LOCUS       sequence-195195-        3339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-K2FTY8
FEATURES             Location/Qualifiers
     source          1..3339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcggtacc gcgtaggcct ggatctggga accgcgtcag tgggagctgc cgtgttcagt
       61 atggatgagc agggtaaccc catggagctt atctggcact atgaacgcct tttctctgaa
      121 cccttggtcc ctgatatggg ccagcttaaa cccaagaaag ccgcccgccg attggcgagg
      181 cagcagagaa ggcagattga tcgaagggct agtcgactgc ggcgaatagc tatcgtttct
      241 cgcaggttgg ggatagcccc agggagaaat gactctggag tgcacggaaa cgacgtcccc
      301 actctccgcg ccatggctgt gaatgaacga atcgagctgg ggcaattgcg cgctgtgctt
      361 ctccgaatgg gcaaaaagcg ggggtacgga ggaacattta aagccgtacg caaagttgga
      421 gaggctggcg aggtggcctc aggagcctca cgcctggagg aggaaatggt ggcactggcg
      481 agtgtgcaga ataaggacag cgtaacagtt ggcgaatatt tggctgcacg ggtagagcat
      541 ggactgccta gtaaattgaa agtggccgcg aacaacgaat actacgcccc ggaatacgct
      601 cttttcaggc agtacttggg gctccctgcg attaagggcc gcccagactg tctgcccaat
      661 atgtacgccc tgcgccacca gattgagcac gagtttgaac gaatctgggc cacccagtct
      721 caatttcatg acgtgatgaa agaccatggc gtcaaggaag aaattaggaa cgcgatattt
      781 ttccagcgac ccctcaagtc tccagccgat aaggtgggac gatgttcact gcagacgaac
      841 ctgccacgcg ccccaagggc ccaaatcgca gcccagaact tccgcatcga gaagcagatg
      901 gccgatctgc ggtggggcat gggccgcaga gccgagatgc ttaacgacca ccaaaaggct
      961 gtgatcagag agctcttgaa ccagcagaaa gagctgagtt tccgaaaaat ctacaaggaa
     1021 ttggaaaggg ctggttgccc tggccccgaa gggaagggcc tgaacatgga cagggctgca
     1081 ctcgggggaa gagatgacct gtccggcaac acaactctcg ccgcatggag gaaacttggc
     1141 cttgaggatc gctggcagga actggatgaa gtgacacaga tccaggtcat taatttcctc
     1201 gccgaccttg gctcccccga gcagcttgat accgatgact ggagttgcag gtttatgggg
     1261 aagaatgggc ggcccaggaa tttttcagat gagtttgtgg catttatgaa tgagttgagg
     1321 atgacagacg gcttcgacag gctttctaag atgggattcg aagggggcag gtcttcctac
     1381 tcaatcaaag cccttaaagc tctcactgag tggatgatag ctcctcactg gcgggagaca
     1441 ccagagacgc accgggttga cgaagaggca gctatcaggg aatgctaccc agagagcttg
     1501 gcaaccccag cacagggggg ccgacagtct aagctcgagc ctccacccct gactggaaat
     1561 gaagtagtgg acgtcgctct gagacaagtt aggcatacca tcaatatgat gatcgacgac
     1621 ctgggttccg tgcctgccca gatcgtggtg gagatggcca gggagatgaa ggggggcgtc
     1681 acccggagga acgacatcga gaagcagaat aagcgatttg cttccgaacg aaaaaaagcc
     1741 gcccagtcca tcgaggagaa cggcaagact ccaacaccag cccgcatcct ccgataccag
     1801 ctgtggattg aacaaggtca ccagtgccca tattgcgagt caaatataag cctcgagcag
     1861 gcactgtccg gcgcttatac aaatttcgaa catatcctgc caaggacgct cacccaaatt
     1921 ggacgcaaaa gatccgagct tgttctcgct cacagagagt gtaatgacga aaagggcaac
     1981 cgaactccct atcaggcttt tggacacgac gataggaggt ggcgcatagt cgagcaacgg
     2041 gctaacgcat tgcccaagaa atcatctagg aagacccggc tgttgctcct gaaagacttt
     2101 gagggggagg ccctgaccga cgagtccatt gacgaatttg ccgacaggca gctgcatgaa
     2161 agttcatggc ttgctaaggt aacaacacag tggctcagtt ctctggggtc agacgtctat
     2221 gtctctaggg gtagtttgac ggctgaattg aggcggcgct ggggccttga caccgtcatc
     2281 ccccaggtac gattcgagag cggcatgcca gtggttgacg aggagggtgc agagatcacg
     2341 cctgaggaat ttgagaaatt caggctccag tgggaaggtc accgggtcac acgagagatg
     2401 agaacagacc ggcggccaga caagaggata gaccacaggc atcacctggt cgatgccatc
     2461 gtgactgccc ttacttctcg ctctttgtat cagcaatacg caaaggcatg gaaggtcgcg
     2521 gatgagaagc agagacacgg ccgggtggat gtgaaggtcg aactgcctat gcctatcctt
     2581 acaatccgcg atatcgcctt ggaggccgtg cggagtgtga gaatcagcca caagccggac
     2641 cgctaccctg acggccggtt tttcgaagct accgcttatg gcatcgctca acggctggac
     2701 gagaggtctg gtgagaaagt tgactggctc gtgtcacgca agtccctcac cgatctggct
     2761 cccgagaaga aatctataga tgtagataaa gtaagagcga atatctccag gatcgtcggc
     2821 gaggcgataa gactgcacat ctccaacatc ttcgagaaac gagtgagtaa aggcatgaca
     2881 ccacagcagg cactgagaga accaatcgaa tttcagggaa atatcctgcg gaaagtgcgc
     2941 tgcttttatt caaaagcgga cgattgtgtc aggatcgagc actcaagtag acggggacat
     3001 cactacaaaa tgctgctgaa cgacggtttc gcctacatgg aggtaccatg caaggagggg
     3061 attctctacg gagtgcctaa tctcgttcgc cccagcgagg ccgtaggcat caaaagggcc
     3121 cccgagtcag gtgattttat acgattctac aaaggagata ccgttaaaaa tatcaagact
     3181 ggaagagtct acacgatcaa gcagatcctg ggagacggag gaggtaagtt gatcctcacc
     3241 ccagtcacag agacaaagcc tgccgacctg cttagcgcta agtggggacg actcaaagtg
     3301 ggggggcgca atatccatct gctgcggctc tgtgctgag
//
