LOCUS       sequence-195189-        3543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E6KTN8
FEATURES             Location/Qualifiers
     source          1..3543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctgcact gcattgcagt cattcgggta cctcctagtg aagagcccgg cttcttcgaa
       61 acccatgcag atagttgcgc cttgtgtcac cacggatgca tgacctatgc ggccaatgac
      121 aaagctatca gatacagagt agggatagac gtcgggcttc ggtctatcgg gttctgcgca
      181 gtagaggtgg acgacgagga ccatcctatc agaatactga attcagtcgt ccacgttcac
      241 gacgccggga ctgggggccc aggcgaaact gaaagccttc ggaagcggtc tggcgttgct
      301 gcacgcgcca ggcggcgcgg aagagccgag aagcaacgcc tcaagaagtt ggacgtgctt
      361 ttggaagagc tgggatgggg tgtgtctagt aatgaactgt tggactcaca cgctccatgg
      421 cacatcagaa agcggttggt ttccgagtat atcgaggacg aaaccgaacg aaggcaatgt
      481 ctttccgttg ccatggccca catcgctagg caccgcggct ggcggaatag cttttctaag
      541 gttgacaccc tgctcctcga gcaggcccct agtgatcgga tgcaaggtct gaaagaaagg
      601 gttgaggaca ggaccgggct ccagtttagt gaagaggtga cacagggaga gctggtggct
      661 accctgctgg aacatgacgg agacgtcacg attcgcggat ttgtccgcaa gggaggtaaa
      721 gccaccaaag tgcacggtgt cctcgaagga aagtacatgc agagcgatct tgttgctgag
      781 ctcagacaga tctgccgcac ccagcgcgtc tccgagacta cttttgaaaa actggttctt
      841 tctattttcc attccaaaga gccagcgccc tctgccgcac gacagagaga gagggtagga
      901 cttgacgagc tgcaactcgc gctcgatcca gctgcaaaac aaccacgagc cgaaagggcc
      961 catccagcct ttcagaaatt caaggtagtg gccaccctgg caaacatgag gattagggag
     1021 cagagcgccg gggaacgaag cctgacttca gaagagctga atcgggtggc tcgctacctc
     1081 ctcaatcaca ccgaatcaga gagccccacg tgggatgatg ttgcccggaa actggaggtg
     1141 ccacggcacc gcctgcgagg ttcatccaga gccagcctgg agacaggtgg gggactgacc
     1201 tatccgcccg tggacgacac tactgtgagg gtgatgtccg cagaggtgga ctggctggcc
     1261 gactggtggg attgtgctaa tgatgaatca aggggccaca tgatcgacgc tatctctaac
     1321 gggtgcggat cagagcctga cgacgtcgaa gatgaagagg tgaacgagtt gatcagctca
     1381 gcaactgctg aggacatgct taaactggag ctgctcgcca aaaagctgcc gtctggtcgg
     1441 gtggcgtaca gtctgaagac cttgcgggag gtcaccgcag ccattctgga aacaggcgac
     1501 gatctttccc aagcaattac cagactgtat ggcgttgatc ccgggtgggt tcctacaccc
     1561 gccccaatcg aagcgccggt cgggaaccca tccgtcgaca gagttttgaa gcaggttgct
     1621 agatggctga agtttgcttc caagagatgg ggtgtccccc agacagttaa tatcgagcat
     1681 accagagaag ggctcaagtc agcgtccctc ttggaggagg agagggagag atgggagcgc
     1741 ttcgaggccc ggcgcgaaat cagacaaaag gaaatgtaca agagactggg cattagcggc
     1801 ccatttagac ggtccgacca agtgaggtat gagatactgg acctgcaaga ctgcgcatgt
     1861 ctgtattgcg gcaacgaaat caactttcaa acctttgagg tggatcatat aatcccgcga
     1921 gtggacgcct ctagcgattc ccgccgcacc aacctggcag cggtgtgtca cagttgtaac
     1981 agtgccaagg gtgggttggc atttgggcag tgggtgaaga ggggcgactg tccaagcggc
     2041 gtctcactgg aaaacgctat taaacgcgtc agatcttggt ccaaagacag actgggcctg
     2101 actgagaagg ctatgggcaa aaggaaatcc gaggtaatca gcaggctgaa gaccgaaatg
     2161 ccatatgaag agttcgacgg acgcagtatg gagagcgtcg cttggatggc tattgaactc
     2221 aagaaacgga tcgagggtta tttcaactcc gatcggcccg aaggctgtgc cgccgttcag
     2281 gtgaacgctt atagcggtag attgacagcc tgtgctcgga gggctgccca tgtggataag
     2341 cgcgtacgcc tgattcggct gaagggcgac gacggccatc ataaaaacag attcgaccgg
     2401 agaaaccacg caatggatgc actcgttatt gcgttgatga cgcccgccat cgcgcggacc
     2461 attgccgtgc gggaagaccg ccgggaggct cagcagctga cacgggcatt tgaatcctgg
     2521 aagaatttcc tgggctctga agaacgcatg caggataggt gggaatcctg gatcggagat
     2581 gttgagtacg cgtgtgaccg actgaatgag ctgatcgatg ccgataagat cccagtgaca
     2641 gagaacctga gacttaggaa cagcgggaaa ctccatgctg accagccaga aagcctgaag
     2701 aaggctcgca gaggaagtaa gcgacctcgc ccccagcggt atgtgctcgg cgacgccttg
     2761 cctgctgacg tgatcaatcg cgtgacagac cccggtctgt ggaccgcgtt ggtgcgggcc
     2821 ccagggttcg atagccagct ggggcttcct gcggatctga atagggggct gaagttgaga
     2881 ggtaagcgga tatctgctga tttcccaata gactacttcc caacagactc ccccgcattg
     2941 gcagtccagg gcgggtacgt aggcctggag ttccaccacg caaggctgta ccggatcata
     3001 ggccctaaag aaaaagtcaa gtacgctctc cttcgggtct gtgccatcga cctgtgtggc
     3061 atagactgcg acgacctctt cgaggttgag ctgaaaccaa gtagcatcag catgcgaact
     3121 gctgatgcca aactgaaaga ggctatgggt aacggctctg ccaagcagat tggatggctg
     3181 gtcctgggtg acgagataca gatcgatccc acaaaattcc ctaaacagag catcggcaaa
     3241 ttccttaagg agtgcggacc agtgtcctca tggcgcgtgt cagccctgga cacaccctct
     3301 aaaataactc tgaagcctag attgttgtct aacgagcccc tgctgaagac ctcccgcgtg
     3361 ggcgggcacg agtcagacct cgtagtggcc gaatgtgtgg agaagattat gaagaaaaca
     3421 gggtgggtcg tcgaaatcaa cgccctgtgc caatctggac tcatccgggt gatcaggcgg
     3481 aatgcactgg gggaagtgag gacatcccct aagtcaggcc tgcctatctc cctgaatctg
     3541 cgg
//
