LOCUS       sequence-195219-        3420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-C7G697
FEATURES             Location/Qualifiers
     source          1..3420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagagaga atggatccga cgagcggagg cggaatatgg acgagaaaat ggactaccgg
       61 ataggacttg atattggaat agcatctgtg gggtgggccg tgttgcagaa taactccgac
      121 gatgaaccgg taagaatcgt agatttgggg gtgagaattt tcgacacagc agaaatccca
      181 aagaccggcg aatccctggc aggaccccgg cgcgcagcgc gcaccactcg aaggagactg
      241 agacgccgaa aacatagact tgacaggatc aaatggctgt tcgaaaacca ggggctgatc
      301 aacatcgacg atttcctgaa gagatataac atggcaggcc tccctgacgt gtatcagctg
      361 aggtacgaag ctctggaccg aaagctgacc gatgaggaac tggcccaagt ccttctgcac
      421 atcgccaagc atcgggggtt taggtccaca cgaaaagcag aaacagctgc taaagaaaat
      481 ggcgcagtgc tgaaggccac agatgaaaac cagaaacgca tgcaagagaa ggggtaccgg
      541 actgtgggag aaatgattta tctcgacgaa gcctttcgca cggggtgctc ttggtccgag
      601 aaaggctaca tccttacccc acggaacaaa gcggagaatt atcagcatac catgctgagg
      661 gcaatgctcg ttgaggaggt aaaggaaatc ttctcctctc agaggcgatt gggtaacgaa
      721 aaggccacag aagagctcga agaaaagtac ctcgaaatca tgacctcaca gaggtctttt
      781 gatctgggcc ctggcatgca gcctgacggt aagccctcac cgtacgccat ggaagggttc
      841 tcagataggg tcggaaagtg caccttcctg ggggaccagg gcgagctgag aggcgcaaag
      901 ggaacataca ctgctgaata cttcgtggcc ctgcagaaaa ttaaccacac caaactcgtt
      961 aaccaggacg gagagacaag aaattttact gaggaggaac ggcgggcgct cactttgctt
     1021 ctctttactc aaaaggaagt gaaatatgcc gcggtgcgga agaagctggg tctgcccgaa
     1081 gacatcctct tttacaatct caactataag aaggccgcca ctaaagaaga gcagcagaaa
     1141 gagaaccaaa atacagaaaa ggctaagttc atcggaatgc cctattatca tgattataag
     1201 aaatgcctgg aggagcgggt aaagtacctg acagagaatg aagtgcgcga cctgtttgat
     1261 gagatcggga tgatcctgac ctgctacaag aatgatgact cccggactga acgacttgct
     1321 aagctgggtt tggtgcctat cgagatggag ggattgctgg cctatacccc aacgaaattt
     1381 caacacctgt ctatgaaggc gatgagaaac attattccat ttctcgagaa gggtatgacc
     1441 tacgacaagg cctgtgaaga ggccggatac gatttcaagg cagatagcaa aggaactaaa
     1501 caaaagctcc tgactgggga aaacgtaaat cagaccatca atgagatcac taaccccgtg
     1561 gtcaagaggt ccgtgtctca gactgtgaag gtgatcaacg ccattattcg gacatatggg
     1621 tccccgcaag ccattaacat agagctggcg cgggagatgt ctaagacttt tgaggagagg
     1681 agaaagatca agggtgatat ggagaaaagg cagaagaaca acgaggacgt gaagaaacag
     1741 attcaggaac tggggaagct gtctccaacc ggacaggaca ttctgaagta ccggctctgg
     1801 caagaacagc agggcatctg tatgtatagc ggaaagacca tccccttgga agagctcttt
     1861 aagccaggat atgacattga tcacatcctg ccttactcca ttaccttcga cgactcattc
     1921 cgcaacaagg tactcgtgac aagccaggag aacaggcaaa aagggaacag gacaccatat
     1981 gaatacatgg ggaatgacga acagcgctgg aatgagtttg aaaccagggt taagactacc
     2041 attagagact acaagaagca gcaaaagctg ctgaagaagc attttagcga ggaggaaagg
     2101 tccgaattca aggagcggaa ccttacagac acaaagtaca ttaccaccgt gatctataat
     2161 atgatcagac aaaatctgga aatggctccc ctcaaccgcc ctgaaaagaa gaagcaggtt
     2221 cgcgccgtta atggagccat cacagcttac ctgaggaaaa gatgggggct gccacaaaag
     2281 aatcgggaaa cagatacaca tcacgctatg gatgcagtgg ttatcgcctg ctgtacggat
     2341 ggtatgatcc agaagattag ccgctacact aaggtacgag agaggtgcta cagtaaaggg
     2401 actgagtttg tggacgcaga aaccggtgaa attttccggc cagaggatta ttctagggca
     2461 gagtgggatg aaatattcgg agtgcatatc ccaaagccat gggaaacttt tagagccgaa
     2521 cttgacgtca gaatgggaga cgaccccaag ggtttcctgg atacacatag tgacgttgcc
     2581 ctggagctcg actacccaga gtatatttat gagaacctgc gacctatctt cgtgtccaga
     2641 atgccgaatc acaaggtgac cggggcagcc catgcagata ccatcagaag cccgcggcac
     2701 tttaaggatg aggggatagt tctgaccaag actgctctga cagacctgaa gttggataaa
     2761 gacggtgaaa ttgatgggta ttacaacccg cagtctgacc tcctgcttta tgaggccttg
     2821 aagaagcaac ttctcctgta tggtaatgac gccaagaagg ctttcgccca ggacttccac
     2881 aagccgaaag cggacggcac agaggggcct gtcgtccgga aagtaaagat ccagaagaag
     2941 cagactatgg gggttttcgt tgatagtggc aacgggatcg cagagaatgg cggaatggtt
     3001 agaatcgatg tgtttcgagt gaacggcaag tactacttcg tgcccgtgta caccgccgac
     3061 gtggtgaaga aagtactgcc caatcgcgcc tccacggctc acaagcctta tggagagtgg
     3121 aaagtgatgg aggataaaga tttcctgttt tctctgtact cccgggactt gatacacatt
     3181 aaatcaaaga aggacatacc cattaagatg gtgaatggcg ggatggaagg gatcaaggag
     3241 acttacgcgt actatatagg agcagatatc agcgcagcga acatccaagg catcgcccac
     3301 gactcacggt acaaattccg ggggttgggg atccagagcc tcgatgtcct ggaaaagtgc
     3361 cagatcgacg tgttgggcca cgtcagtgtg gtgcggtccg agaaacgcat ggggttcagc
//
