LOCUS       sequence-195181-        3393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B9M9X8
FEATURES             Location/Qualifiers
     source          1..3393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcccaac atgtattcgg cctcgacatc gggattgctt ccgtgggatg ggctatcctg
       61 ggggaacaga ggataatcga tctcggcgtg cgatgttttg acaaggcaga gacggccaag
      121 gaaggcgatc ctttgaactt gaccaggagg caggcccgcc ttctgcgacg gcgcctgtac
      181 aggcgcgctt ggagattgac acagctcagc aggctcctga aacgaaaagg gctgattgcc
      241 gatgccaagc tcttcgccaa agctcctagc tatggtgaca gtgcgtggga gctgcgccga
      301 caggggctgg atcggcttct gacaccactg gaatgggccc gggtcatcta tcatcagtgt
      361 aaacatcgag ggttccattg gacgtctaaa gccgaggaag caaaggccga tagtgatgcc
      421 gagggaggtc gagttaagca aggtctggcc cataccaaag ccctcatgca ggcaaagaat
      481 tacagaagcg ccgctgagat ggtgctggcg gaattcccag acgcccagcg aaataagcga
      541 ggacagtatg acaaagcttt gagccgggtt ctgctggggg aagagctggc actcttgttc
      601 gcaacccaaa ggcgcctggg gaatccacac gccagtgatt ttttcgagaa actgattctc
      661 ggcgacggag ataggaaatc tgggctgttt tggcaacaga agcccgcact ctccggggcc
      721 gaccttctga aaatgttggg gaaatgtacc ttcgagaaag gcgagtaccg cgcccccaaa
      781 gcctcttttt ctgtcgagag acatgtttgg ctgacaaggc ttaacaattt gcggatagtt
      841 gtggatggac ggagccggcc tctgaatgaa gcagaacggc aggccgccct cctgctgccc
      901 tatcagactg agacgagtaa atacaagacg ctcaaaaacg cctttatcaa ggcaggcttg
      961 tggggtgatg gcgtgcgatt tggagggctg gcctacccaa gccaggcgca aatagacgcg
     1021 gaaaaaacta aggacccaga ggatcaattc ctggtcaagt tgccagcttg gcatgaactg
     1081 agaaaggctt tcaaggcagc aggtcacgag gccctgtggc agcagatcag cacgcccgcg
     1141 ctggacggag atccaacact cctggaccaa atagccactg tgctgagcgt ttacaaggac
     1201 ggtgctgaag tagtgcagca gctcagacaa ctggccctgc cagaacctgc tgcttctatc
     1261 gccgttctcg aaaagatttc attcgataag ttttccagct tgagcctgaa agctctgcgg
     1321 agaatagtcc cgctgatgca gagtgggctt cgatatgacg aggccgtcgc acaaattcca
     1381 gagtatggcc atcattctca acggattgaa cctggagcgg ccaaacacct ttacctgcca
     1441 ccattctacg aagctcagcg gaagtatgca ggcaaaggcg accatatcgg cagtatgcag
     1501 tttagggatg atgcagatat cccccgaaat cctgttgtct tgcgcgctct caatcaagca
     1561 agaaaggtgg tcaatgccct tattcgggag tacggttctc ctatcgctgt taacatagag
     1621 atggcacggg atctgtcccg ccctctggat gagcgaaaca aggtcaagcg cgcccaggag
     1681 gaatttaggg ataggaatga cagggctcgg agcgaatttg agagggattt tggatacaaa
     1741 cctaaggctg ccgcgttcga aaagtggatg ctgtataggg agcagctggg ccagtgcgcg
     1801 tactcccagc agccgctgga tatccagagg gtcttggacg atcacaatta tgcccaggtc
     1861 gaccacgctt tgccttacag tagatcatat gatgattcaa aaaataacaa agttctggtc
     1921 ctgactcacg aaaatcagaa caagggaaac cggacggcct ttgaatacct cacatcattt
     1981 cccgacggcg aggatggcga gaggtggagg acctttgtgg catgggtgca ggggaacaag
     2041 gcttaccgca tggccaaaag aaatcgcctt ctgcgcaaga actacggcgt ggacgagtcc
     2101 aaagggttca ttgacaggaa tctcaacgat accagataca tctgtaaatt ttttaagaac
     2161 tacgtggagg aacacctgca gctcgcagca agagcagacg gggatacagc ccgccgatgc
     2221 gtagtcgtta acgggcagct gacagcgttc ctgagagccc gatggggact tacaaaagtg
     2281 cgcggtgatt ccgatcgcca tcatgccttg gatgcagccg tggtggccgc ctgcactcat
     2341 ggaatggtca aagccctggc cgactacagt cggagaaaag agatcagctt cctgcaggag
     2401 ggcttccccg acccagagac tggagagatt ttgaaccctg cagcctttga ccgcgcccgc
     2461 cagcacttcc cagaaccctg gacacacttc gcacatgaat tgaaagctcg gctgttcacc
     2521 gatgatttgg cagccctgcg agaggatatg cagcgactgg gaagttacac cacggaagat
     2581 ctgggtcgcc tgcgaacctt gttcgtcagc agggcccctc agaggcgcag cgggggcgca
     2641 gtgcacaaag aaacaatata cgctcagcct gagagcctga agcaacaggg cggcgtgatt
     2701 gagaaaatcc tgcttacatc tctcaagctt caagatttcg acaaactgct gaacccagaa
     2761 tctaacgatc acttcgtgga gcctcacaga aacgagaggc tctatgccgc aatcaggcag
     2821 aggctcgaac agtttggtgg aagagcagat aaggcttttg gaccagacaa cttgtttcat
     2881 aaacctgata aaaataatca gcccaccgga cccgtggtaa ggagtataaa gctggttagg
     2941 ggcaaacaga cgggaatccc tattcggggg ggcttggcta agaacgactc catgctgcga
     3001 gttgatatct ttaccaaagc tggaaaattt catctggtgc cagtgtacgt tcaccatcgg
     3061 gtgacaggcc tgcccaaccg ggcaatcgta gcctttaagg acgaggatga gtggacactg
     3121 atcgacgaaa gcttcgcttt tctctttagc gtatatccca acgattatgt gaaggtgaca
     3181 ctgaaaaagg aacaacagtc aggatattac tcaggagctg ataggagcac tggcgccatg
     3241 aatctctggg cccacgaccg ggccgcaagt gttgggaaag atgggttgat ccgcggtata
     3301 ggggtgaaga cggccctgtc cgtggaaaaa tttaacgtcg atgtgctcgg ccgcatttat
     3361 ttggcgcccc cggagacacg gagcggtttg gcc
//
