tattgtggcc gaagctataa acagcgaagc tctttagagg aacataaaga gcgctgccac
     2821 aactacttgg aaagcatggg ccttccgggc acactgtacc cagtcattaa agaagaaact
     2881 aatcacagtg aaatggcaga agacctgtgc aagataggat cagagagatc tctcgtgctg
     2941 gacagactag caagtaacgt cgccaaacgt aagagctcta tgcctcagaa atttcttggg
     3001 gacaagggcc tgtccgacac gccctacgac agcagcgcca gctacgagaa ggagaacgaa
     3061 atgatgaagt cccacgtgat ggaccaagcc atcaacaacg ccatcaacta cctgggggcc
     3121 gagtccctgc gcccgctggt gcagacgccc ccgggcggtt ccgaggtggt cccggtcatc
     3181 agcccgatgt accagctgca caagccgctc gcggagggca ccccgcgctc caaccactcg
     3241 gcccaggaca gcgccgtgga gaacctgctg ctgctctcca aggccaagtt ggtgccctcg
     3301 gagcgcgagg cgtccccgag caacagctgc caagactcca cggacaccga gagcaacaac
     3361 gaggagcagc gcagcggtct catctacctg accaaccaca tcgccccgca cgcgcgcaac
     3421 gggctgtcgc tcaaggagga gcaccgcgcc tacgacctgc tgcgcgccgc ctccgagaac
     3481 tcgcaggacg cgctccgcgt ggtcagcacc agcggggagc agatgaaggt gtacaagtgc
     3541 gaacactgcc gggtgctctt cctggatcac gtcatgtaca ccatccacat gggctgccac
     3601 ggcttccgtg atccttttga gtgcaacatg tgcggctacc acagccagga ccggtacgag
     3661 ttctcgtcgc acataacgcg aggggagcac cgcttccaca tgagctaccc aactttcttg
     3721 tacaaagtgg tgggaggagg tggatctggc gactacaagg acgacgatga caagtgactc
     3781 gagcgggatc aattccgccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     3841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     3901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4321 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggtgagca agggcgagga
     4381 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4441 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4501 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4561 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4621 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4681 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4741 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4801 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4861 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4921 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4981 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5041 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt
     5101 cgacctgcag gcatgcaagc ttcaggtagc cggctaacgt tatcaagctt atcgataatc
     5161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5761 ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5821 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5881 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5941 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6181 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6241 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6361 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6541 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attagcatgt
     6601 gagcaaaagg ccagcataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6661 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6721 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6781 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6841 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6901 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6961 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7021 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7081 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     7141 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7201 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7261 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7321 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7381 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7441 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     7501 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7561 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7621 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7681 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7741 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7801 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7861 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7921 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7981 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     8041 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8101 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8161 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8221 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8281 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     8341 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8461 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     8521 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     8581 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     8641 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8701 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     9601 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     9661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     9721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     9781 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     9841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     9901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     9961 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    10021 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    10081 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    10141 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
    10201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    10381 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    10441 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    10501 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    10561 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    10621 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    10681 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10741 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    10801 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    10861 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10921 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10981 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    11041 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    11101 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11161 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11221 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11281 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11341 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11401 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11461 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11521 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11581 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11641 tcagacccac ctcccaaccc cgaggg
//
