LOCUS       sequence-195191-        3087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-F4GDP9
FEATURES             Location/Qualifiers
     source          1..3087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaggagct tgcggtatcg actggcactg gacctggggt caactagcct gggatgggcc
       61 ctctttcgcc tggatgcctg caacaggcca acggccgtta tcaaggccgg cgtgcggata
      121 ttttctgacg ggagaaaccc taaggatggc tccagtctgg ccgtgacccg acgggcggct
      181 agagccatgc gccgaaggcg cgataggctg ctgaaaagga agactcgaat gcaagccaag
      241 ttggtggaac atggattctt ccccgccgat gccggaaaac gaaaggctct ggagcagctt
      301 aacccttacg ccttgagagc taaggggttg caggaggccc tgctgccggg agaattcgcg
      361 cgggcactgt ttcatatcaa ccagaggaga ggatttaaga gcaataggaa gactgacaag
      421 aaggacaacg attcaggagt gttgaagaag gcaatcggcc aattgcgaca gcaaatggct
      481 gagcagggtt cacggaccgt tggcgaatac ctgtggacca gactgcagca gggccagggg
      541 gtcagggccc gatacagaga gaaaccatat acgaccgaag aaggaaagaa acgaattgat
      601 aaaagctacg acctgtacat cgacagagct atgatcgagc aggagtttga cgcactgtgg
      661 gctgcccagg ccgccttcaa tccgacactc ttccatgagg ccgcgagagc ggatctcaag
      721 gatactctgc tccatcagcg accactcaga cccgtaaagc caggcagatg tacactgctc
      781 cccgaagaag agcgggcacc tctcgctctt cctagcactc agcggttcag gatccaccag
      841 gaggttaacc acctgaggct cctcgatgag aacttgcgag aggtggcctt gactcttgca
      901 caacgggacg ccgttgtgac ggccctcgaa actaaagcta agctgtcctt cgagcagatt
      961 agaaagctgc tcaagttgag cggatctgtc caatttaatc tggaggatgc taagaggaca
     1021 gaactgaagg gcaacgccac aagtgccgcc ctggcgagaa aagaactgtt cggcgccgct
     1081 tggtcaggct ttgatgaagc tctccaagac gagatcgtct ggcagctggt gaccgaggaa
     1141 ggggaaggcg cacttatcgc atggctgcag acacataccg gcgtcgatga ggctcgggcc
     1201 caggctattg tggacgtatc cctgccagag ggttacggga atctgagtcg gaaggccctg
     1261 gccaggatcg tgcctgcctt gcgagccgcc gtaattacct acgataaggc cgtacaggca
     1321 gctgggttcg accatcactc acagctgggc ttcgaatacg atgcgtcaga ggtcgaggat
     1381 ctcgtgcatc cagaaaccgg agaaatccgc agcgtgttta agcagcttcc ttactatgga
     1441 aaagcactcc agcgccatgt ggcttttggg tctgggaaac ctgaagaccc cgacgagaaa
     1501 cggtatggga aaattgcgaa cccgacagtg catattggcc tgaaccaggt ccgcatggtg
     1561 gtgaacgcgc ttattaggcg ctacgggcgg cccactgaag tggttattga actggctcga
     1621 gatctgaaac agtcccgcga gcagaaagtg gaggcacaga gaagacaggc cgataaccaa
     1681 aggcggaacg ccaggattcg gagaagcatt gccgaggtcc ttggcatcgg agaggaacgc
     1741 gtgcgcggga gcgatattca gaaatggatt tgttgggagg aactgagctt tgacgcagcc
     1801 gaccgcagat gcccatattc tggagtgcag atctctgcag caatgctgct gtccgacgag
     1861 gtcgaagtcg agcacatctt gcctttttcc aagacattgg atgactcctt gaataacaga
     1921 acagtggcta tgaggcaggc aaacagaata aaaaggaacc gaactccttg ggatgccagg
     1981 gccgaatttg aggcccaggg ctggtcatac gaggacattc ttcaacgcgc tgaaagaatg
     2041 cccctcagga aacggtatcg ctttgcacct gacgggtatg agcgctggct cggcgatgac
     2101 aaggattttc tcgcgcgcgc tctgaatgat acgcggtacc tcagtagagt tgccgccgaa
     2161 tacctgcgac ttgtgtgtcc aggaacaagg gtaatcccgg ggcagttgac cgctctgctt
     2221 cgcggcaagt tcggactgaa tgacgttttg gggctggacg gtgagaagaa tcgaaacgat
     2281 caccgccacc acgctgtaga cgcttgtgtt attggcgtga ccgaccaggg actgatgcag
     2341 cgcttcgcta ctgcctctgc ccaagctagg ggggatggtc tgacccgcct ggtggacggt
     2401 atgcccatgc catggccaac atatagagat cacgttgagc gagccgtccg acacatatgg
     2461 gtgagccatc ggcctgatca cggtttcgaa ggagctatga tggaggagac aagttacgga
     2521 atacggaaag atggtagcat caaacagcga aggaaggccg atggatcagc gggccgcgag
     2581 attagcaatc tgattaggat tcacgaagca acacagcctc tgcgacacgg agtgagtgct
     2641 gatggccaac ctctcgccta caagggatat gtagggggta gtaattattg catcgaaata
     2701 actgttaacg acaagggtaa gtgggagggc gaagtgatca gtaccttcag ggcttatggc
     2761 gtcgtccgag caggtggcat gggtaggctt cggaatccgc acgagggcca gaacggcagg
     2821 aagctcatta tgcgacttgt tataggggac tcagtccgcc tcgaggtcga tggtgctgaa
     2881 cgcactatgc ggatcgtgaa gatttccgga agcaatggcc agatttttat ggctcccatc
     2941 cacgaagcta atgtagatgc acggaacaca gataaacagg atgctttcac ctacacatct
     3001 aaatacgccg gaagtcttca gaaggcaaaa acaaggcgcg tgacgatttc ccccatcggt
     3061 gaagttcggg atccgggatt taagggc
//
