LOCUS       sequence-195185-        3504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-D3NT09
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcaagac cagcgttccg cgccccgagg agggaacatg tcaatggttg gactcccgac
       61 cctcatagaa tcagtaagcc tttcttcatc ctggtcagct ggcacctgtt gtccagagtt
      121 gtcatcgaca gcagcagcgg ctgcttcccg gggacctcta gggaccacac tgacaagttc
      181 gcagaatggg aatgcgcagt tcagccctat aggctcagct tcgacctcgg tacaaattct
      241 attggctggg gtcttcttaa tctggacaga cagggcaaac ctcgagagat tagagccttg
      301 gggtccagaa tcttcagcga tggacgagat ccacaagaca aagcctcatt ggctgtggct
      361 cggcggctgg cccggcagat gaggcggagg cgggatcgat atctgacaag acgaacacgc
      421 ctgatgggtg ccttggtccg gttcggcctc atgccagcag acccggccgc aagaaaaaga
      481 ctcgaagtgg ccgtggaccc atatttggca agagaaagag ccacacgcga acggcttgaa
      541 ccctttgaga tcggcagagc tttgttccac ctgaatcaga ggcggggcta caagcctgtt
      601 agaaccgcta caaagcctga cgaggaggct ggcaaggtga aagaggccgt tgagaggctg
      661 gaagccgcca ttgccgccgc cggggcgccc accctcgggg cctggttcgc ttggagaaaa
      721 acccgaggcg agacacttag ggccaggctg gcaggcaagg ggaaagaggc agcgtatccc
      781 ttctaccccg cccgccgcat gcttgaagca gagttcgata ccctctgggc agagcaggca
      841 agacaccatc ccgatctcct gactgctgag gccagggaga tactgaggca ccggatattt
      901 caccaacgcc cgttgaagcc cccacctgta ggtagatgca ccttgtaccc agacgacggg
      961 agagccccca gagcactgcc ttctgcacaa aggctgagac tgttccagga gctggctagt
     1021 cttcgagtaa ttcatctgga cctttcagag aggccactga ctcctgccga acgggacaga
     1081 attgtagcct tcgtccaagg ccggccacca aaggccggtc ggaaaccagg aaaggtccag
     1141 aagagcgtgc ctttcgagaa gctccgagga ctgctggaac tgcccccggg cacaggattc
     1201 agcctggaga gcgataagcg accggagctg ctcggggatg aaacaggcgc acgcatcgct
     1261 cccgctttcg gccctggctg gaccgccctg cccttggagg agcaagacgc acttgtcgag
     1321 cttctcctca ccgaagccga gcccgagagg gcaatcgccg ccctcactgc ccgctgggca
     1381 cttgatgaag ctacagctgc caagcttgct ggagccacac tccccgattt ccacggacgg
     1441 tacggacggc gagctgtggc tgaactgctg ccagtcctgg aaagggaaac cagaggggac
     1501 cccgacggca gagttcgccc aatccgactg gacgaagccg tgaagctgct gcgcgggggg
     1561 aaggatcatt ccgacttcag ccgcgagggt gcacttctcg acgctctccc ctattacggc
     1621 gctgtgctgg agaggcacgt cgcatttgga actggcaacc cggccgatcc ggaggagaag
     1681 agagttggtc gcgtcgccaa ccccactgtt cacatcgccc ttaatcagct gaggcatttg
     1741 gtcaacgcca ttctggccag gcatggaagg cctgaggaaa tcgttatcga acttgcaagg
     1801 gacctgaaaa ggtccgcgga ggaccggagg cgcgaggaca aacgccaggc tgataatcag
     1861 aaaagaaacg aagaacggaa aaggctgatt ctgtccctgg gggaacggcc aaccccccga
     1921 aatctgctca aactccgcct gtgggaagag cagggccctg tggagaatag gcggtgccca
     1981 tattccggag agacaatctc aatgcgaatg ctcctgagcg aacaagtgga catcgatcac
     2041 atccttccct ttagcgtgtc tctggacgat tccgccgcga acaaggtcgt ttgcctccgc
     2101 gaagccaacc gcattaaacg gaaccggagc ccttgggagg cttttggaca cgactccgaa
     2161 aggtgggctg gaatcctggc ccgcgctgaa gcgctcccaa aaaataagag gtggaggttt
     2221 gcacccgatg ctctggagaa gcttgagggg gaaggaggct tgcgcgctcg gcacctcaat
     2281 gatactcgcc atctgtccag gctggcagtg gagtacctgc gctgtgtgtg tcccaaagtg
     2341 cgcgtctctc ccgggagact taccgccttg ttgaggcgca ggtggggaat cgatgctatt
     2401 ctcgctgagg cagacggccc accccctgag gtgcccgctg aaaccctcga tccatctccc
     2461 gctgaaaaga accgggcgga tcatagacat catgctctgg acgctgtggt gatcgggtgc
     2521 attgatagat caatggtcca gagggttcag ctcgctgctg cttccgctga gagagaggcc
     2581 gccgcacgcg aggataacat ccgcagagtg ctggaagggt tcaaagaaga accctgggac
     2641 gggttcaggg cggaactcga gcgaagggcc aggacgatcg tcgtttccca tcgcccggag
     2701 cacggcattg gtggtgcgct gcacaaagag actgcatatg ggcctgttga tccccctgaa
     2761 gagggcttta acctcgtggt gagaaaacca attgacggcc tgagtaaaga tgagatcaat
     2821 tccgtccgcg acccaaggct tcgcagagct ttgatcgacc gcctggccat ccgcagaagg
     2881 gatgcgaacg accctgccac agccctggca aaggccgcgg aagacctggc tgcccaaccc
     2941 gcctcccggg gaattaggag ggtgagagtg ctcaaaaaag agtctaaccc catacgggtg
     3001 gaacacgggg ggaatccttc tggtccacgg agcgggggac cctttcacaa actgctgctg
     3061 gctggcgagg tacaccacgt agatgtcgca ttgcgcgccg atggcaggag gtgggtaggc
     3121 cattgggtaa ccctgtttga agctcacgga ggtcgcggcg cggatggtgc tgctgcacct
     3181 ccccggctcg gcgatggaga acggtttctc atgcgcttgc ataagggcga ctgccttaaa
     3241 ttggaacaca agggaagagt tagagtgatg caggtggtga aattggaacc ttcaagcaat
     3301 agcgtggttg tggtcgagcc ccaccaggtg aaaaccgacc gctctaagca cgtgaaaatc
     3361 agttgcgatc agctgcgagc ccgaggagcc cggagagtaa ctgtcgaccc cctgggcaga
     3421 gtcagagtcc acgctcctgg agccagggtg ggtatcggcg gcgatgcagg acgcaccgca
     3481 atggagccag ccgaagatat ctca
//
