LOCUS       sequence-195193-        3438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-J9E534
FEATURES             Location/Qualifiers
     source          1..3438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcataca ggctgggact cgatatcggc atcacaagtg tgggatgggc agtggtcgcg
       61 ttggagaagg acgagagcgg cctcaaacca gttaggattc aggacctcgg cgtacgcatt
      121 ttcgacaaag ccgaagatag caaaaccgga gcgagtctgg ctcttcctcg acgagaggca
      181 agaagcgcca gaaggcggac cagacgccgc aggcatcgac tgtggagggt taaacggctg
      241 ctggagcagc acggcatttt gtcaatggag cagattgaag ccctctatgc ccagcgcaca
      301 tcctcacccg acgtgtacgc cctcagagtg gcggggctcg acaggtgtct gattgccgag
      361 gagatcgctc gcgtcctgat ccatattgcg caccgacggg gattccagag caacaggaag
      421 agcgaaatca aggatagtga cgcgggcaag ctcttgaagg ctgttcagga gaacgagaat
      481 ctgatgcaga gtaaggggta caggaccgtc gctgaaatgt tggtgagtga ggccaccaag
      541 accgacgccg aaggaaaact cgttcacggg aagaagcacg gttacgtgtc caatgtgaga
      601 aacaaggccg gggagtatag acacacggtg tccaggcagg ccattgtgga cgaagtgcgc
      661 aaaatctttg ccgcacaacg ggcccttggg aatgatgtca tgtccgaaga actcgaggac
      721 agctacctga aaatcctgtg ttcacagcgg aacttcgatg atggaccggg aggtgacagt
      781 ccctatggcc acggaagtgt gtcacctgat ggcgtccgac agtctattta cgagcggatg
      841 gttggatcct gcaccttcga gactggcgag aaaagagccc cacggtcctc ctacagtttc
      901 gagaggttcc agcttctgac taaagtagtg aaccttagaa tctatcgcca acaagaggat
      961 gggggcaggt atccctgtga attgactcaa acagaaaggg cccgcgttat cgactgcgcc
     1021 tatgaacaaa ctaagattac gtatgggaaa ctgcgaaagc ttctggacat gaaggacact
     1081 gagagtttcg ccggcctcac ttatgggctg aaccggtcaa ggaacaaaac ggaggacact
     1141 gtctttgtag agatgaaatt ttaccacgaa gtgcgcaaag ccctgcagcg ggcaggcgtt
     1201 tttattcagg acttgagtat tgagacactg gaccaaatcg ggtggatact cagcgtttgg
     1261 aaatcagacg acaaccgccg gaaaaaactc tcaacactgg ggctgtctga caatgttatt
     1321 gaagagcttc tgccattgaa tggatccaag ttcggtcatc tgtccctcaa agccatcaga
     1381 aagatcctgc cctttctcga ggatggatac tcttacgatg ttgcctgtga gctggcgggt
     1441 taccaatttc aggggaagac cgaatatgtt aagcagcggt tgctgcctcc tttgggggag
     1501 ggagaagtga ccaacccagt agtaagaagg gcactgtctc aagccatcaa agttgtgaat
     1561 gctgtcatcc gcaaacacgg tagtcctgaa agcatccaca ttgaattggc tagagagctt
     1621 tccaagaatc tcgacgagcg ccggaagatt gagaaagccc agaaggaaaa ccaaaaaaac
     1681 aatgagcaga ttaaggacga gattagggag atcctcggat ctgcacacgt gacaggacgg
     1741 gatattgtta aatacaagct gttcaagcag cagcaagaat tctgtatgta tagtggcgag
     1801 aagctcgatg tcacacgctt gttcgaaccc ggttatgcag aggtggatca catcatcccc
     1861 tatggtattt ccttcgatga tagttatgat aataaggtcc tggtgaagac cgagcagaac
     1921 aggcagaaag gcaatcggac acccctcgag tatctgcgag ataagccgga gcagaaagcc
     1981 aagttcattg cattggtaga atcaatccct ttgtcccaga agaaaaagaa ccatctgctg
     2041 atggataagc gcgccatcga tttggagcaa gaagggttta gagagagaaa cctttcagac
     2101 acgcgatata ttactcgagc gttgatgaac cacattcagg catggcttct tttcgatgaa
     2161 actgcgtcta ccagatccaa gcgggtggtt tgcgttaacg gagccgtgac ggcctacatg
     2221 cgggcgcgct ggggattgac taaagaccga gatgccggcg ataagcatca cgcagccgac
     2281 gccgtcgttg tcgcttgtat aggagactcc ctgattcaga gagttaccaa gtacgataaa
     2341 ttcaagagga atgccctggc agataggaat cggtatgtgc agcaggtttc taaatccgag
     2401 ggcattacac agtacgttga taaagagaca ggtgaggtgt tcacatggga aagcttcgat
     2461 gagcggaagt tcctgccgaa cgagcccctg gaaccttggc cattcttccg cgacgaactg
     2521 cttgctcgac tgagcgatga cccgtctaaa aatattcggg cgatcggctt gctcacatac
     2581 tccgaaacag aacagataga tcctatcttt gtgtcccgaa tgcctactag aaaggttact
     2641 ggcgcagctc ataaggagac tatccgaagt ccgcgcattg ttaaggtgga cgataataag
     2701 ggcaccgaga ttcaggtggt ggtaagcaag gtcgctctca cagagctgaa gctgacaaaa
     2761 gatggggaga tcaaggacta ctttagaccc gaagacgacc cgcgcctgta caatacattg
     2821 agagagcgcc tggtacagtt cgggggagac gccaaggctg ctttcaagga accagtgtac
     2881 aagatcagca aggacgggtc cgtgcgaact ccagtgagga aagtgaaaat tcaagaaaaa
     2941 cttacactgg gcgtgcctgt gcatggtggt cgcggtatag ccgagaatgg gggcatggtc
     3001 cgcatcgatg tttttgcaaa gggcggcaaa tattactttg tgcccattta tgtggccgat
     3061 gttctgaaga gggagttgcc taatcggttg gcaactgcac ataagccata cagtgaatgg
     3121 cgggtcgtgg atgactcata ccaattcaag ttctccctgt atcccaacga tgccgtcatg
     3181 atcaagccca gcagagaggt agacatcact tacaaggacc gaaaggagcc tgtgggctgc
     3241 cggatcatgt acttcgtgtc cgccaacatc gcctcagcat ccatctcctt gaggacacac
     3301 gataattccg gggaattgga gggtttggga attcaaggac tggaggtgtt cgaaaagtac
     3361 gtggtcggac cgctgggcga tacccatccc gtctacaaag agcgaaggat gccgttccga
     3421 gttgagcgaa agatgaac
//
