LOCUS       sequence-195213-        4104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B5XLC1
FEATURES             Location/Qualifiers
     source          1..4104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4017..4025
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggataaga aatattctat aggacttgac atagggacaa atagtgtggg ctgggccgtg
       61 atcaccgacg attataaagt gccgtcaaag aagttcaaag tgctgggcaa taccgaccgg
      121 cacagtataa agaaaaacct gatcggggcc ctgctgtttg actccgggga aacagccgag
      181 gccactcgcc ttaaaagaac agctaggcgg cgatacacca ggcgcaaaaa tagaatctgc
      241 tatctgcagg agattttttc aaatgagatc gccaaggtgg atgatagctt ctttcaccgg
      301 ttggaggagt ccttcttggt cgaggaggat aaaaaacacg agcgacaccc tatctttggc
      361 aatatcgtag acgaagtcgc atatcacgag aaatatccta caatctacca cctgcggaaa
      421 aagttggccg acagcaccga caaagccgac ctgaggctga tctatctggc ccttgcccat
      481 atgataaaat tccgaggaca tttcctgatc gaaggagatc tcaaccccga taatagcgat
      541 gtggataagc tgttcattca gctcgtgcag acctacaatc agcttttcga ggaaaatccc
      601 atcaacgcct ccagggtcga cgccaaggct atcctgagcg ctaggctttc taagagtagg
      661 cgactggaga accttatcgc tcagctgccc ggagagaagc ggaacggtct gttcggcaac
      721 ctgattgccc tgtcactcgg cctgacaccc aattttaagt ccaacttcga cctggctgag
      781 gatgccaaac tccagctgag caaggacact tatgatgatg atctggacaa cctgctggct
      841 cagatcggtg accagtacgc agacctcttt ctggccgcca aaaacctcag cgacgctata
      901 ctcctgtcag acattcttag ggtgaataca gaaataacca aagccccttt gtccgcatcc
      961 atgatcaaaa gatatgatga gcaccaccag gatctgactc tccttaaagc gctggtccgg
     1021 caacagctgc cagaaaagta taaggaaatc ttctttgatc agagcaaaaa cgggtatgcg
     1081 ggttacatcg acgggggggc ctcacaggaa gagttttaca aattcattaa gcctatcctc
     1141 gagaagatgg atggcactga agagctcctg gcgaaactga atcgcgaaga ccttctgcgg
     1201 aaacaacgga catttgataa tgggagcatc cctcaccaga ttcacctggg ggaactgcat
     1261 gccatactta ggcggcagga ggacttctac cccttcctga aggacaaccg ggaaaaaatc
     1321 gaaaaaattc tgacgttcag aataccgtat tacgtgggac cgctggccag gggaaacagc
     1381 aggtttgcct ggatgactcg gaagtccgag gaaacgatca ccccctggaa tttcgaggag
     1441 gtcgttgaca agggagcatc cgcgcagtct tttatcgaaa gaatgacgaa ttttgataaa
     1501 aacctgccta atgaaaaagt tctccctaaa cattctctgc tctatgaata cttcaccgtg
     1561 tataatgagc tcaccaaggt taaatatgtt actgagggca tgcgcaaacc cgcgttcttg
     1621 agcggcgagc agaaaaaggc cattgtcgac cttctgttta agactaacag gaaagtcaca
     1681 gtcaagcagc tcaaagaaga ctatttcaag aagattgagt gtttcgatag tgtggagatc
     1741 tcaggcgtcg aggacagatt taacgcctca ctgggaacct accacgatct cttgaagatc
     1801 atcaaagaca aggatttcct ggacaatgag gagaacgaag atatcctcga ggatattgtg
     1861 ttgactctca ccctttttga ggatcgcgaa atgattgaag agaggcttaa gacttatgcc
