LOCUS       sequence-195177-        3414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A0LWB3
FEATURES             Location/Qualifiers
     source          1..3414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggggggtt cagaggtcgg taccgtgccg gtaacctggc ggctcggcgt ggacgtgggg
       61 gaaagatcaa tcgggcttgc tgccgtgtca tatgaagagg acaagcctaa ggaaatcttg
      121 gctgcagtgt cctggatcca tgatgggggg gttggcgatg aacggtccgg ggcaagtcga
      181 cttgccctcc gaggcatggc aagaagagcc cgaaggctgc gccggtttag gagagcccgc
      241 ctccgcgacc tggacatgtt gctgagtgag ttgggatgga cccccctccc tgacaaaaac
      301 gtctcaccag ttgatgcctg gctggcacgc aaaagactgg ccgaggaata tgtggtggat
      361 gaaactgaga ggcgaagact gctgggctac gccgtgtctc atatggcccg gcaccgaggg
      421 tggcgcaatc catggactac gattaaggac ctgaaaaatc tgccacagcc ctcagactca
      481 tgggagcgga ctagagagtc actggaagcc aggtatagcg tgtctctgga gcccggcact
      541 gtcgggcaat gggcaggcta tcttctgcag agagcaccgg gcataagact taatcccaca
      601 caacagtccg ccggtcgaag ggccgagttg agtaacgcaa cagcctttga gacaagactg
      661 cgacaagaag acgtcctttg ggaactgaga tgcatcgccg atgtgcaagg gttgcccgag
      721 gacgtcgtta gcaacgttat agacgcagtg ttttgccaga aacggccctc cgtaccagcg
      781 gaaagaatcg gcagagatcc gctggacccc agccagcttc gcgctagcag agcctgtctg
      841 gagtttcaag agtatcggat tgtggcagcc gtcgcgaatc tgagaattag agatggcagc
      901 ggtagtcggc cactgtcttt ggaggagcgg aatgccgtta tagaggctct gcttgcgcag
      961 accgaaaggt ctttgacgtg gagcgacatt gcgctggaaa ttctgaaact gcctaacgaa
     1021 tccgacctga cctctgtccc ggaggaagac gggcctagct ccctggccta ctctcaattt
     1081 gctccgttcg atgagacttc agcccgaatc gcggaattca ttgcaaaaaa ccggcgcaag
     1141 atccccacct ttgctcagtg gtggcaagag caagatcgga caagccgcag tgacctggtg
     1201 gctgctctgg cagataactc aatcgctggc gaggaagaac aggagctgct tgtccacctg
     1261 ccggacgctg agctcgaggc ccttgaaggg ctcgccttgc ctagcggccg ggtggcctac
     1321 tcccgcctga cgctgtccgg acttaccagg gtgatgagag acgatggagt ggacgttcac
     1381 aacgctcgca agacttgctt tggagttgac gacaactggc gccccccgct gcctgccctg
     1441 cacgaagcta ccggacatcc cgtggtcgat cggaatttgg cgatcctgcg gaagtttctc
     1501 tcatccgcta ccatgaggtg gggaccacct caatctatcg tggtggagct ggcccggggc
     1561 gccagcgagt ctcgagagcg acaggccgag gaggaagccg ccagaagagc ccatcggaaa
     1621 gccaacgatc gaataagagc cgagctgcgg gcatccggac tcagcgatcc tagccctgct
     1681 gaccttgtcc gcgcaagact gctggaactg tatgactgcc actgcatgta ttgtggggca
     1741 cctataagct gggagaactc tgaactggac cacatcgtcc cacggactga tggcggatca
     1801 aatcgccacg agaatctggc cattacttgc ggcgcatgta acaaggagaa aggtcgacgg
     1861 ccgtttgcga gttgggccga gacgtcaaat agggtgcagc tgagggatgt catcgatcgc
     1921 gtgcagaaac tcaaatactc aggaaatatg tattggacca gagacgaatt tagcagatac
     1981 aaaaagtctg tggtagctag actcaagcga aggacgtcag accccgaggt catccagagt
     2041 attgagagta ccggctatgc cgcagtggcg ctccgagaca gactgctgag ctacggcgag
     2101 aaaaacgggg tggctcaggt cgcagtcttt cgcggaggcg ttacagctga agcgagacgc
     2161 tggcttgata tatccataga gaggctgttt tctagggtgg ccatttttgc gcagtccact
     2221 agcacaaaga ggctcgacag acgacatcat gccgtggatg ctgtggttct gacaacgctc
     2281 acacctggtg ttgcgaagac tttggctgat gcgcgcagca gacgagttag cgccgagttc
     2341 tggcggaggc ctagcgacgt caaccgacac agcactgagg agccgcagag cccggcctac
     2401 aggcaatgga aggaatcttg ttctggtctg ggcgacttgc tcatttccac tgctgcacga
     2461 gattccattg cagtagctgc acccttgagg ctccggccga ctggagcact gcacgaagaa
     2521 acccttaggg ccttcagtga acacacggtc ggggcagcct ggaaaggcgc agagctgcgc
     2581 aggattgtgg aacccgaagt atacgccgcc tttctggctt tgaccgaccc tggcggccgc
     2641 ttcctgaaag tgtcaccttc agaagatgtg ctgcccgcgg acgaaaacag acatatcgtg
     2701 ctgtcagata gagtcctggg gccacgggac agagtgaagc tgttcccaga cgaccgggga
     2761 agtataaggg tgcgaggggg tgcggcgtat attgctagct tccaccacgc tcgcgtgttt
     2821 aggtggggaa gctctcacag cccctcattt gccctgctgc gcgtcagtct tgcagatctt
     2881 gcggtagccg gactgttgag ggatggggtg gacgtcttca ctgccgaact ccctccttgg
     2941 acacctgcct ggaggtatgc gtcaatcgct ctggtcaaag cggtcgagag tggcgatgct
     3001 aaacaggttg gctggttggt ccctggcgac gagctggact tcgggcctga aggggtcacc
     3061 acagcggcgg gcgacctcag tatgttcttg aaatattttc cagagcgcca ctgggttgtt
     3121 acaggatttg aagacgataa gagaatcaac cttaagcccg ccttcctctc tgctgaacag
     3181 gcagaggtgc tgcgcaccga gcgcagtgac cggccggaca ccctgaccga ggctggagag
     3241 atactcgccc aatttttccc ccgctgttgg agagcaaccg tcgccaaggt gctctgtcac
     3301 ccgggactga cggttattag aagaacagcc cttggccagc caaggtggcg aaggggtcac
     3361 ctgccatata gctggcgtcc ctggtcagcc gacccatgga gcggtggaac ccca
//
