LOCUS       sequence-195201-        3111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A7HP89
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaagaa tctttggttt tgacattggt accacttcaa tcggctttag cgttatagac
       61 tatagctcaa ctcagtctgc tggcaatatc cagcggttgg gggtcaggat attccctgag
      121 gctcgagatc ctgatgggac gccactcaat cagcagcgcc ggcagaagag gatgatgagg
      181 aggcagctgc gacgacggcg gatccgacgc aaggctctca atgagactct ccatgaggca
      241 ggctttctcc cagcctacgg tagcgctgat tggcctgtgg tcatggctga tgagccctac
      301 gagctgcgga gacgcgggtt ggaagagggc ctctctgctt acgaattcgg tagagccatt
      361 taccatctgg cccagcacag gcacttcaag ggccgagagt tggaggaaag tgatacccca
      421 gacccagacg tggacgacga gaaagaggct gcgaatgaaa gagcagcgac tctcaaggcc
      481 ctgaaaaatg agcaaacaac tctcggggct tggttggccc gccgcccgcc cagcgatcgg
      541 aagcgcggga tccatgcaca taggaacgtc gtggccgaag aatttgagag gctgtgggag
      601 gtccaatcaa agtttcatcc cgccctgaag tcagaggaga tgcgcgctcg aatcagcgac
      661 actattttcg ctcagcggcc agttttttgg agaaaaaaca cccttggcga atgtagattt
      721 atgcccggag aacccctgtg tcctaagggc tcatggctca gccagcagag gagaatgctc
      781 gagaaactga ataacctggc gatcgcaggc ggaaacgcta gacctctgga tgccgaagag
      841 cgagacgcta tcctgagcaa acttcaacaa caggcaagca tgtcttggcc cggggtgaga
      901 tctgctctga aggctctcta caaacaacga ggcgaaccag gcgcagagaa aagtctgaag
      961 ttcaatttgg aactgggggg ggagtccaag ctcctgggta acgcgctgga agctaaactg
     1021 gcagacatgt tcgggccgga ttggccggcc catccccgca agcaggagat acgccatgcc
     1081 gttcacgaga ggctttgggc cgctgactac ggcgagactc cagataagaa acgggttatt
     1141 attctgagtg agaaggaccg aaaagcgcat agagaggcgg ccgctaactc attcgtcgcc
     1201 gactttggaa tcaccgggga gcaggcagcg cagctccagg cgctgaagct gcccacaggt
     1261 tgggagccat acagcatccc tgccttgaat ctgtttctgg cggagctcga gaagggcgag
     1321 agatttggcg ccctggttaa tggacccgac tgggagggtt ggagaaggac caactttccc
     1381 caccggaatc agccaacagg ggaaatcctg gataaacttc ccagccccgc tagtaaggaa
     1441 gaaagagagc ggatcagtca gctgagaaat ccaacagtgg tccgaactca gaatgagctg
     1501 cgaaaggttg tgaataattt gataggcctg tatggaaagc ctgacaggat acgcattgag
     1561 gtcggccggg acgtgggaaa atctaaaaga gagcgcgagg aaatacagag tgggatccgg
     1621 cggaacgaga aacagcgcaa aaaggctacc gaggacctca taaagaatgg gattgcaaac
     1681 ccaagcagag atgacgtgga aaaatggatt ttgtggaagg aaggacaaga gagatgcccc
     1741 tatacgggcg accagatcgg ctttaatgcc ctttttcggg agggtcgata tgaggttgag
     1801 cacatttggc ctagaagccg gtcctttgat aacagcccaa gaaacaagac actgtgtaga
     1861 aaagacgtga acatagagaa gggcaatagg atgccttttg aagcctttgg acatgatgag
     1921 gaccgctggt ctgccattca gattagactg cagggcatgg tatctgctaa aggcggcacc
     1981 ggaatgtcac ctgggaaagt caagaggttc ctggcgaaga ctatgcccga ggattttgct
     2041 gcacgccagt tgaatgacac cagatatgcc gcaaaacaaa tccttgctca gttgaaacgc
     2101 ctgtggcctg atatgggtcc agaggcacct gtcaaggtcg aagccgtgac cggccaggtc
     2161 accgcacagc tgcgcaagct ctggactctc aacaacattc tcgctgatga tggcgagaag
     2221 acaagggctg accatcggca ccacgccatc gatgccctga ctgtggcctg tacacatcca
     2281 ggcatgacta ataaactttc ccgatattgg cagctccggg atgaccctcg agcagagaaa
     2341 cctgccctga cacccccttg ggatacgata agggctgacg ctgagaaggc tgtcagcgaa
     2401 atcgtggtat cccaccgggt gagaaagaaa gtcagcggac cacttcacaa ggagacgacc
     2461 tatggcgaca ccggaaccga cataaaaacc aagagcggca cctatcggca gtttgtcacc
     2521 cgaaagaaaa tagaatctct ttccaagggg gagctggacg agatcaggga cccgcggatc
     2581 aaggagatag ttgccgccca cgtggctggc aggggcggcg acccaaagaa ggcattccct
     2641 ccatatccct gcgtgtcccc cggggggcct gaaatccgga aagtccggct gacttccaag
     2701 cagcagctca acctcatggc ccagacaggt aacggatacg cagacctcgg cagcaaccac
     2761 cacatcgcta tatatcggct gcctgatgga aaagccgatt ttgaaattgt ctccctcttt
     2821 gacgcctctc gcagacttgc ccagcgcaac cctatagttc aaagaacacg cgctgacggc
     2881 gcgagctttg tgatgagtct ggctgctggc gaggcaatta tgatcccaga gggtagtaag
     2941 aaggggatct ggattgtgca gggcgtgtgg gcatctggtc aggtggtgct tgagcgcgac
     3001 accgacgctg accactccac cactacccgg ccgatgccca accctatctt gaaggacgac
     3061 gccaagaagg tgtcaatcga tcccatcgga agagtccggc cttctaatga t
//