     1921 cacctctttg atgataaagt gatgaaacag ctgaagaggc ggagatacac tggttggggt
     1981 cggctctccc gcaaactcat caacggaatc agggacaagc aaagtggtaa aaccatattg
     2041 gattttctta agagtgacgg ctttgctaac cgcaacttca tgcagctgat tcatgacgac
     2101 agtttgacat ttaaggagga cttgcagaaa gctcaagtga gcgggcaggg ggacagtctg
     2161 catgaacata ttgctaattt ggctggctct ccagcgatta agaagggcat tctccagaca
     2221 gtgaaggtgg ttgacgaact ggtcaaggtc atgggtcggc acaagcctga gaacatcgtt
     2281 atagaaatgg ctcgcgaaaa ccaaacaacc cagaaaggtc agaagaatag tagggagcgc
     2341 atgaagcgca tcgaagaggg catcaaggaa ctcgggagcg atattctcaa agagtacccc
     2401 gtagaaacga cacagctgca gaatgaaaaa ctgtatctgt attacctcca gaacgggagg
     2461 gacatgtatg tcgaccaaga gctggacatt aaccgactga gcgactacga tgtcgaccat
     2521 attgtacccc agtcattcct caaagacgac agtatcgata acaaagtgct gacaagatcc
     2581 gacaaaaata ggggaaaaag tgacaatgtc ccttccgaag aggttgtcaa aaagatgaaa
     2641 aattactgga agcagcttct caacgcaaag ctgattacac agcgcaagtt cgataacctg
     2701 accaaagctg agcgaggtgg tttgtcagaa cttgacaagg ctggctttat caagagacag
     2761 ctcgtcgaaa ccagacaaat caccaagcac gtagctcaga tcctggactc ccggatgaat
     2821 acaaagtacg acgagaacga caaactgatt agagaggtgc gagtgataac tctcaaatcc
     2881 aaacttgtga gcgatttcag gaaggatttc cagttctaca aagtgcgaga gataaataat
     2941 tatcaccacg cgcacgatgc ttacctcaat gctgtggtgg gcacggccct tattaagaag
     3001 tatcccaagc tggaatccga attcgtttat ggtgattata aggtgtacga cattagaaag
     3061 atgattgcaa agagcgagca agaaatcggg aaggccacag ctaagtattt cttctattct
     3121 aacatcatga attttttcaa aacagaaatc acactcgcta atggtgagat tcggaaaaga
     3181 ccacttatcg aaaccaatgg tgagacagga gagatcgtgt gggataaggg acgagacttc
     3241 gcaaccgtca ggaaggtact ctccatgccc caggtgaata tcgtcaagaa aactgaggtg
     3301 cagacaggtg ggttctcaaa agaaagtatc ctgccaaaac ggaatagcga caaactcatt
     3361 gcccgcaaga aagactggga tccgaagaag tacggaggct tcgattcccc aacagtggcc
     3421 tacagtgtcc tggttgtcgc aaaagttgaa aagggtaaaa gtaagaaact gaagtccgtc
     3481 aaagaacttg tcggtatcac gattatggaa cgcagctcat tcgaaaagga cccagtggat
     3541 ttcctggagg ctaaggggta caaagaggta cggaaagacc tgattattaa gttgccaaaa
     3601 tacagcctgt tcgagctgga aaacgggagg aagcggatgc tcgcctctgc cggggagctg
     3661 cagaagggaa acgagctggc cttgcccagc aagtatgtga acttcctgta cctggcttca
     3721 cactacgaaa agcttaaggg atcacccgag gataacgaac agaagcagct gtttgtcgag
     3781 cagcacaaac actatctgga tgagatcata gaacagatca gcgagttttc caagcgagtg
     3841 atactcgccg atgcaaacct ggacaaagtg ctcagcgcgt ataataaaca ccgggacaag
     3901 ccaatcagag agcaagccga gaacattatt cacttgttta cactgaccaa cttgggtgct
     3961 cctgccgctt tcaaatgctt cgatactacc atcgggagga acagatataa atcaattaag
     4021 gaggtgttgg atgccaccct cattcaccag agtatcaccg gattgtacga aacccggatt
     4081 gacttgagcc aactgggggg cgat
//
