LOCUS       sequence-19522-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF39
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(232..249)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    238..462
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(595..614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggctactaga gaaccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctagcgttt aaacgggccc tctagacccg gggagaagaa atttgcctgc
      121 cctgagtgtc ctaagcgctt catgacaagc gccaacctga gccgtcatat caagacccac
      181 accggtggat cctctgcaca gtagtaagct taagtttaaa ccgctgatca gcctcgactg
      241 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      301 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      361 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      421 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
      481 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
      541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      721 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      781 cgttggagtc cacgttcttt aatagtggac tcttg
//
