LOCUS       sequence-195199-        3192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A5EIM8
FEATURES             Location/Qualifiers
     source          1..3192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1135..1146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaagcgga ccagtctgcg cgcgtacagg ctgggagttg atctcggggc taactcattg
       61 gggtggttcg tagtctggct ggacgatcat ggacagcctg agggcctggg ccccgggggc
      121 gtgaggatct tccctgatgg ccggaatcct cagagtaagc agagtaacgc cgccggacga
      181 cggctcgccc gcagcgcccg gaggcgccgg gaccgctacc tgcagcggag gggcaaactc
      241 atgggcctcc ttgtaaagca cggcctgatg cctgcagatg aaccagccag gaagagactg
      301 gagtgcctgg atccttacgg gctgcgagca aaggctctgg acgaagtgct ccctttgcat
      361 cacgttgggc gggccctgtt tcacctgaac cagcgccggg gacttttcgc caatagagcc
      421 atcgaacagg gcgacaagga cgcgtccgcc atcaaagcag cggcggggag actccagaca
      481 agcatgcaag cttgtggggc aagaaccctc ggagaattcc tgaaccgacg gcatcagctg
      541 agagccaccg ttcgggcccg gtcacctgtc ggcggcgacg tgcaggcccg gtatgaattt
      601 taccctacga gagcaatggt ggacgccgag ttcgaggcta tatgggccgc tcaggcccca
      661 catcacccaa caatgactgc ggaagcacac gacacaatca gggaagcaat ttttagccag
      721 cgggccatga agcgtccaag tataggaaaa tgcagcctgg atccagccac ctcacaggat
      781 gacgtggacg gcttccggtg tgcatggtcc caccctctcg cccagaggtt tcgcatctgg
      841 caagatgtta gaaatctggc agtggttgaa actggtccca cgagttccag gcttgggaaa
      901 gaggatcaag acaaggtggc tcgggctttg ctgcagaccg accaactttc cttcgacgaa
      961 ataagaggct tgctcggcct cccctctgac gcccggttta atctggaatc agatagaagg
     1021 gatcatctga agggcgatgc cacaggtgca atcctgagtg caagacggca cttcgggcca
     1081 gcatggcacg acaggagtct ggataggcag atcgacattg tggccctgct cgaatctgcc
     1141 ctcgatgaag ctgccatcat tgcctctttg ggtactacac acagcctgga tgaagccgcc
     1201 gctcaaaggg cattgtcagc tctgcttcct gatggttact gtcggctggg gctgagggcc
     1261 ataaagaggg ttttgcctct catggaagct ggccggacat acgccgaggc tgcgtccgcc
     1321 gcaggctacg accacgctct gctgcccggc ggcaaactga gtccgactgg ctatctgccc
     1381 tactacgggc agtggctgca gaatgacgtc gtgggaagcg acgatgagcg ggacaccaat
     1441 gagcgacgct ggggccggct ccccaaccct acagtgcaca ttggcattgg ccagcttcgg
     1501 cgcgtggtta atgagctgat acgatggcat ggcccccctg cagagatcac ggtggagttg
     1561 actagggatc ttaaacttag tccacggcgc ctggcagagc tggagagaga acaggcagaa
     1621 aatcagagga aaaatgataa gcgaacctca cttctccgaa aactcgggct gccagcttcc
     1681 acacataacc tcctgaaact gaggttgtgg gacgagcagg gtgatgtggc cagcgagtgc
     1741 ccgtataccg gggaggctat cgggctcgaa cggttggtgt ctgatgatgt agatattgat
     1801 catttgatcc ctttctccat ttcatgggac gatagtgccg cgaacaaggt ggtgtgcatg
     1861 agatatgcca atagagagaa gggaaatcgg accccttttg aggcctttgg tcataggcag
     1921 gggcgtccct acgactgggc cgatattgcc gaacgggctg ccagattgcc caggggaaag
     1981 cgatggaggt ttgggcctgg ggcccgagcg cagttcgagg aactgggaga ctttcaggcc
     2041 agactgctca acgagacttc atggctggcc agggtagcca agcagtactt ggccgcggtg
     2101 acgcatccgc atcgcattca cgtgctcccc ggacgcctca cagcactgct tcgggcaaca
     2161 tgggagctga atgatcttct gccagggagt gatgatcggg ctgccaagtc acgcaaggat
     2221 catcgacatc acgcaattga cgcattggtt gctgccctca ctgatcaggc tctgctgaga
     2281 cggatggcca acgcccacga cgatactaga cgcaagatcg aggtgcttct gccttggcca
     2341 actttccgga tagatctgga aacccgactt aaggccatgc tggtttccca caaaccagat
     2401 cacggcctgc aggcaagact ccacgaggat accgcttatg gcactgtgga gcacccggag
     2461 acagaagacg gagcaaacct cgtgtacagg aagacttttg tggacatttc agagaaagag
     2521 atcgatagga tccgggatcg gcgacttagg gacctggttc gcgctcatgt agctggagag
     2581 aggcagcagg gaaaaacttt gaaggcagcg gtattgagct tcgcacagag acgagatatc
     2641 gctggccatc ccaatggcat caggcacgtg cggctgacga agtccatcaa accagattac
     2701 ctggtaccta tcagagataa ggctggcaga atctacaagt cttataatgc cggagagaac
     2761 gctttcgtgg acatcttgca ggctgaatct ggcaggtgga tcgctcgagc cacaaccgtc
     2821 ttccaggcga accaggctaa cgaatctcac gacgcaccag ctgcgcagcc aattatgagg
     2881 gtcttcaagg gagacatgct gaggatcgac catgcaggtg ccgagaagtt cgtcaagatc
     2941 gtgcgcttgt ctccatcaaa caacctgctg tatctcgtgg agcaccacca ggccggggtg
     3001 ttccagactc gacacgatga ccctgaagac agtttccgct ggctgttcgc gtctttcgat
     3061 aaactgagag aatggaacgc tgaacttgtt cgcatcgata cgctcggaca gccgtggcga
     3121 agaaaaaggg gtttggaaac tggttcagag gacgccacgc gcatcggatg gacccgccca
     3181 aaaaaatggc ca
//
