LOCUS       sequence-195205-        3288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-A8REH9
FEATURES             Location/Qualifiers
     source          1..3288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1108..1119
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgatggagg tgtttatggg cagattggtc ctcggtcttg acattggtat tacaagtgta
       61 ggtttcggca tcattgacct ggacgaatct gagatagttg actacggagt gaggctgttc
      121 aaggaaggaa ctgctgccga aaatgaaacc cgacgcacca aaagaggagg caggcgactg
      181 aaaagacgcc gcgttaccag aagagaggac atgctccacc tgctgaaaca ggcaggaatt
      241 ataagtacaa gcttccatcc tttgaataac ccttatgatg ttcgggtgaa gggtttgaat
      301 gaaagactga acggagagga actggcaact gctctgctcc atctctgcaa gcacaggggc
      361 agctcagtcg aaaccattga agacgacgaa gctaaggcaa aggaagccgg ggaaactaag
      421 aaagtgttgt caatgaatga tcagctgctg aaaagcggaa agtatgtgtg cgaaatccag
      481 aaagaaagac tgcggacgaa tggtcatatc agagggcacg aaaacaattt caagactaga
      541 gcttatgttg acgaggcatt ccagattctt tcacaccagg atctgtcaaa tgaactgaag
      601 agcgctatca ttaccatcat ctcacgaaag aggatgtact atgatgggcc aggcggcccc
      661 ctgtctccca cgccctacgg caggtacaca tactttggcc agaaggaacc aatagatctc
      721 atcgaaaaga tgagaggtaa gtgctcactt ttccctaacg agccccgagc ccctaaactg
      781 gcttactctg ctgagctgtt taatctgttg aacgacctca acaatttgtc tatagaggga
      841 gagaaactta cgagcgaaca gaaagcaatg atcttgaaaa tcgtccacga gaagggcaag
      901 attacaccta aacagctggc caaggaggtg ggggtttcac tggagcagat aaggggcttc
      961 aggatcgaca ctaagggaag tcccctgctg tccgaactca cgggatataa gatgatccga
     1021 gaggtccttg aaaaaagcaa tgacgaacac ctggaggatc atgtgtttta tgacgagatc
     1081 gcggagatct tgacaaagac caaagacatt gagggcagaa aaaagcagat tagcgagctt
     1141 tctagcgacc tcaacgaaga aagtgtgcat cagctggccg ggcttacaaa atttaccgct
     1201 taccattcac ttagcttcaa ggcactgcgc ctgatcaacg aagaaatgct taaaacagaa
     1261 ctcaatcaga tgcaatctat tacgctcttt gggctgaagc agaataatga gctgtccgtg
     1321 aaaggcatga agaacattca agccgacgat acagcaatcc tttctccggt cgccaaacga
     1381 gctcagcgcg aaacctttaa ggtggtgaac cgcctgagag agatttatgg ggagttcgat
     1441 tctattgtag tggagatggc cagggagaaa aactctgaag agcagcgcaa ggcgatcagg
     1501 gaaagacaaa aattcttcga aatgcgcaac aaacaggttg cagatatcat cggagacgat
     1561 cgcaagatta acgcaaagct tcgcgaaaaa ctggtgctct accaggaaca ggacggcaaa
     1621 acggcgtact ccctcgagcc cattgatttg aaactgctca tcgacgatcc aaacgcctat
     1681 gaagtagatc atataattcc tatcagcatc tccctggacg attctataac taataaggtg
     1741 ttggttaccc atcgggagaa ccaggagaag ggaaacctca cgcccatctc tgcctttgtt
     1801 aaaggacgct tcactaaggg gtcccttgcg caatacaaag cctactgcct gaaactgaag
     1861 gagaaaaata taaaaactaa taagggctac agaaaaaaag ttgaacagta cctgctcaac
     1921 gaaaatgaca tctataagta tgatattcag aaagagttca tcaaccggaa tctcgtcgat
     1981 acaagctatg catctagggt ggttctgaac acactgacca cctacttcaa acagaatgaa
     2041 attcctacca aagtgtttac agtgaaagga tcactgacaa atgcttttcg ccgaaagatt
     2101 aatctgaaga aagatcgaga tgaggattat ggccatcatg ccattgacgc gctgattatt
     2161 gcatccatgc caaagatgag gctgctgagt acaattttct caaggtataa gatcgaggac
     2221 atctacgatg aatctacagg ggaggtgttt agctcaggcg atgacagcat gtattatgac
     2281 gaccgatatt tcgcttttat cgcttcactg aaggctatca aagtcaggaa attttcacat
     2341 aagatcgaca ccaagcctaa tcggagtgtt gctgacgaga ctatctacag taccagagtt
     2401 atcgacggca aggaaaaggt tgtgaaaaag tataaggaca tttacgaccc aaagtttact
     2461 gctctggcag aagatatctt gaataacgcc tatcaggaga agtatctcat ggctttgcac
     2521 gatccacaaa ccttcgatca aatagtgaag gtcgtgaatt attacttcga ggaaatgtcc
     2581 aaaagtgaaa agtatttcac gaaagataag aagggccgaa tcaaaatttc agggatgaac
     2641 cctctgagcc tttaccggga tgagcatggt atgttgaaaa agtacagcaa gaaaggcgac
     2701 ggccctgcga taacccagat gaagtatttt gacggggttc tcgggaacca catagatatt
     2761 agcgcgcact atcaggtgag ggataaaaaa gttgttcttc agcaaatctc tccatacagg
     2821 acggactttt actattccaa agaaaacggc tacaagttcg taactatcag gtataaggat
     2881 gtcaggtggt ccgagaagaa aaaaaaatac gttatcgacc aacaggatta tgccatgaaa
     2941 aaagccgaga aaaagattga tgacacctac gagtttcagt tcagcatgca tcgcgatgag
     3001 ctgattggca tcaccaaggc cgaaggcgaa gccctcatct accccgatga aacgtggcat
     3061 aatttcaatt tcttctttca tgccggcgag actcccgaaa ttctcaagtt tacggccacc
     3121 aacaacgaca agtctaacaa gatagaggtc aagcctatcc actgctattg taaaatgagg
     3181 ctgatgccaa cgatctctaa aaagatagtg agaattgaca agtatgcaac tgacgtcgtc
     3241 gggaatctct ataaagtcaa gaagaatacc cttaagttcg agtttgat
//
