LOCUS       sequence-195217-        3342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-C4ZA16
FEATURES             Location/Qualifiers
     source          1..3342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaactaca ctgagaagga aaagctcttc atgaagtaca ttttggcttt ggacatagga
       61 attgctagtg tcggctgggc catccttgat aaggaatctg agacagtgat tgaagcgggt
      121 tctaatatct tccccgaagc ttctgctgca gacaatcagc tgaggcgaga catgaggggg
      181 gccaagcgga acaaccgcag gctcaaaacc cggatcaatg acttcatcaa actctgggag
      241 aataataatc tttctattcc tcagtttaaa tctactgaaa tagtggggct gaaggtgagg
      301 gcgatcacag aagaaatcac cctcgatgag ctctatctga ttctgtattc ttacctgaag
      361 caccggggga tttcatatct cgaggatgcg ttggacgaca ctgtctctgg atcttccgca
      421 tacgccaatg ggctcaaact gaatgctaag gagttggaaa ctcactaccc ctgtgaaatc
      481 cagcaggagc gcttgaacac aataggaaag tatagaggcc aaagccagat tatcaatgag
      541 aacggcgagg tgctcgacct gagcaatgtt ttcacgatcg gggcttatcg gaaagaaatt
      601 cagagagtat ttgagatcca gaaaaaatac catccagagc tcacagatga gttttgtgat
      661 gggtacatgc tgatctttaa cagaaagagg aaatactatg aaggacctgg aaacgagaag
      721 tcacggacag actacggccg cttcactacg aagctggacg ccaatggcaa ttatattact
      781 gaagacaata tctttgagaa actgatcggc aaatgttccg tttaccccga cgagctcagg
      841 gccgccgcgg ccagctatac cgctcaggag tacaacgtcc ttaatgatct caacaacttg
      901 acgattaacg gtcgaaagct tgaggaaaac gagaagcacg agattgtgga aagaataaag
      961 tccagcaaca caattaatat gcgcaagatt atatccgatt gcatgggcga gaacattgat
     1021 gatttcgctg gagctcggat tgacaagagc gggaaggaaa tcttccataa atttgaagtg
     1081 tacaacaaga tgaggaaggc ccttttggag attggcatcg atatcagtaa ctattcacga
     1141 gaagagctcg atgagattgg ctatataatg actatcaaca ccgacaaaga agccatgatg
     1201 gaagcatttc agaagtcttg gattgatctg tctgacgatg tgaagcagtg tttgattaac
     1261 atgaggaaaa ccaacggcgc cctctttaat aagtggcaat cattcagcct gaagatcatg
     1321 aacgagctca ttcccgagat gtacgcccag ccaaaggaac agatgactct gctgacagaa
     1381 atgggggtca ccaaaggaac ccaagaggag ttcgcaggac tgaagtacat ccctgtcgac
     1441 gtggtgtcag aggacatctt caatcccgtg gtgaggagaa gcgtccggat ctctttcaag
     1501 atccttaatg ccgttcttaa aaaatacaag gctcttgaca ccatagtgat tgaaatgcca
     1561 agagatcgca actcagagga gcaaaaaaag cgaatcaacg attcccagaa actgaacgaa
     1621 aaggagatgg agtatattga aaagaaactg gcagtgacct acggcatcaa gctgtccccc
     1681 agcgatttca gctcccagaa gcaactgagc ctcaagctga agttgtggaa cgagcaggac
     1741 ggaatctgcc tctattccgg aaaaacaatt gatccgaacg atattattaa taatcctcag
     1801 ctgttcgaga tagaccatat cattcccaga tctatctcat tcgacgatgc ccgctccaat
     1861 aaggtcctgg tgtacaggtc cgagaatcaa aagaagggaa accaaactcc atactattat
     1921 cttacgcata gccactcaga atggagcttc gaacagtaca aggcaacagt catgaatctg
     1981 agtaaaaaaa aggagtacgc catttcacgg aagaagatcc agaatctttt gtacagcgag
     2041 gatatcacca agatggatgt acttaagggc ttcattaacc gaaacatcaa cgatacatcc
     2101 tatgccagtc gccttgtact caatacaatc cagaacttct tcatggctaa tgaagctgat
     2161 acgaaggtca aggtgattaa agggtcatat acccaccaga tgagatgtaa cctcaagctg
     2221 gacaaaaacc gcgacgagtc ctacagtcat cacgccgttg atgccatgct catcggatac
     2281 tccgagctgg gatatgaagc ttaccacaag ctccagggtg agtttatcga ttttgagaca
     2341 ggtgaaatac tccggaaaga tatgtgggac gagaatatga gcgacgaggt gtatgccgac
     2401 tatttgtacg gaaagaagtg ggcaaacata cgcaacgagg tggtaaaggc tgagaagaac
     2461 gtgaagtatt ggcattacgt gatgcgcaaa tccaacaggg gcctctgtaa tcagacaatt
     2521 cggggaacac gggagtacga tggcaagcaa tacaagatca ataagctgga tatcaggacc
     2581 aaggaaggta tcaaggtgtt cgcgaaattg gcatttagca agaaagactc tgaccgagaa
     2641 agactgcttg tgtacctgaa tgaccggcgg acattcgacg atctttgcaa gatttatgaa
     2701 gactattccg acgcagccaa cccatttgtt caatacgaaa aggaaactgg agacatcatt
     2761 cggaagtact ctaagaagca caacggcccc cggatcgata agctgaagta caaggatggc
     2821 gaggtggggg cctgtattga catcagtcat aagtacgggt ttgagaaggg cagtaagaag
     2881 gtgatccttg agtctctggt gccctaccgc atggacgtct attacaagga agagaatcac
     2941 tcctactatc ttgttggagt gaagcaatcc gacatcaaat tcgaaaaagg aaggaacgtt
     3001 atagacgaag aggcctatgc tcgaattctg gtgaatgaaa agatgatcca gccgggccag
     3061 agccgagccg acttggagaa cttgggattc aaattcaaac tctcattcta taagaatgac
     3121 atcatcgagt atgagaaaga cggcaaaatc tatacagaaa gacttgtctc ccggacgatg
     3181 cctaagcaac gcaactatat tgaaaccaag cccattgaca aggctaagtt cgaaaagcag
     3241 aaccttgtgg ggctgggcaa aactaagttc atcaaaaagt atcgatacga catcctcggg
     3301 aataagtatt cttgtagtga ggagaagttt acgagcttct gt
//
