LOCUS       sequence-195197-        3246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A1IQ68
FEATURES             Location/Qualifiers
     source          1..3246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..3246
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVRV
                     FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIK
                     SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
                     DNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEF
                     GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
                     KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
                     NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
                     KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
                     EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
                     EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
                     RLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEF
                     KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
                     VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
                     DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKLRTLLAE
                     KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
                     LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
                     TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
                     LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
                     GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
ORIGIN
        1 atggctgctt tcaagccaaa ccccattaac tacattctgg gactggacat tggtattgca
       61 tcagtaggat gggctatggt cgagatcgat gaagatgaga atcctatatg tctgatcgac
      121 ctgggggtgc gagtgttcga gcgggcagag gtgcccaaga caggtgatag tctggcgatg
      181 gcacgcagac tggccaggtc cgtcagacgc ttgacccgcc gacgggccca taggctgttg
      241 agggccaggc gactgctgaa aagggaagga gtgcttcaag cagccgattt cgacgagaac
      301 gggcttataa agagccttcc aaatactcca tggcagttgc gcgccgcagc acttgaccgc
      361 aaactgacac cgctggagtg gagcgccgtt ctgttgcatc tcattaaaca cagaggttat
      421 ctctcccaga gaaaaaacga gggagagaca gctgacaagg aattgggtgc cctgcttaag
      481 ggagttgccg ataacgctca tgccctgcaa accggagact tcagaactcc cgccgagctg
      541 gctctgaaca agttcgagaa ggaatcaggg cacatacgca atcagagggg ggactacagt
      601 cacacattta gcagaaagga cctgcaggct gaacttatcc ttctgtttga gaaacagaag
      661 gagttcggta atcctcatgt gagcgggggc ctgaaggagg gcattgaaac attgctgatg
      721 acccagcgcc ccgctctgtc cggagacgct gtgcaaaaga tgctgggcca ctgtactttc
      781 gagccagccg aaccaaaagc ggccaagaac acttacactg ctgagcgctt catctggctg
      841 actaaactta ataacctcag gattcttgag caggggtccg agcgaccact gactgataca
      901 gaacgggcta cactgatgga tgagccatac cgcaaaagca aactgactta cgctcaggca
      961 cgcaaactgc tgggcctgga ggataccgcc ttcttcaagg ggcttaggta tggtaaggat
     1021 aatgccgagg caagcaccct catggaaatg aaggcatacc atgcgatatc ccgggcactc
     1081 gaaaaggagg ggctcaaaga caagaaatca ccgctcaacc tttccccaga acttcaagac
     1141 gaaatcggaa cagccttttc actgttcaag acagatgaag acattaccgg aagactcaaa
     1201 gatcgcatcc agcctgagat cctggaagca ctgctcaaac acatctcctt tgacaaattc
     1261 gtgcaaatat ctctgaaggc tctgcggcgg attgtgcctc tgatggagca gggcaaaagg
     1321 tatgacgaag cttgcgcgga gatttacggc gaccattacg ggaagaagaa cacggaggaa
     1381 aagatttacc tccctccgat cccggccgat gaaattagaa atccagtggt tctgagggca
     1441 ctcagtcaag cccgcaaggt gatcaatgga gtagtgaggc ggtatggcag tcctgcacgc
     1501 atacacattg aaaccgctag agaggtcggc aagtcattca aggacagaaa ggaaatcgag
     1561 aaacgacagg aggagaaccg gaaagacagg gaaaaagctg ccgcaaagtt cagagaatac
     1621 tttcccaatt ttgtgggaga acctaaatct aaagatattc tcaaactcag actctacgag
     1681 cagcagcatg gtaagtgcct ctattcaggg aaagaaatca atctgggacg gctcaacgaa
     1741 aagggttatg tagaaatcga tcacgctctc cccttcagta ggacatggga cgacagtttc
     1801 aacaataagg ttcttgtact gggctctgag aaccagaata aaggcaacca gactccatat
     1861 gagtatttca acggaaaaga caattcaaga gagtggcagg aatttaaggc gcgcgttgaa
     1921 acctcccggt ttccaaggag caaaaagcag cgaattctgc tgcagaagtt tgatgaagac
     1981 ggattcaagg aaaggaactt gaacgacacg aggtatgtaa atcgattcct gtgccagttt
     2041 gtagcggata gaatgcgact cactggcaaa gggaaaaaaa gggtgtttgc ctccaatgga
     2101 cagataacta acctgctccg agggttctgg ggtctgcgca aagtgcgcgc tgagaacgat
     2161 cgacaccacg cactggacgc cgtggtggtg gcctgtagta ccgtcgctat gcaacagaag
     2221 atcacccggt ttgtgagata caaagagatg aacgcattcg atggcaagac tattgacaag
     2281 gaaacgggtg aggtcctcca ccaaaaaacg catttccccc aaccttggga atttttcgct
     2341 caggaggtaa tgatccgcgt gttcggcaaa ccggatggaa aaccagaatt cgaagaggcg
     2401 gacacccccg aaaagctcag gacactcctc gccgagaaac tttcctcacg ccccgaggca
     2461 gtacatgagt acgtcacccc cctcttcgtg tccagagcac ccaacagaaa gatgtctgga
     2521 cagggacaca tggaaaccgt gaaatcagct aaacgccttg atgaaggtgt gagtgtgctt
     2581 cgcgtacccc tgactcagct taagctgaaa gatttggaga agatggtcaa cagggagcgc
     2641 gaacctaagc tgtacgaagc cctgaaggca agactcgaag cacacaagga cgatcccgcc
     2701 aaggcctttg ctgagccttt ctacaaatac gataaggcgg ggaatagaac tcaacaggtg
     2761 aaggcggtgc gggtggaaca ggtccagaaa accggtgtct gggtaagaaa ccataatggc
     2821 atcgctgaca acgcaactat ggtgcgggtc gacgtgttcg agaaaggcga taagtattat
     2881 ttggtgccta tctattcatg gcaggtggcc aaaggtatat tgccagaccg ggctgtcgta
     2941 cagggcaaag acgaggaaga ctggcagctg atagacgaca gcttcaattt caagttttct
     3001 ctccacccaa acgatcttgt ggaagtgatc actaaaaaag cccgaatgtt cgggtacttc
     3061 gctagttgtc accgcggcac agggaacata aatatccgca tccacgattt ggatcacaaa
     3121 atcggcaaga acgggatcct ggagggcatc ggcgtaaaaa cagcactgag ctttcagaag
     3181 taccagatag atgaactggg gaaggaaatt agaccctgcc gcttgaagaa acggcctccc
     3241 gttcgc
//
