LOCUS       sequence-195227-        3072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-D3UFL8
FEATURES             Location/Qualifiers
     source          1..3072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgattcgaa ctctgggcat cgatatcgga attgcctcaa ttggatgggc cgtcattgag
       61 ggcgagtata cagataaggg gctggaaaac aaggaaatcg ttgcttctgg cgtgcgggtg
      121 tttacgaagg cagagaatcc aaagaataaa gagagcctcg ccttgcctcg gaccctggca
      181 cgcagtgcaa gaaggcggaa cgctagaaag aaagggcgga tccagcaggt gaaacactac
      241 ctgtcaaaag cccttggcct cgacctcgag tgtttcgtgc agggagagaa gctggccact
      301 ttgtttcaga cttccaagga ttttctgagc ccatgggaac ttcgggaaag agcgttgtac
      361 cgggtcctgg ataaagagga gctggcccga gtaatactgc atattgccaa acgcaggggc
      421 tatgatgaca tcacctatgg ggtcgaggat aatgactcag gcaagattaa gaaggcaatt
      481 gccgaaaaca gcaagaggat aaaagaagaa caatgtaaga ctatcgggga gatgatgtac
      541 aagctttatt ttcagaaaag tcttaatgta aggaataaga aagaaagtta caataggtgc
      601 gtgggtagaa gtgaacttag agaagaactg aagaccattt tccaaattca gcaggagctg
      661 aaatcccctt gggtgaacga ggaacttatt tacaaattgc ttggaaaccc tgatgcccag
      721 tccaagcagg agcgggaggg actgattttc tatcagcgcc ctcttaaagg cttcggcgac
      781 aaaattggaa aatgcagtca catcaagaag ggcgagaata gcccctatcg cgcctgcaag
      841 cacgctcctt ccgccgagga gttcgtggcc ctcacaaagt caatcaattt cctcaagaat
      901 cttacaaacc ggcatggcct ctgcttttca caggaggaca tgtgcgttta cctggggaaa
      961 atcctccagg aggcccaaaa gaacgagaaa ggactgacat actccaagct caagcttctt
     1021 ctggatcttc cctctgattt tgagtttctg ggcttggatt atagtggcaa gaaccccgag
     1081 aaggccgttt tcctctccct gccctctact tttaagctga ataaaatcac ccaagatcgg
     1141 aaaacacaag acaaaatcgc caacatcctc ggcgcgaata aagactggga ggccatcctt
     1201 aaggaactgg aatctctgca gttgtctaag gaacagatcc aaactattaa ggacgccaaa
     1261 ctcaatttta gcaagcacat taatctgtcc ctcgaagcac tctaccacct tctccctctg
     1321 atgcgcgaag ggaagagata tgatgaaggc gtggaaatcc tgcaggagcg cgggatattc
     1381 tccaagcctc agcctaagaa ccgacagctt ctccctcctc tgtcagagtt ggctaaggag
     1441 gaatcttatt ttgatatccc taatccagtg ttgcgcaggg cccttagtga gttccgaaag
     1501 gtggtaaacg cccttcttga aaagtacgga gggttccatt attttcacat tgaattgacg
     1561 cgcgatgtct gtaaggctaa gtcagcacgc atgcagctgg aaaagataaa caagaagaac
     1621 aagtctgaaa atgacgccgc cagtcagctg ttggaggtct tggggcttcc caacacctac
     1681 aataatcgct tgaagtgtaa gctgtggaaa cagcaggaag agtattgtct ctactccgga
     1741 gaaaagatta ccatagatca cctgaaggat cagcgcgccc tccagatcga tcacgcattt
     1801 cccctgagtc gatccctcga cgactcacag tcaaacaagg tgctgtgtct gacttcctca
     1861 aatcaggaga agagcaataa gaccccatac gaatggctgg gaagcgacga gaagaagtgg
     1921 gacatgtacg ttggccgggt gtactcctcc aacttctccc cttccaagaa gaggaagttg
     1981 acgcagaaga atttcaaaga gcgcaatgaa gaagatttcc tcgcccgcaa cttggtcgac
     2041 accggttata tcggccgagt tactaaggag tatattaaac attccctcag tttcctgcca
     2101 ctgccggatg gtaagaaaga gcatattagg atcatttcag gctccatgac atctacaatg
     2161 cggtcatttt ggggtgttca ggaaaagaat cgagatcacc acttgcatca tgcacaggac
     2221 gcaatcatca ttgcatgtat cgagcccagc atgattcaaa agtataccac gtatctcaaa
     2281 gataaggaaa cgcacaggtt gaagtcccac cagaaagccc agatactgcg agagggtgac
     2341 cacaaattgt cactgaggtg gccgatgagc aatttcaaag ataaaataca ggagtccatt
     2401 cagaatatca tcccatcaca ccacgtgagc cacaaggtca ccggtgaact ccaccaggag
     2461 actgtgagaa ccaaagagtt ctactaccaa gcctttggag gggaggaagg agtgaagaaa
     2521 gcactgaagt tcggaaagat cagggagatc aatcagggaa tcgtagacaa cggggctatg
     2581 gtgcgggtgg acatcttcaa gtccaaggac aaggggaaat tctacgcggt tccgatctac
     2641 acatacgact ttgccattgg caagcttcca aacaaggcaa tcgtgcaagg gaagaagaac
     2701 ggcatcataa aggattggtt ggagatggat gagaactatg aattctgttt cagtttgttc
     2761 aagaatgatt gcatcaagat acagactaaa gagatgcagg aggccgtgct tgcaatatat
     2821 aagtcaacaa atagtgcaaa agctacaata gagctcgaac acctgagtaa gtatgccctg
     2881 aagaacgaag acgaggagaa aatgttcact gacaccgaca aggagaagaa taagaccatg
     2941 actcgggaga gttgcgggat tcaagggctc aaggttttcc agaaagtgaa actctcagtg
     3001 ctgggtgagg ttttggaaca caaacctcgc aacagacaga atatcgccct gaagacaaca
     3061 cccaaacatg tc
//
