LOCUS       sequence-195173-       11072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of IKZF2 variant 2 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur
FEATURES             Location/Qualifiers
     source          1..11072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..1217
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1054..1073
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1265..1289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2790..2814)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2861..2950
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        2992..3491
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3027..3050)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3424..3443
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3512..4111
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3512..3531)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4008..4028
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4142..4175)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4231..4819
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4284..4304)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4702..4713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4822..4838)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4823..4839)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5028..5261
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5333..5454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5370..5389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5424..5443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5494..5629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5649..5668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5650..5668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5691..6049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5731..5752)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5943..5962
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6076..6180
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6182..6539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6182..6373
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6205..6225)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6390..6525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6574..7368
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6574..7368
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6628..6647)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7238..7257
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7556..7575)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7600..7647
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7976..8564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8465..8484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8739..9042
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9044..9242
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9197..9217
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9260..9440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9487..9612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10109..10342
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10527..10571
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10869..10986
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
       61 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
      121 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
      181 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
      241 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
      301 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
      361 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
      421 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
      481 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
      541 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
      601 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
      661 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
      721 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
      781 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
      841 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
      901 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
      961 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     1021 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     1081 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     1141 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     1201 tggctttttt gttagacgct agactgccgg atcgaattgt cgacgaattc ggatctggcc
     1261 aatcacaagt ttgtacaaaa aagttggcat ggaaacagag gccattgatg gctatataac
     1321 gtgtgacaat gagctttcac ccgaaaggga gcactccaat atggcaattg acctcacctc
     1381 aagcacaccc aatggacagc atgcctcacc aagtcacatg acaagcacaa attcagtaaa
     1441 gctagaaatg cagagtgatg aagagtgtga caggaaaccc ctgagccgtg aagatgagat
     1501 caggggccat gatgagggta gcagcctaga agaaccccta attgagagca gcgaggtggc
     1561 tgacaacagg aaagtccagg agcttcaagg cgagggagga atccggcttc cgaatggtga
     1621 acgccccttc cactgtaacc agtgtggagc ttcttttact cagaagggca accttctgag
     1681 acacataaag ttacactctg gagagaagcc gttcaaatgt cctttctgta gctacgcctg
     1741 tagaagaagg gacgccctca caggacacct caggacccat tctgtgggta aacctcacaa
     1801 gtgcaactac tgtggacgaa gctacaagca gcgcagttca ctggaggagc acaaggaacg
     1861 ctgccacaac tatctccaga atgtcagcat ggaggctgct gggcaggtca tgagtcacca
     1921 tgtacctcct atggaagatt gtaaggaaca agagcctatt atggacaaca atatttctct
     1981 ggtgcctttt gagagacctg ctgtcataga gaagctcacg gggaatatgg gaaaacgtaa
     2041 aagctccact ccacaaaagt ttgtggggga aaagctcatg cgattcagct acccagatat
     2101 tcactttgat atgaacttaa catatgagaa ggaggctgag ctgatgcagt ctcatatgat
     2161 ggaccaagcc atcaacaatg caatcaccta ccttggagct gaggcccttc accctctgat
     2221 gcagcacccg ccaagcacaa tcgctgaagt ggccccagtt ataagctcag cttattctca
     2281 ggtctatcat ccaaatagga tagaaagacc cattagcagg gaaactgctg atagtcatga
     2341 aaacaacatg gatggcccca tctctctcat cagaccaaag agtcgacccc aggaaagaga
     2401 ggcctctccc agcaatagct gcctggattc cactgactca gaaagcagcc atgatgacca
     2461 ccagtcctac caaggacacc ctgccttaaa tcccaagagg aaacaaagcc cagcttacat
     2521 gaaggaggat gtcaaagctt tggatactac caaggctcct aagggctctc tgaaggacat
     2581 ctacaaggtc ttcaatggag aaggagaaca gattagggcc ttcaagtgtg agcactgccg
     2641 agtccttttc ctagaccatg tcatgtacac cattcacatg ggttgccatg gctaccggga
     2701 cccactggaa tgcaacatct gtggctacag aagccaggac cgttatgagt tttcatcaca
     2761 cattgttcga ggggagcaca cattccacta cccaactttc ttgtacaaag tggtgggtgg
     2821 aggcggttca ggtggcggag gaagcggagg cggtggaagt tacccctacg acgtgcccga
     2881 ctacgccggc tatccgtatg atgtcccgga ctatgcagga tcctatccat acgacgttcc
     2941 agattacgct tagttaatta aggccggccc tcgaggttaa cgaattctac cgggtagggg
     3001 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     3061 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3121 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3181 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3241 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3301 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     3361 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     3421 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     3481 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     3541 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3601 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3661 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3721 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3781 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3841 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3901 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3961 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4021 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4081 ctggtgcatg acccgcaagc ccggtgcctg atgtacaagt aggaattcgt cgagggacct
     4141 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     4201 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4261 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4321 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4381 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4441 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4501 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4561 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4621 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4681 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4741 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4801 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     4861 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4921 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4981 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5041 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5221 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5281 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5461 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5641 ccaattcgcc ctatagtgag tcgtattagc atgtgagcaa aaggccagca taaatcaaaa
     5701 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5761 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5821 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5881 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5941 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6001 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     6061 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6121 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6181 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     6541 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6601 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6661 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6721 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6781 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6841 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6901 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6961 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7021 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7081 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     7141 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7201 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7261 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7321 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7381 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7441 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7501 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     7561 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     7621 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     7681 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     7741 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     7801 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     7861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8101 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8161 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8221 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8281 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8341 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8401 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8461 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8521 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8581 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8641 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
     8701 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8761 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8821 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8881 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8941 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     9001 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     9061 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9121 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9181 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     9241 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9421 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9481 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9541 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9601 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9661 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9721 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9781 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9841 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9901 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9961 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    10021 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10081 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10141 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10201 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10261 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10321 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10381 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10441 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10501 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10561 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10621 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10681 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10741 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10801 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
    10861 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10921 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10981 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
    11041 tagccctaga tttaacccaa cagaaggctc ga
//
