LOCUS       sequence-195215-        3063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-B8I085
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1204..1215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaagtata ccctcggact tgacgtggga atcgcttctg ttggatgggc agtaattgac
       61 aaggataaca ataagatcat cgacttgggc gtaaggtgct tcgataaagc agaggaatct
      121 aagaccgggg agtctctcgc taccgctcgc agaatagccc gcggcatgag aagacggatt
      181 tcaagacgct cccaacggct tcggcttgtg aaaaaactct ttgtgcagta cgagattatt
      241 aaagatagca gtgagtttaa cagaatattc gacactagca gagatggatg gaaagaccca
      301 tgggagctga gatataacgc cctcagccgc atactcaagc cctatgagct tgtccaggtt
      361 cttacccaca ttacgaaaag acgaggattc aagtccaaca gaaaggagga tctgtcaacg
      421 acgaaggagg gagtcgtaat aacttctata aagaacaact cagaaatgct gagaactaaa
      481 aactatcgaa caataggaga gatgatcttc atggaaaccc ctgaaaattc aaacaagaga
      541 aataaggtgg atgagtacat tcataccatt gcgagggaag acctgctgaa cgaaatcaaa
      601 tacattttct ctatacagag aaaattgggg tcccccttcg tgaccgagaa gttggagcat
      661 gattttctga acatctggga attccaacgc ccgttcgcca gtggcgacag catactgtct
      721 aaggtgggca aatgcacttt gctcaaggag gagctgagag cacccacctc ctgttatact
      781 agtgaatatt tcgggttgct gcagagcatt aataatctcg tcctcgttga ggataataac
      841 acactgactc tgaacaacga ccagcgcgcc aagatcatcg agtacgcgca ctttaagaat
      901 gaaataaaat actctgagat ccgcaaactt ttggatattg agccagagat actctttaaa
      961 gctcataacc tgacacacaa gaatccttcc ggaaataacg agagcaagaa gttctatgag
     1021 atgaagtcct atcacaaact gaaatctact ctgccaactg atatatgggg aaagttgcac
     1081 agcaacaagg aatctcttga caacctcttc tactgtctga cggtctataa aaatgacaac
     1141 gagataaagg attacttgca agcaaataac cttgattacc tgatcgagta cattgcaaaa
     1201 ctccctacct tcaataaatt taagcacctt tccctggtag cgatgaagag gataattccc
     1261 ttcatggaaa agggttataa gtactctgat gcctgtaata tggcagagct cgatttcaca
     1321 ggatcatcta agctcgagaa gtgcaacaaa ctgactgtcg agccaattat cgaaaatgtg
     1381 actaaccctg tcgtgatccg agccctgact caagctagaa aagttattaa tgctattata
     1441 cagaagtatg gtctgccata tatggtcaac attgaactgg cccgcgaggc tggcatgacg
     1501 cggcaagaca gagataactt gaagaaagag cacgaaaata accggaaggc acgcgagaag
     1561 atcagcgacc ttattaggca aaatggtaga gtagcctcag ggttggacat cctgaaatgg
     1621 cggctgtggg aggaccaggg gggacggtgt gcctactccg ggaagcctat tcctgtgtgt
     1681 gatctcctga atgattccct gactcaaatt gaccacattt acccctactc acggagcatg
     1741 gatgactcat atatgaacaa agtgttggtt ctcaccgacg aaaatcagaa caagcgcagt
     1801 tacacaccat atgaggtgtg ggggtctaca gaaaagtggg aggactttga agcaagaatt
     1861 tactccatgc acttgcctca gagtaaggaa aagcggctcc tgaaccggaa cttcatcacg
     1921 aaggatctgg acagctttat tagcagaaat ctgaacgata cccgatatat cagtcgcttc
     1981 ctgaagaatt atatcgagag ttatctgcaa ttctccaacg actcccccaa gagctgtgtg
     2041 gtctgcgtga acggccagtg tacagcccaa ctgcggtcca gatggggact caataagaac
     2101 cgagaagaga gcgatttgca tcacgctctc gatgccgcag tcatagcctg cgcagatcgg
     2161 aagattatca aagagattac caattactac aacgaaaggg agaaccacaa ttataaagta
     2221 aagtatcccc tcccttggca cagttttagg caggacctga tggagacact cgcaggtgtt
     2281 ttcattagtc gcgcgccccg caggaagatt acaggacccg ctcatgacga aaccatacgc
     2341 tctcccaagc atttcaacaa gggcctgaca agcgtaaaga ttccactgac cacagtcaca
     2401 ctggagaagc tcgaaaccat ggtaaagaat actaaaggtg ggatatccga caaggcagtc
     2461 tacaacgtac tcaagaaccg cttgatcgaa cacaacaata aaccccttaa agctttcgcg
     2521 gagaaaatct ataagccatt gaaaaatggc accaacggcg ccataataag aagcattagg
     2581 gtggagactc cgagctacac cggagtgttc agaaatgagg gaaaaggcat ctctgataac
     2641 agcctcatgg tccgcgttga cgtgttcaag aagaaagaca aatactattt ggtccccata
     2701 tacgttgctc acatgatcaa aaaggaactg cctagcaaag ccatcgtgcc tctcaaaccc
     2761 gaaagccaat gggagcttat tgactccacc cacgaattcc tcttctccct gtaccagaat
     2821 gactacctgg taataaaaac caagaagggg atcaccgagg gttattatcg gtcctgtcat
     2881 agaggcacag ggagcctgtc tttgatgcct catttcgcca ataacaaaaa tgtgaagatt
     2941 gacatcggcg taaggacggc tattagcatt gaaaagtata atgttgacat tctcgggaac
     3001 aaaagtattg tgaaggggga accccggagg ggcatggaaa aatacaattc cttcaagtct
     3061 aat
//
