LOCUS       sequence-195223-        3654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-D1VXP4
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaacaaaa gaattcttgg cctggacacc ggcaccaatt cactcgggtg ggcagtcgtg
       61 gactgggatg agcacgctca gtcttacgaa ctcatcaagt atggagatgt gatcttccag
      121 gaaggggtca agatcgagaa ggggatagaa tcttctaagg ctgccgaaag gagcgggtac
      181 aaggcaattc ggaaacagta tttcaggcga cgactcagaa aaatacaagt gctcaaggtt
      241 ctggtaaagt accatttgtg tccatatctc tccgacgatg acctccggca atggcatctc
      301 cagaagcagt accccaaatc agatgaactc atgctgtggc agagaacgtc agatgaggaa
      361 gggaagaatc catattatga tcgccacaga tgtctgcatg agaagctgga tctgactgtt
      421 gaagcggacc gctataccct tggccgcgcc ctgtaccacc tgacgcagag gcgcgggttc
      481 ctttcaaatc ggctggacac atccgccgat aataaggagg atggtgtggt gaagtccggt
      541 atctcccagt tgtctaccga aatggaggag gcaggatgtg agtacttggg ggattacttt
      601 tacaaacttt atgacgctca gggcaataaa gtgaggattc ggcaaagata cacagatagg
      661 aacaaacatt atcagcatga gttcgatgcg atctgtgaaa agcaagaact gagcagtgag
      721 cttattgagg atctgcaacg ggccatcttc ttccaactcc cattgaaaag tcagcgccac
      781 ggggtgggac ggtgcacctt cgaaaggggg aaaccaagat gtgcagactc tcacccagat
      841 tacgaagaat ttcggatgtt gtgctttgtg aataacatcc aggtaaaagg gccgcacgac
      901 cttgagctgc ggccgttgac ttacgaggaa cgggaaaaaa ttgaaccctt gttcttccgc
      961 aaatcaaagc caaactttga tttcgaagac attgccaagg cacttgccgg taaaaagaac
     1021 tatgcgtgga tccacgataa ggaagaaaga gcctacaaat ttaactacag gatgactcaa
     1081 ggagtgcctg ggtgccccac tattgcacag ctgaaatcca tcttcggaga tgactggaag
     1141 actgggattg cggagacata taccctgatc cagaaaaaaa atggcagcaa gagcctgcaa
     1201 gaaatggtgg atgacgtgtg gaacgttctc tactcctttt ctagtgttga gaagctgaag
     1261 gagttcgcgc atcataaact gcagctcgac gaagaatcag ctgagaaatt cgcaaaaatc
     1321 aagctctctc attcatttgc tgcgctcagc ctgaaggcca tccgaaagtt tctccccttt
     1381 ttgcgcaagg gaatgtacta cacacatgcc tcattcttcg ctaacatccc aaccatcgta
     1441 ggcaaggaaa tatggaacaa ggagcagaac cggaagtaca tcatggaaaa tgtgggcgag
     1501 ctcgttttca actatcagcc taaacaccgg gaggtccagg gtaccatcga aatgctcata
     1561 aaagattttc tcgctaacaa ttttgagctg ccagctggcg caactgacaa actgtatcac
     1621 ccctctatga tagaaaccta ccccaacgca cagcgcaatg agttcgggat cctccagctc
     1681 ggcagccccc gcaccaatgc tataagaaat cccatggcta tgaggagcct ccacatcctg
     1741 cgaagagtgg tgaatcagct gctgaaagag agcatcatcg acgaaaatac ggaggtgcac
     1801 gtagagtacg ccagggaact caatgacgct aacaagcgga gggcaattgc tgacagacag
     1861 aaagaacagg ataaacaaca caaaaagtac ggagacgaaa ttaggaagct ttacaaggaa
     1921 gaaaccggca aggacattga accaacccag acagatgttc tgaaattcca gttgtgggag
     1981 gaacagaatc accattgtct gtacacaggc gagcagatag gcatcacaga ttttataggg
     2041 tctaatccta aattcgacat cgagcatacc ataccacagt ccgtcggagg tgatagtact
     2101 cagatgaatt tgactctgtg tgacaaccgg ttcaatagag aagtgaaaaa ggctaaactg
     2161 cccaccgaac ttgccaatca cgaggagatt ctcacccgga tcgaaccttg gaaaaataaa
     2221 tacgaacaat tggtgaaaga acgagacaag cagcggacct ttgcaggcat ggacaaggca
     2281 gttaaggata ttcgcattca gaagcggcac aagctccaaa tggagatcga ttattggcgc
     2341 ggtaagtatg aaagatttac gatgaccgag gtgccagagg gtttcagtag aagacaagga
     2401 actggaatag gtcttatttc aaggtacgcc ggactctacc tcaagagtct tttccaccaa
     2461 gctgactcta ggaacaagtc taacgtgtat gttgtcaagg gcgtcgcgac ggcagagttc
     2521 cggaagatgt ggggcctgca atctgagtat gaaaaaaagt gtcgggacaa tcactcacac
     2581 cactgtatgg atgctatcac catcgcttgc atcggtaagc gcgaatacga cctgatggcc
     2641 gagtactatc ggatggagga gacatttaaa cagggtcgag gttctaaacc aaagtttagc
     2701 aaaccgtggg ccaccttcac agaggacgtc ctcaacatat acaagaacct tctggttgtg
     2761 cacgatacgc ctaacaatat gccgaaacac acgaagaaat acgtccagac atctatcgga
     2821 aaggtccttg cccagggaga cacagcccgc ggctccttgc atctcgatac ttattacggc
     2881 gctatagaga gagacggcga gatacgctat gtcgttcgcc gacctctgag tagttttact
     2941 aagcctgaag aattggagaa tattgtagac gaaaccgtaa agaggactat aaaagaggct
     3001 atcgccgaca aaaatttcaa gcaagcaatt gcagaaccca tctacatgaa cgaagagaag
     3061 ggtattctga taaagaaagt gcgatgcttt gccaaaagtg tgaagcagcc tatcaacatc
     3121 cgccagcaca gggacctctc caagaaagaa tataaacagc aataccacgt tatgaacgaa
     3181 aataattatc tgttggctat ttacgaaggt ctggtgaaga ataaagttgt gcgagagttc
     3241 gaaatcgtgt cttacatcga ggccgctaag tactacaagc ggtcacagga tcggaacatt
     3301 tttagttcta tcgtgccaac tcatagcacc aaatacggcc tccccctgaa gacgaagctg
     3361 ctgatggggc agcttgtgct tatgtttgag gaaaacccgg acgagatcca agtagacaat
     3421 acaaaagacc tggtcaaacg attgtataaa gtggttggga tcgagaaaga cggccggatt
     3481 aaatttaaat atcaccaaga ggctaggaag gaagggctgc ctatcttctc tactccatat
     3541 aagaataacg atgattacgc ccctatcttc cgacagtcaa tcaataacat aaatattctg
     3601 gtcgacggaa tcgacttcac tattgatatt ctgggcaagg tgactttgaa agag
//
