LOCUS       sequence-195207-        3195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B1UZL4
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             285..293
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             1121..1129
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgaacaaag acatcaacta cgccctgggg ctggatatcg gcatcacctc cgtcggctgg
       61 gcagttataa acctggattt gaaccgaatc gaagatctgg gcgtgagaat ttttaacgca
      121 gccgagaatc tcaaagatgg ttcatcattg gctctcccga ggcggcttgc acgcggacga
      181 agaaggctcc tgcgcaggaa agcataccga gtcgaaagag tgcggaagct gattcttaaa
      241 aacaacatac tcaccaagga ggaattggag ggtttgttca aaaataagga ggtgatcgac
      301 gtctgggaag cccgcgtgaa aggtctggat tacaaactgt ctagagaaga gtgggccaag
      361 attctcatca atttttgcaa gaggcgcggt tttaagagca acagaaaaaa tgaagccaag
      421 gataaggaag caggacaaat attgtccagc atatcttaca acatcgaaaa gatgaaggaa
      481 accaacagta ggacgatcgg cgagtatatc tataacgaag tgaagaattc cgaggattca
      541 tacgccccac ttcgcaataa gttcggcgaa tataaaatgt gtgtgagccg agatgtgatc
      601 cgggaagaga ttcataccct ttttgagaaa cagcgcgagc tgggcaataa ttttgccagc
      661 gatgaaatcg aggataagta tttcgagatt ttcaattctc agcggccttt tagcaacttc
      721 gaggacctgg aaaagctcgt gggtttttgc actttcgagc gaaagaaaca taaacgagca
      781 ccaaaacact gcatcagcag cgaagagttc acactttatg aaggcatcaa caagctgtca
      841 attattaagg acggtgagaa gagaaagctc tcagaggagg aacgaactct tgtcgttaac
      901 gaggcattca ataaaaagga aattaaatat actactctgc ggaagctcct tggcctctcc
      961 gaggaggagc tgttttctac actgacatat tccatcgaca aagacatttc caaaactgaa
     1021 aacaccaagt ttgtctcatt gaaaggatac catgagatta aaagggccat tgaaaagggc
     1081 gtgagtaaaa aggcgtggca ggatatcgag aataatagaa acctccttaa tgatatcgcc
     1141 tacgtgttga cactggggaa aacagatgat gagattgaga agcagttgag gctgaggaac
     1201 gtcccagagg agcttttcga atcacttctt gatatgtctt ttaataagtt taacaatctt
     1261 tctatctacg ccttggagaa aatactccct tttatgaaag aggggtatca gtacaatgaa
     1321 gcatgcgaaa aggccggcta caacttcaag gcgatctatg agggcgtcag aaccaagaag
     1381 ctccccgtaa tcgaaataga cgaaatagtt aatcccgtgg ttaacagggc tctggctcag
     1441 acaagaaaag tgatcaactc agtgatcgac aagtacggat ctcctgtgcg gattaacatc
     1501 gagctggcca gggatctggc aaagaatttc aaggacagaa aactcatcga gaaagaacag
     1561 aaagaaaata gggcaaacct ggacaagatt cgcgagaacc tccgggaatt gatggacaag
     1621 gagccaacag tcgtcgaggt gctgaagtat aggctctggg agcagcaaag gggggaatgc
     1681 gcctacaccc aatctcaaat acctctggaa agactcttta gtccaggata ctgcgagatc
     1741 gatcatatta ttccctttag ccgctccttc gatgattcac tgagcaacaa aatcctggtg
     1801 ttgggctcag aaaaccagag gaaaggtaac cgcactcctt acgaatattt tggtgaagac
     1861 tctgagagat ggaattcctt cgagatctgg gtgaaagggt cctatctgaa ctataagaaa
     1921 aaggccaatc ttttgaaaaa aaaattttcc gaagaagaac agcgcgactg gaaagcccgc
     1981 aaccttcagg acactaagta catctgcaga tacatcagca actttgtgaa taaccggctc
     2041 gaattcaaag aaagcgagaa taaacagaag gtgatcaccg tgaatggtcg agccactgct
     2101 tatctgagga gtcgctgggg cctcaacaag gtgagagagg atggcgataa gcatcacgcc
     2161 ctcgatgctg ccgtggtcgg ggtgacaacg caggggatgg tgcagaaaat cagcaagtat
     2221 agtaaagcac acgaattgta tacagtgaga aaaaatgatg aattcgtgga tgtcgagaca
     2281 ggcgagtgtg tagatgtcga cgaatataaa gaaataagaa aggacattct gcctcgacca
     2341 tggagaggat ttagtgagga actgaaactt aggctcagtg acaaccccct ggaggagctg
     2401 aaaaagagcc ccatagagac gtacaatgag gagttcatta aggagaacgt gaaacccatt
     2461 tttgtatccc gcgtaccatt ccgaaaaatc tcaggtaaac tgtttaaaga aaccatttat
     2521 tccgaaagag ctttcaaaga tggatacttc gtgagcaaaa aatctctcgt cgacctcaag
     2581 cggggcgacc tggacaattt ctacaattat gaatgtgaca aaaaacttta cgatgctatt
     2641 tcagaacgga tgaaagagtt caactacaat ggcaagaagg cctttgagag tccattccgg
     2701 aagcccacca agtctggcaa ggagggacct gtcgtgagat ccgtgaaaat taaaagtgcc
     2761 gtgccgttta aagacggcat tcccatgaat gagggtctgg tcgctaaaga ggggatggtg
     2821 agaatagata tttacgagaa ggagggcaag tacttcgtgg tgcccgttta ccggtaccag
     2881 ctggccaagg gaatcgtccc taaacgcgcg gcgatagcaa agaaagatga gctgaagtgg
     2941 cctatcatgg accagactta taacttcaag ttctcaattt acaaaaacga ccttattgag
     3001 gtgaagtatg ataagaaagg tgtctatttc ggctactatg acgggttcga ccgcaactca
     3061 gcatccttga caattgaaaa gcacgataat tctgagcgct accgaagcat cgggctgaag
     3121 gttggcgttc tggaaataaa taaatatgag gtcgatgtac tcggcaacta ccataaagta
     3181 aagcttggcg gtaat
//
