LOCUS       sequence-195209-        3585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B2KB46
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcaaaaga atatcaacac aaaacagaac cacatttata tcaaacaggc ccagaaaatc
       61 aaagaaaagc tcggagataa gccctacagg attggcttgg acctgggtgt gggcagtatt
      121 gggtttgcca tagtttccat ggaagaaaac gacggtaacg tgctgcttcc taaggagatt
      181 atcatggtgg gaagccgcat ctttaaggcc tctgccgggg ccgccgaccg gaaactcagc
      241 aggggccaga gaaacaacca tcgccacacc cgggagagga tgaggtatct ttggaaggtc
      301 ttggctgaac agaagcttgc cctgccagtc ccggccgacc tcgatcgcaa agagaacagc
      361 agtgaaggtg agacaagcgc taaacgattc ttgggagacg tcctgcaaaa agatatttat
      421 gaactgcgcg tcaagtctct ggacgagagg ttgagcctcc aggagctggg atacgtgctg
      481 tatcacatcg ccggacaccg cggaagttca gccatccgca cctttgagaa cgatagtgag
      541 gaggcccaga aggaaaatac tgagaacaag aaaatcgccg gcaatatcaa gcgactgatg
      601 gcgaagaaaa actatcggac atacggcgag tatctgtata aggagttttt cgaaaataaa
      661 gaaaagcaca aacgggaaaa aatctcaaac gcagcgaata accacaagtt tagtcctacc
      721 cgagatctgg tgattaagga agctgaagcg attctgaaga aacaggcggg gaaagacggc
      781 tttcacaagg agctcaccga ggaatatatc gagaagctca ctaaagctat cggatatgag
      841 agtgaaaaac tcatacctga atccgggttc tgcccatacc tgaaggacga gaaacgactg
      901 cctgcaagcc ataagcttaa cgaagagaga cgcctttggg agacacttaa caacgcaagg
      961 tactctgatc ccatcgtgga tatcgtcact ggtgaaatca caggatatta cgagaagcag
     1021 tttaccaagg agcagaagca aaagcttttc gactacttgc tgaccggatc agaactgacc
     1081 cctgcccaga ctaagaaact gctggggctc aagaatacta actttgaaga cattatactc
     1141 caaggaagag ataagaaagc ccagaaaatc aagggctaca agcttatcaa gctcgagagt
     1201 atgccttttt gggcccgcct cagtgaggca cagcaggact cattccttta tgactggaat
     1261 tcctgcccag atgagaaact gctgacagag aagttgtcaa atgaatacca cctgacggag
     1321 gaggagattg acaacgcgtt caatgagata gttctgagct ccagctacgc ccctctgggc
     1381 aaaagtgcga tgctgatcat cctcgagaaa atcaaaaatg atctgtcata cacggaagca
     1441 gttgaggagg cactgaagga aggaaaactg acgaaggaaa aacaggccat taaggatcgg
     1501 ctcccatatt atggagccgt gcttcaggaa tcaacccaga aaattatagc aaagggtttc
     1561 tccccgcagt ttaaagataa ggggtacaag actcctcaca cgaacaagta cgagctggaa
     1621 tacggtagaa ttgcgaaccc agtcgtgcat cagactctta acgaactgcg aaaacttgta
     1681 aatgagatca tcgacatcct gggcaagaag ccctgcgaga tcgggctgga aaccgccagg
     1741 gaattgaaga agagtgcaga ggatcggtct aaactgtctc gcgagcagaa tgataacgag
     1801 tccaatagaa atcgaatcta tgaaatctac atccgacctc agcagcaggt tatcattacc
     1861 aggcgggaga accctcggaa ctatattctt aaattcgaac tgctcgagga gcaaaaatcc
     1921 cagtgccctt tctgcggcgg tcagatcagc ccaaatgaca ttattaataa ccaggccgat
     1981 atagagcacc tttttcccat agcagaaagt gaagacaacg gcaggaataa tctggtcatc
     2041 agccacagcg cctgtaacgc cgacaaggcc aagagatctc cgtgggcagc ttttgcatcc
     2101 gccgctaagg atagcaaata tgattataac agaattctct ccaacgtcaa ggagaacatc
     2161 cctcacaaag cgtggagatt caaccaggga gcatttgaga aattcataga aaataaacct
     2221 atggccgcca ggtttaaaac cgataacagc tacatctcaa aggtggccca caagtacttg
     2281 gcttgcctgt tcgaaaaacc aaatattatt tgtgttaagg gatccctgac tgcccaattg
     2341 aggatggctt ggggcctgca gggccttatg atccctttcg cgaagcagct catcacagag
     2401 aaagagtccg aaagtttcaa taaagatgtc aactccaaca agaagattag actcgacaat
     2461 agacaccacg ccttggacgc tattgtgatt gcctacgcgt cccggggcta tggcaatttg
     2521 ctgaacaaaa tggcaggcaa agactataaa attaactact cagagcgcaa ttggctgtca
     2581 aaaattctgc tgcctccgaa taatatcgtc tgggagaata ttgacgctga tctcgagagt
     2641 ttcgagagct ccgtgaaaac tgcactgaaa aacgccttca ttagcgttaa acacgatcat
     2701 agtgacaacg gagagcttgt caagggtaca atgtacaaaa tcttctatag cgagcgcggc
     2761 tacaccctga ctacgtacaa aaaacttagt gccttgaagc tcaccgatcc tcagaaaaaa
     2821 aagaccccca aggactttct tgagacagcg cttctgaaat ttaagggccg ggagtccgag
     2881 atgaagaacg agaaaatcaa gagcgccatt gaaaataaca agaggctgtt cgacgtcata
     2941 caagataatc tggagaaagc aaagaagctt ttggaggagg agaacgagaa gagtaaggcg
     3001 gagggtaaaa aggagaagaa tatcaacgac gcgtccattt accagaaggc gattagcctg
     3061 tctggcgaca agtatgttca actctccaag aaggaacctg ggaagttttt tgcgattagt
     3121 aagcctactc ccactaccac aggatacggg tatgatacag gggattcact ttgcgttgat
     3181 ctctattacg ataacaaggg caaactgtgc ggagagatca tacggaaaat agatgctcag
     3241 cagaaaaacc ctcttaaata caaagaacag ggcttcacct tgttcgaaag gatttacggc
     3301 ggcgacattt tggaagtgga tttcgatatt catagcgaca aaaacagctt tagaaacaac
     3361 actggcagcg ctccagagaa ccgcgtcttt atcaaagtcg gtacattcac tgaaatcact
     3421 aacaataata tccagatctg gtttgggaat atcataaaaa gtacaggcgg gcaggacgat
     3481 tcttttacca ttaattccat gcagcagtac aatcccagga aactgatcct gagttcctgt
     3541 ggtttcatta aatacagatc tccaattctg aagaacaagg agggc
//
