LOCUS       sequence-195165-       10416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of IKZF1 variant 1 with Q146H mutation and 
            FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1694..1718)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1738..1761
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1796..2345
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1940..1957)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2167..2186
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2348..2350,2351..2353,2354..3067)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2348..2350,2351..2353,2354..3064)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2393..2414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2654..2673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3001..3022
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3144..3732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3197..3217)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3615..3626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3735..3751)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3736..3752)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3941..4174
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4246..4367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4283..4302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4337..4356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4407..4542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4562..4581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4563..4581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4604..4962)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4644..4665)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4856..4875
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4989..5093
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5095..5452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5095..5286
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5118..5138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5303..5438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5487..6281
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5487..6281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5541..5560)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6151..6170
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6469..6488)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6513..6560
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6889..7477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7378..7397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7652..7955
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7957..8155
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8110..8130
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8173..8353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8400..8525
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9022..9255
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9440..9484
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9782..9899
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9997..10376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ccctagccgc
       61 ggaatctaga ctgccggatc gaattgtcga cgaattcgga tctggccaat cacaagtttg
      121 tacaaaaaag ttggcatgga tgctgatgag ggtcaagaca tgtcccaagt ttcagggaag
      181 gaaagccccc ctgtaagcga tactccagat gagggcgatg agcccatgcc gatccccgag
      241 gacctctcca ccacctcggg aggacagcaa agctccaaga gtgacagagt tgtggccagt
      301 aatgttaaag tagagactca gagtgatgaa gagaatgggc gtgcctgtga aatgaatggg
      361 gaagaatgtg cggaggattt acgaatgctt gatgcctcgg gagagaaaat gaatggctcc
      421 cacagggacc aaggcagctc ggctttgtcg ggagttggag gcattcgact tcctaacgga
      481 aaactaaagt gtgatatctg tgggatcatt tgcatcgggc ccaatgtgct catggttcac
      541 aaaagaagcc acactggaga acggcccttc cattgcaatc agtgcggggc ctcattcacc
      601 cagaagggca acctgctccg gcacatcaag ctgcattccg gggagaagcc cttcaaatgc
      661 cacctctgca actacgcctg ccgccggagg gacgccctca ctggccacct gaggacgcac
      721 tccgttggta aacctcacaa atgtggatat tgtggccgaa gctataaaca gcgaagctct
      781 ttagaggaac ataaagagcg ctgccacaac tacttggaaa gcatgggcct tccgggcaca
      841 ctgtacccag tcattaaaga agaaactaat cacagtgaaa tggcagaaga cctgtgcaag
      901 ataggatcag agagatctct cgtgctggac agactagcaa gtaacgtcgc caaacgtaag
      961 agctctatgc ctcagaaatt tcttggggac aagggcctgt ccgacacgcc ctacgacagc
     1021 agcgccagct acgagaagga gaacgaaatg atgaagtccc acgtgatgga ccaagccatc
     1081 aacaacgcca tcaactacct gggggccgag tccctgcgcc cgctggtgca gacgcccccg
     1141 ggcggttccg aggtggtccc ggtcatcagc ccgatgtacc agctgcacaa gccgctcgcg
     1201 gagggcaccc cgcgctccaa ccactcggcc caggacagcg ccgtggagaa cctgctgctg
     1261 ctctccaagg ccaagttggt gccctcggag cgcgaggcgt ccccgagcaa cagctgccaa
     1321 gactccacgg acaccgagag caacaacgag gagcagcgca gcggtctcat ctacctgacc
     1381 aaccacatcg ccccgcacgc gcgcaacggg ctgtcgctca aggaggagca ccgcgcctac
     1441 gacctgctgc gcgccgcctc cgagaactcg caggacgcgc tccgcgtggt cagcaccagc
     1501 ggggagcaga tgaaggtgta caagtgcgaa cactgccggg tgctcttcct ggatcacgtc
     1561 atgtacacca tccacatggg ctgccacggc ttccgtgatc cttttgagtg caacatgtgc
     1621 ggctaccaca gccaggaccg gtacgagttc tcgtcgcaca taacgcgagg ggagcaccgc
     1681 ttccacatga gctacccaac tttcttgtac aaagtggtgg gaggaggtgg atctggcgac
     1741 tacaaggacg acgatgacaa gtgactcgag cgggatcaat tccgcccccc ccctaacgtt
     1801 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1861 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1921 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1981 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     2041 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     2101 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     2161 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     2221 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     2281 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     2341 taataccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2401 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2461 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2521 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2581 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2641 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2701 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2761 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2821 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2881 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2941 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3001 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3061 caagtaaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttc aggtagccgg
     3121 ctaacgttat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     3181 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3241 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3301 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3361 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3421 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3481 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3541 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3601 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3661 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3721 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc
     3781 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     3841 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     3901 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3961 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4021 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4081 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4141 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     4201 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     4261 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4321 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     4381 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     4561 gccctatagt gagtcgtatt agcatgtgag caaaaggcca gcataaatca aaagaataga
     4621 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4681 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     4741 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     4801 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     4861 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     4921 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     4981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     5101 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5221 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5281 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5341 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5401 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     5461 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5521 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5581 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5641 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     5701 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5761 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5821 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5881 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5941 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6001 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6061 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6121 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6181 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6241 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6301 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6361 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6421 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     6481 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     6541 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6601 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6661 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     6721 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6781 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6841 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6901 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6961 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7021 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7081 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7141 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7201 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7261 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7321 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7381 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7441 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7501 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7561 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
     7621 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     7681 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     7741 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     7801 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     7861 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     7921 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     7981 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     8041 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     8101 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     8161 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8221 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8281 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8341 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8401 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8461 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8521 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8581 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8641 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8701 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8761 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     8821 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     8881 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8941 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9001 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9061 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9121 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9181 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9241 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9301 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9361 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9421 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9481 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9541 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9601 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9661 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9721 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     9781 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9841 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9901 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagccct
     9961 agatttaaat cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    10021 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    10081 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    10141 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    10201 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
    10261 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
    10321 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
    10381 aggtgagccc cacgttctgc ttcactctcc ccatct
//
