LOCUS       sequence-195221-        4089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-C7TEQ6
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacaaagc tgaaccagcc ctatggaatc ggcctcgata ttggaagtaa tagcatcggc
       61 ttcgccgtgg tagatgcgaa ctcacacctg cttcgcctga aaggggagac tgccatcgga
      121 gctaggctgt ttcgcgaagg gcaaagtgca gccgatcgga gaggtagccg cacaacaagg
      181 agaagactca gtcgaactag atggaggctg tccttcctga gggacttttt cgccccacac
      241 ataaccaaga ttgatcctga ttttttcctg agacagaagt acagcgaaat cagccccaaa
      301 gataaggacc ggtttaagta tgaaaaaaga ttgtttaatg acagaacaga cgcggaattc
      361 tacgaggatt atcccagcat gtaccatctt aggctccacc ttatgacaca cacacataag
      421 gctgatcccc gcgagatttt cctcgccatt caccatattc tgaagtctcg agggcatttc
      481 cttacccccg gggcggccaa agacttcaac accgataaag tggaccttga ggacattttc
      541 cctgccctga cagaagcata cgcgcaggtg tatcccgacc tggagttgac ctttgatctg
      601 gctaaggccg acgatttcaa ggcaaagctg cttgatgaac aggcgacacc cagcgacact
      661 cagaaggcgc tcgtcaattt gcttctgtca agcgacgggg aaaaagaaat cgtcaagaag
      721 agaaagcagg tcttgaccga attcgctaag gctatcaccg gacttaagac aaaattcaac
      781 cttgcactgg ggaccgaagt cgatgaggcc gatgcttcta actggcagtt cagcatgggc
      841 cagcttgacg acaaatggtc taatattgag acttctatga ctgaccaagg gaccgaaatt
      901 tttgaacaga tccaggagct ctatcgcgca aggctgctga atgggattgt gcctgccgga
      961 atgtctctgt cccaggccaa agtggctgac tacgggcagc ataaggagga tctcgagctc
     1021 ttcaagacct atctgaagaa actgaacgat cacgaactcg ctaaaaccat ccgcggcctc
     1081 tacgacaggt acatcaacgg ggatgacgct aagcccttcc tcagagaaga ctttgtgaaa
     1141 gccctgacaa aagaggttac agcgcatcca aatgaggtaa gcgagcagct gctgaatcga
     1201 atgggacagg ctaactttat gctgaagcag cgaaccaaag ccaacggggc gatcccaatc
     1261 cagctgcaac agagggagct ggaccagatt atcgcgaatc agagtaagta ctacgattgg
     1321 cttgcagccc caaacccagt cgaggcacac agatggaaga tgccttatca gctggatgaa
     1381 cttctgaatt tccacattcc atactatgtc gggccattga tcacaccgaa gcagcaggct
     1441 gagtccgggg agaacgtctt tgcttggatg gtgcgcaagg atccttcagg caatataacc
     1501 ccctacaatt tcgacgagaa ggttgataga gaagcctccg ctaatacatt tatacagcgg
     1561 atgaaaacca cagataccta tctgattggc gaagatgtac tccccaaaca gtccttgctg
     1621 tatcaaaagt atgaggtgct taacgagctg aataatgtaa ggattaataa tgagtgcctt
     1681 ggcaccgacc aaaagcagcg actcatccgg gaagtgttcg aacggcacag ttcagtgacc
     1741 atcaaacagg tggcggacaa cctcgtagca catggcgact tcgcacgcag gcctgaaatc
     1801 cgcggccttg cagatgaaaa gcgctttctc tcatctctgt caacatacca ccagctgaaa
     1861 gagattctgc acgaagcgat tgatgaccca actaaacttc tggatataga gaatattatc
     1921 acctggtcta cagtgttcga ggatcatact atcttcgaga caaaactggc tgagatcgag
