LOCUS       sequence-195203-        3237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A8LN05
FEATURES             Location/Qualifiers
     source          1..3237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgcctgg ggctggatat tggcacatca tctattgggt ggtggctcta tgaaaccgac
       61 ggggctggaa gtgacgctag aatcactggc gtcgtcgatg ggggagtgag aatctttagc
      121 gacggccggg accccaagtc cggtgccagc ctcgctgtcg accggagagc agccagagca
      181 atgcgccgca gacgagatcg gtacctccgc cgcagggcta ctctcatgaa agtgctcgca
      241 gagactggcc tcatgccagc ggaccccgcc gaagccaaag ccctggaggc attggatcca
      301 tttgccttgc gagcagcagg gctggatgaa cctctccctc ttccccacct cgggagagct
      361 cttttccacc tcaatcagag gcgaggtttt aagtctaatc gaaagacgga ccggggcgac
      421 aatgaatcag gcaagatcaa agacgccact gctaggctcg acatggaaat gatggctaac
      481 ggcgccagaa cgtatgggga atttctgcat aaaagaagac agaaagccac agatccgcga
      541 cacgtccctt ccgtccggac gcggttgagc atagccaacc ggggaggtcc tgacggtaaa
      601 gaagaggcgg ggtacgactt ttatcccgac cgccgccatc tcgaagagga attccataag
      661 ctttgggccg cacaaggagc acaccatccc gaactgacgg aaaccctgcg cgacctcctg
      721 tttgaaaaga tattctttca gcgccctctg aaggaacccg aagtgggcct gtgccttttc
      781 tccggacacc acggagtgcc gcctaaagat cccaggcttc caaaagccca tccactcact
      841 cagcgacgag tgctgtacga aactgtcaac cagttgagag taacagcaga tgggagggag
      901 gcaagaccac tgactagaga ggagcgcgac caagtcattc acgctctgga taataagaag
      961 cctacgaaaa gtctgtccag tatggtactt aaattgcctg cgcttgccaa agtgttgaaa
     1021 ctgcgggacg gtgaaaggtt tactcttgaa accggcgttc gggatgccat cgcctgcgat
     1081 cctctccgcg cgagccctgc gcatccagac cggttcggcc cgcgctggtc tattctcgat
     1141 gccgacgcac agtgggaagt gatttcccgc atcagaaggg tgcagtctga tgctgaacac
     1201 gcagccctgg ttgattggct gacagaggct cacggtcttg acagggcaca cgccgaagcc
     1261 acagcccacg cccctcttcc tgatgggtat ggacggctgg gcctgaccgc tactacccga
     1321 atactttacc aactcactgc cgatgtggtt acgtatgccg atgctgtaaa ggcctgcggg
     1381 tggcaccact ccgacggacg aacgggagaa tgcttcgatc gactgcctta ttacggagag
     1441 gtccttgaga gacatgtgat tcctggctca tatcatccag acgacgacga catcactcgg
     1501 ttcggaagga ttacgaatcc aactgtgcac attgggctga atcagctgcg cagactcgtc
     1561 aaccgcataa tcgaaaccca cggcaaacca caccagatcg tggtggaact cgcccgggac
     1621 ctcaaaaaat cagaagagca gaagcgggct gatatcaaaa gaatacggga taccacagaa
     1681 gcggccaaaa agcggtccga gaagctcgag gagctggaga ttgaggataa cgggcggaat
     1741 cggatgctct tgcgactttg ggaagacctt aatcccgacg acgccatgag gcgcttctgc
     1801 ccctataccg ggacgaggat ctccgccgcg atgatcttcg acggctcctg cgatgtcgac
     1861 cacatcctgc catatagtcg gacgctcgac gattctttcc ccaaccgaac tctgtgcctt
     1921 agggaagcaa ataggcagaa aagaaatcag acaccttggc aagcatgggg tgacacccct
     1981 cactggcatg caatcgcggc taacctgaaa aatctgcccg agaacaaacg ctggaggttc
     2041 gcgccggatg ctatgacccg gttcgaaggc gaaaatggat tcttggatag ggccctgaaa
     2101 gatacgcagt acttggcccg catcagccgc agctacctgg acactctttt tacaaagggg
     2161 ggccacgtgt gggtagtccc cggacggttc accgagatgt tgcgcaggca ttgggggctt
     2221 aactctctgc tctctgatgc gggccggggg gccgtcaagg ctaagaacag gaccgatcac
     2281 cgccatcacg ctatagacgc cgccgtgatt gccgccactg accccggact tcttaataga
     2341 atctctcggg ccgctgggca gggagaagcc gccggacagt cagcggagct gatagcccgg
     2401 gatactcctc cgccttggga aggctttcgg gacgaccttc gggtccggct ggatagaatt
     2461 attgtcagcc atagggcaga ccatgggcgg attgaccacg ccgcaagaaa gcaggggcgc
     2521 gacagcaccg ccgggcaact gcaccaggaa actgcctact ccatcgtgga tgatatccac
     2581 gttgccagtc gcaccgactt gctgagcctt aagccagctc agctgctgga tgaacccggc
     2641 aggtctgggc aggtgcgcga cccacagctt cgcaaagctc tgcgcgtggc caccggaggt
     2701 aaaaccggta aggactttga aaatgccctg cggtattttg cctctaagcc tggtccctat
     2761 caagccataa gacgggtgcg gattatcaag cctctccaag cacaagccag ggtgcccgtg
     2821 cccgcgcagg atccaattaa ggcctaccag ggaggaagca accacctctt cgagatctgg
     2881 cggcttcccg atggagagat tgaagctcaa gtgatcacct ctttcgaggc ccacaccctt
     2941 gagggcgaga agcggccaca tcctgccgcc aagagactgc ttagagttca caaaggggat
     3001 atggtggccc tggaacggga tggaaggcgc gtggtgggcc atgtccagaa aatggatatc
     3061 gccaatgggc ttttcatcgt gccccataat gaggcgaatg ccgatacaag gaataatgac
     3121 aaatccgatc ctttcaaatg gatacaaatc ggtgcaaggc ccgcaatcgc cagcggaatc
     3181 cgacgcgtgt cagtcgacga gattggcaga ctgagggacg gtggcacccg cccaatt
//
