LOCUS       sequence-195187-        3156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E3CY56
FEATURES             Location/Qualifiers
     source          1..3156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1616..1624
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgattggag agcacgtccg cggcggctgc ctgtttgatg accattggac tcccaactgg
       61 ggtgctttcc gactgcccaa tactgtaaga acattcacca aagccgagaa cccaaaagac
      121 ggctcctccc tggccgaacc gcgacggcag gcacgcggcc tgagaagacg gttgaggcgc
      181 aagacccaga ggctcgaaga cctcagacgc cttcttgcta aggagggcgt gctgtcactt
      241 tcagaccttg agactttgtt ccgggaaacc cccgccaaag atccatatca gttgcgagct
      301 gagggtttgg acaggcccct gagcttccca gagtgggtca gagttctgta tcatattacc
      361 aaacaccggg gatttcagag caaccgaagg aatccagtgg aggacggcca ggaaagatcc
      421 agacaggaag aggaggggaa gctcctgtcc ggggtcgggg agaacgagcg gctgctgcgg
      481 gagggtgggt atcgcaccgc aggagagatg ctggcccggg atcctaagtt ccaggatcac
      541 agaaggaatc gcgccggtga ttactctcac accctgtctc gctccctgct gctggaggaa
      601 gcaagaagac tgttccagtc acagagaacg ctcggaaatc cccacgcatc atctaatctg
      661 gaggaagcat tcctgcatct ggtggcattt cagaatccat tcgcaagcgg cgaagacatc
      721 cggaataagg ccggtcactg ttccctggag ccagatcaaa tccgcgcccc tcgaagaagc
      781 gcttccgcag aaacctttat gctgctgcag aagaccggta acttgagact gattcatcgc
      841 agaacagggg aggaaaggcc ccttaccgat aaagagcggg agcagattca tttgctggca
      901 tggaaacagg agaaagttac tcacaaaact ctgaggcggc atctcgagat tcccgaggaa
      961 tggctgttta ccgggttgcc ctatcaccgg tcaggagaca aggccgagga gaaattgttt
     1021 gttcacctgg ctggaatcca cgagattcgc aaggctttgg acaagggacc tgaccccgca
     1081 gtttgggata cgcttcggag tcggagggac ctcctggata gcattgccga taccttgaca
     1141 ttttataaga atgaagacga gattctgcct cgactggaat cactgggact ctcacccgaa
     1201 aacgcacggg ccctggcccc cctgtctttt tctggaaccg cacacctgtc attgtctgcc
     1261 ctggggaagc tgctgcccca cctggaagaa ggaaagagct acacccaggc tcgagccgac
     1321 gcgggatatg cggccccacc tccggacaga caccccaagt tgcctcccct ggaagaagcg
     1381 gactggcgga accccgtggt gtttcgcgcc cttacccaga ccaggaaagt ggtcaatgca
     1441 cttgtgaggc ggtacggtcc cccttggtgc atacatctgg agactgcacg cgaattgtcc
     1501 cagcctgcaa aagttaggcg gcgcattgag actgagcagc aggctaacga gaagaagaag
     1561 cagcaggctg agagggagtt cctggacatt gtggggaccg cgccagggcc cggggacctc
     1621 cttaaaatgc gattgtggcg ggagcaaggt ggcttctgtc catactgtga ggaatatctg
     1681 aaccctaccc ggctggctga gcccggctac gcggagatgg accatatcct gccttattcc
     1741 aggagtttgg ataatggatg gcataacaga gtgctcgtgc atgggaagga caatcgcgat
     1801 aaaggcaatc gaaccccatt cgaagcgttc ggcggagata ctgcacggtg ggacagactg
     1861 gtggcctggg tccaggcaag tcacctgtca gccccaaaga agagaaacct gctgagggag
     1921 gactttgggg aggaagcgga aagggaactt aaagatagaa atttgaccga tactcgcttt
     1981 attacaaaga ccgcagccac ccttctgaga gacaggctga catttcaccc agaagctcca
     2041 aaagaccctg tcatgacact taacggaaga ctcactgcct ttctcagaaa gcaatggggg
     2101 ctgcacaaga accggaagaa cggcgacctg catcatgcac tggatgctgc ggttctggct
     2161 gtggcgtccc gaagctttgt gtatcgcctg agttcccaca acgccgcgtg gggcgagttg
     2221 cctagggggc gagaagctga aaatggcttc agcctcccat acccggcttt caggtctgag
     2281 gtgttggcga gactgtgccc gacccgggaa gagattctct tgcggctgga tcagggcgga
     2341 gtgggttacg acgaagcatt ccggaatgga ttgcggccag tgtttgtgtc tcgagctcct
     2401 tctagacggc tgcggggcaa ggctcacatg gaaaccttgc gaagccctaa gtggaaggat
     2461 caccctgaag ggccaagaac tgcgtctaga attcctctca aagacctgaa tcttgaaaaa
     2521 ctcgagagaa tggtgggcaa ggatcgagat agaaaactct atgaagccct tcgagagaga
     2581 ctggcggcct tcggaggcaa cggaaaaaaa gcttttgtag ctccttttcg caagccttgc
     2641 cggtccgggg agggccccct cgtgcgcagc ttgagaatat tcgactcagg atattccggt
     2701 gtggaactga gagatggcgg tgaagtatac gccgtcgctg atcacgaatc tatggtgcga
     2761 gtggatgtgt acgcaaaaaa aaataggttc tacctcgtac cagtgtacgt ggcggatgtg
     2821 gcccggggca ttgttaagaa cagagccatt gtggcccata aaagtgagga ggaatgggac
     2881 ctggtggacg gcagtttcga ctttagattc agtctttttc ccggggatct tgtagaaatc
     2941 gagaagaagg acggcgcata tctgggatat tataagtctt gccaccgggg agatggacgc
     3001 ctgctgttgg acagacacga tcgcatgccc cgagaaagcg actgtggcac cttttatgtc
     3061 tctacccgaa aggacgtctt gtctatgagc aagtatcagg tggaccccct cggcgaaata
     3121 agactggtgg gaagcgaaaa accacccttc gtgctg
//