     1981 tggttggacc ccaaaaagat caatgaactg agtggaatca ggtatcgagg atgggggcaa
     2041 ttcagccgga aactgcttga cggtctgaag ctgggaaacg ggcatacagt gattcaggag
     2101 ctcatgctct ccaatcataa tctcatgcag attctggcag acgaaacgct caaagaaaca
     2161 atgacagaac tcaaccagga caagctgaaa actgatgata tagaggatgt gataaacgac
     2221 gcatacacaa gtccaagtaa taaaaaggcc cttcgccagg tactcagagt ggtggaggat
     2281 attaagcacg ccgccaacgg ccaagaccct agctggctgt ttatcgagac tgctgacggg
     2341 actggtaccg ctggtaagcg gacccaaagt cgacagaaac agatacagac cgtctacgca
     2401 aacgccgccc aggaattgat tgattcagct gtcagaggag agctcgagga caagatcgcc
     2461 gataaggcat cttttacgga taggcttgtc ctctatttca tgcagggcgg acgcgacatc
     2521 tacacgggcg cacccctcaa cattgaccaa ctgtctcatt acgatatcga ccatattctg
     2581 cctcaaagcc tgattaaaga tgattccctt gacaaccgag tcctcgtcaa tgccactatt
     2641 aatcgggaaa aaaataacgt gtttgcatca acattgttcg ccggtaaaat gaaggctaca
     2701 tggcggaaat ggcatgaagc cggactgatt agtggaagga agcttcggaa tctgatgctc
     2761 cgaccagatg aaatcgataa atttgcaaag ggattcgtag ctcgccaact ggtggagact
     2821 cgacagataa ttaaattgac cgaacaaatt gccgctgcgc agtacccaaa tactaagatc
     2881 atcgcagtca aggcaggtct gagtcatcag ctgcgcgagg agctcgactt cccaaaaaac
     2941 cgggatgtaa atcactatca tcatgctttt gacgcctttc tcgccgcccg aatagggacc
     3001 tatctcctga aaagataccc caaactcgct cctttcttca cttacggcga gtttgcgaag
     3061 gttgacgtca agaagttccg ggaattcaac ttcattggcg cactcacaca cgctaagaag
     3121 aatataattg ccaaggacac cggggaaatt gtgtgggaca aagagaggga catacgggag
     3181 ctggaccgaa tctataattt caagaggatg cttattacac acgaggtata ttttgaaacc
     3241 gctgacttgt tcaagcagac gatctacgcc gccaaagact ctaaagagcg gggaggttcc
     3301 aaacagctca ttcccaagaa gcaaggctac cctactcagg tgtacggtgg gtatacccag
     3361 gagtctggaa gctacaacgc actggtgcga gtggcagaag cagatacaac cgcctatcag
     3421 gttatcaaga tcagcgcaca gaatgccagc aaaatcgcat ctgcaaacct gaaatcccgg
     3481 gaaaagggta agcagctgtt gaacgaaata gtcgtgaagc agctggccaa gagacgcaag
     3541 aactggaagc cttctgcgaa ttctttcaag atcgtgatcc ctcggttcgg aatgggaaca
     3601 ctcttccaaa acgccaagta tggcctgttt atggtgaaca gtgacacgta ctataggaat
     3661 tatcaggagc tgtggctgag ccgagagaac cagaaactcc tgaaaaaact tttctctatc
     3721 aagtacgaaa agacccagat gaatcatgac gctcttcagg tgtataaagc aattatcgac
     3781 caagtggaga aattctttaa gctgtatgat attaatcagt ttagggctaa actgtccgat
     3841 gcgatcgagc ggttcgagaa gctccctatc aacaccgacg gaaataaaat cgggaagacc
     3901 gaaaccttgc gccagatact cattggcctt caagccaacg gaaccaggag caatgtcaaa
     3961 aacttgggca ttaagacgga tctggggctc cttcaggtgg ggagtggcat caagctcgac
     4021 aaagacacac agattgtgta ccagtctcct agtgggctgt tcaagcgcag aattcccctc
     4081 gccgatctg
//
