LOCUS       sequence-195179-        3303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage, also expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pCAG-B2UP49_GFP
FEATURES             Location/Qualifiers
     source          1..3303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcaagga gcctcacttt cagcttcgac atcggttacg cttccattgg gtgggccgtg
       61 attgctagtg cctcccatga cgacgccgat ccttctgtgt gtgggtgcgg aacggtgctg
      121 tttccaaagg atgactgtca ggcattcaaa aggagagagt atagaaggct gcgcaggaac
      181 atccgaagtc gcagagtccg gatcgagcgg atcgggagac tccttgtgca agctcagatc
      241 atcacacccg aaatgaaaga aacctccggc cacccagcac ctttttacct tgcaagcgag
      301 gccctgaaag ggcatcggac actggctcca attgaattgt ggcacgtgct taggtggtat
      361 gcacacaacc gagggtatga caacaacgcg tcttggtcta actctctctc agaagacgga
      421 ggtaacgggg aagatactga gcgcgtcaaa cacgctcagg atctgatgga caagcacgga
      481 accgcaacca tggccgaaac catctgtcgg gagcttaagc tggaggaggg aaaggcggat
      541 gcccccatgg aggtgagtac gccggcttac aaaaatctga atactgcgtt tcccaggctg
      601 atcgttgaga aggaggtgag acggattctc gagctgagtg cgcccctgat cccagggctg
      661 acagccgaga tcattgagct gatcgcccaa caccatcctt tgacgacaga acagcggggg
      721 gtgctgctgc agcacggtat caagctcgca cgaaggtatc gcggcagtct cctttttggc
      781 caactgatac cacggttcga caaccgaatt atcagcagat gccctgtaac atgggcgcag
      841 gtctatgagg ccgagctgaa gaaagggaat agcgagcagt ccgctagaga acgagctgaa
      901 aaattgtcta aggtccccac ggccaattgc cctgaatttt acgagtatcg catggcccgc
      961 attctgtgca acatccgggc cgacggtgaa cctctctctg cggaaatcag aagggagctg
     1021 atgaatcagg cacgccaaga ggggaaactg accaaggcct ccctggagaa agccataagt
     1081 tctagactgg ggaaggaaac tgaaaccaac gtcagcaatt acttcaccct tcatcccgac
     1141 tccgaagagg cactgtactt gaatcccgcc gttgaggtcc tgcagcgctc agggattggg
     1201 cagatacttt cacccagcgt gtacagaatt gctgccaatc gcctgagacg aggaaaaagt
     1261 gtgacaccca attacctgct caacctcctg aagtccaggg gtgagtcagg agaggccctg
     1321 gaaaagaaga ttgagaaaga gagtaagaag aaagaggcag attatgccga cactcctctg
     1381 aaacctaagt acgccaccgg ccgcgccccg tatgcacgca ccgttctcaa gaaggtggtc
     1441 gaggaaatac tggatgggga ggatcctacc cggccagcca ggggcgaggc tcaccctgac
     1501 ggggaattga aagcccacga tgggtgtctg tattgtcttc ttgataccga ctccagcgta
     1561 aatcagcacc agaaggagcg gcggctggat accatgacta ataaccatct cgtgagacac
     1621 aggatgctca tcctcgacag actgctcaaa gacctcattc aggactttgc cgatggacaa
     1681 aaggacagga tctccagggt gtgcgtcgaa gtcggcaaag aactgacaac attttcagcc
     1741 atggactcta agaagattca gagagagctc acgcttaggc agaagtcaca cactgacgct
     1801 gtcaatagac tcaagagaaa gctgcctgga aaagccctta gcgcaaatct gataaggaag
     1861 tgccggatcg ccatggacat gaactggacc tgccccttta cgggtgcgac gtacggagat
     1921 catgagctgg agaacctgga actggagcac atcgttcctc acagctttcg ccaatctaac
     1981 gcgctgtcat ccctggtgct cacctggcct ggcgtcaatc gcatgaaggg gcaacggaca
     2041 ggatacgact tcgttgagca ggaacaggaa aatcccgtgc cagataagcc caacctgcac
     2101 atttgttcct tgaataacta tcgcgaactg gtggagaaac tcgacgataa gaaagggcat
     2161 gaggacgaca gaaggcgcaa gaagaaacgg aaggcccttc ttatggtgag aggattgagc
     2221 cacaagcatc agtcacagaa tcatgaggct atgaaggaga ttggaatgac cgagggcatg
     2281 atgacacagt cctcacatct tatgaagctc gcttgtaaat caatcaagac atcactgccc
     2341 gacgcacata tcgatatgat ccccggcgcc gttaccgccg aggtgagaaa ggcctgggat
     2401 gtattcggag ttttcaagga gctctgtccc gaggccgcag atcctgatag cgggaagatc
     2461 ctcaaggaaa acctgcgctc acttacgcat ctgcatcacg cacttgatgc ctgcgtactg
     2521 gggctgatcc catatattat ccctgctcat cataatggtc ttctgcggcg agtgctggct
     2581 atgcgacgca tcccagagaa gctgataccc caggtgcggc ctgtcgcaaa tcagcggcat
     2641 tacgtgttga atgacgatgg acgaatgatg ctgagggacc tcagcgcctc cctgaaggag
     2701 aacatccgcg agcagctgat ggagcaacgg gtgatccagc atgtgccggc agacatgggc
     2761 ggagccctgc tgaaagaaac tatgcaaaga gtcttgtctg tggatggatc tggagaagac
     2821 gcaatggtaa gtctgtccaa gaagaaggac ggcaaaaagg agaagaacca ggtgaaggcc
     2881 tccaaattgg tcggggtgtt ccccgaaggg ccatcaaaac ttaaggcact taaggctgcc
     2941 atcgagatcg acggcaacta cggagtggca ttggatccga aacctgtcgt gatcagacac
     3001 atcaaagtgt ttaaaagaat catggcgctg aaggagcaga acggtggtaa gccggttagg
     3061 atcctgaaga agggaatgtt gattcacctg acctcctcaa aagatcctaa gcacgccggg
     3121 gtctggagaa ttgagtctat ccaggattca aagggggggg tcaagctcga cctgcagagg
     3181 gctcattgcg ccgtgccaaa aaataagaca cacgaatgca attggaggga ggtcgacctg
     3241 atctcactgc tcaaaaagta ccagatgaag aggtacccaa cttcatatac gggcacccca
     3301 aga
//
