LOCUS       sequence-195175-       10444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of RFP-RabG3B-DN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10444)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBI10:RFP-RabG3B-DN
FEATURES             Location/Qualifiers
     source          1..10444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     526..545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     779..796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    811..951
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(877..896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1037..1059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1295..1489
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1555..2628)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3057..3686)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5017..5041
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    5206..5227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5242..5247,5248..5265,5266..5272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5280..5296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5285..5307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5304..5320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5304..5320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6008..6682
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6008..6682
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     6406..6425
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6600..6623
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    6695..6719
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7368..7392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7762..7941
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7983..8534
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8554..8806
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8899..8923
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9444..10235
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
       61 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      781 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
      841 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      901 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
      961 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1021 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     1081 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     1141 tccgcgccct ggtagattgc ctggccctag gccagccatt tttgagcggc cagcggccgc
     1201 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     1261 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     1321 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     1381 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     1441 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     1501 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     1561 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     1621 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     1681 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     1741 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     1801 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     1861 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     1921 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     1981 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     2041 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     2101 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     2161 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     2221 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     2281 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     2341 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     2401 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     2461 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     2521 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     2581 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     2641 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     2701 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     2761 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     2821 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     2881 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     2941 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     3001 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     3061 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     3121 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     3181 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     3241 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     3301 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     3361 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     3421 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     3481 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     3541 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     3601 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     3661 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     3721 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     3781 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     3841 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     3901 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     3961 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
     4021 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
     4081 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
     4141 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
     4201 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
     4261 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
     4321 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
     4381 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
     4441 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
     4501 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
     4561 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
     4621 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
     4681 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
     4741 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
     4801 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     4861 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
     4921 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
     4981 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
     5041 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
     5101 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5161 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5221 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5281 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
     5341 ggtacccgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc
     5401 gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca
     5461 aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc
     5521 ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat
     5581 aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt
     5641 aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct
     5701 ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag
     5761 attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa
     5821 ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga
     5881 ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg
     5941 atcgaatttg tagattaatc tgagtttttc tgattaacac tcgagtgcgg gatcctctag
     6001 aactagtatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
     6061 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6121 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6181 ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga
     6241 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6301 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     6361 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     6421 gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa
     6481 gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
     6541 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa
     6601 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
     6661 gggccgccac tccaccggcg cccaattgtt aatcacaagt ttgtacaaaa aagcaggctc
     6721 cgcggccgcc cccttcacca tgtcgacgcg aagacgaact ttactcaaag ttataattct
     6781 tggagacagc ggggttggca aaaactcgtt gatgaatcaa tatgtgaata acaagtttag
     6841 tcaacagtac aaagctacga tcggagctga ttttgtcact aaggagcttc aaattgatga
     6901 caggcttgtc acattgcaaa tatgggacac tgctgggcaa gagaggtttc aaagtcttgg
     6961 tgttgctttc tatagaggtg cagattgttg tgttcttgtc tatgatgtga atcacttgaa
     7021 gtcatttgaa tctctcgaca attggcacaa cgagtttctt acacgggcta gtccacgtga
     7081 cccaatggca ttccctttta tacttcttgg taataaggtt gatattgatg gaggaaatag
     7141 ccgagtggta tctgagaaga aggctagaga atggtgtgct gaaaagggaa acatagtcta
     7201 tttcgagaca tcggctaaag aagattacaa tgtcgatgac tccttcttgt gcatcacaaa
     7261 acttgccctt gcaaatgaac gcgaccaaga tatatatttc cagccagata ctggttcggt
     7321 gcctgagcaa agaggaggtt gtgcttgctg aaagggtggg cgcgccgacc cagctttctt
     7381 gtacaaagtg gtgattaagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta
     7441 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     7501 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     7561 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     7621 gacctgcagg catgcgacgt cgggccctct agaggatccc cgggtaccgc gaattatcga
     7681 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     7741 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc
     7801 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     7861 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     7921 attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg aggggatcta
     7981 ccatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg
     8041 cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc
     8101 cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc
     8161 tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta cgcgggtccc tggaaggcac
     8221 gcaacgccta cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga
     8281 cgggactggg ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca
     8341 agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc
     8401 tcggatatgc cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg
     8461 acgtgggttt ctggcagctg gacttcagcc tgccggtgcc gccccgtccg gtcctgcccg
     8521 tcaccgaaat ctgatgaccc ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
     8581 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     8641 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     8701 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     8761 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc ggcatgcaag
     8821 ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     8881 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     8941 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     9001 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     9061 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     9121 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     9181 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     9241 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     9301 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     9361 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     9421 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     9481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     9541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     9601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     9661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     9721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     9781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     9841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     9901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     9961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
    10021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
    10081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
    10141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
    10201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
    10261 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
    10321 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
    10381 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10441 actg
//
