LOCUS       sequence-195229-        4014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-D4J3S7
FEATURES             Location/Qualifiers
     source          1..4014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagcaag aatacttttt gggactggac atgggcaccg gcagcctcgg ctgggcagta
       61 acagattcca cttaccaagt gatgagaaaa cacggaaagg ccctttgggg cacccgactc
      121 tttgagagtg caagtacagc cgaagagagg cgaatgttcc ggacagcaag gagacggttg
      181 gatcgacgaa actggcgaat acaggtactt caggaaatct tcagcgagga aataagtaag
      241 gtggatcctg ggttcttcct gagaatgaag gagagtaagt actacccaga ggataaaagg
      301 gacgcagaag ggaattgccc agagctgcca tatgctctct ttgtggatga taattatact
      361 gataagaatt atcataaaga ctacccaacc atctaccatc tgcgcaaaat gcttatggaa
      421 accactgaga ttccagatat caggctcgtc tatttggttc ttcatcatat gatgaagcac
      481 cggggacact tcctgctgtc tggggatatc tctcagatca aggagtttaa gtctacattc
      541 gaacagctga tccagaacat ccaggatgag gagcttgagt ggcatattag cttggacgat
      601 gccgccattc agtttgtgga gcatgtactg aaagacagga atctcacgag atctaccaag
      661 aagagccggt tgataaagca gcttaacgca aagagcgcat gcgaaaaagc tatccttaat
      721 ctcctgtccg ggggcactgt caaactgtca gacatattta ataataagga gctggacgag
      781 tccgaaagac caaaagtcag cttcgccgac tctggttatg acgattacat cggcatagtt
      841 gaggctgaac tggccgagca gtattatatt atcgccagcg caaaagccgt gtatgattgg
      901 agcgtgctgg tggaaattct cggcaactcc gtgagtattt ctgaggcgaa gatcaaggtg
      961 taccagaaac atcaggctga cttgaagact ctgaaaaaga ttgttaggca atacatgact
     1021 aaggaagatt acaaaagggt gttcgtcgac accgaagaaa aactgaacaa ttattcagcc
     1081 tatatcggaa tgactaaaaa gaatggaaag aaggttgatc ttaagagtaa acagtgtacc
     1141 caggccgact tttatgattt tttgaaaaag aacgttatca aggtgatcga tcataaagaa
     1201 atcacacagg agatagaatc cgaaatcgag aaggaaaact tcctccccaa gcaggtgacc
     1261 aaggataatg gggttatccc ataccaggtt cacgattatg agctcaagaa gatactggac
     1321 aatttgggta cacgcatgcc atttatcaaa gaaaacgctg aaaagatcca gcagctgttc
     1381 gagtttcgga tcccctatta cgttggacct ctgaaccgag ttgacgacgg caaggacgga
     1441 aaatttacat ggtccgtgag gaaatctgac gccaggatct atccctggaa cttcactgag
     1501 gtcattgatg ttgaggcctc cgcggagaag tttatacgga ggatgacaaa caagtgcaca
     1561 tatctcgtcg gcgaagatgt tcttcccaaa gattcacttg tgtacagtaa gttcatggtg
     1621 ctcaacgaac ttaacaactt gagactgaac ggagagaaga taagcgtgga gctcaagcaa
     1681 aggatctacg aggaactgtt ttgcaagtat cgcaaagtga ccaggaaaaa gctggagcgg
     1741 tatctcgtga tcgaggggat tgcaaagaag ggcgtggaga tcactgggat cgatggggat
     1801 tttaaggcaa gccttaccgc ttatcacgat ttcaaggagc gcctgacaga cgtgcagctg
     1861 tcccagagag ccaaggaggc tatagtgctt aacgtggtgc tgtttgggga cgacaagaaa
     1921 ctcctcaaac agcggttgtc aaagatgtac cccaatctca ctaccggaca gctgaaaggc
     1981 atttgcagcc tgtcctatca gggatgggga cgactttcca aaacgtttct ggaggaaatt
     2041 accgtaccag cccccggtac gggcgaagtt tggaacataa tgaccgcact ttggcagacc
     2101 aatgacaacc tcatgcaact cctgagtcgc aattacggtt ttacaaacga ggtggaggag
     2161 ttcaacactc tgaagaaaga aaccgacttg agctacaaaa cagtggatga gctttacgtt
     2221 agccctgccg tcaaacggca aatctggcag accctcaaag tagtgaaaga gatacagaag
     2281 gttatgggaa atgcaccgaa gcgggttttt gtggaaatgg ctcgagagaa acaggagggc
     2341 aagaggtccg attctagaaa aaagcagctt gttgaactgt accgcgcttg taaaaacgag
     2401 gagagagact ggatcactga actgaacgct cagagcgatc aacagcttag gagtgacaag
     2461 ctgttcctgt actacattca gaagggtaga tgcatgtact ccggggaaac cattcagctg
     2521 gacgaattgt gggataatac caaatatgat attgaccata tctatcctca gtcaaagaca
     2581 atggacgatt ccctgaataa ccgggtcctc gtaaagaaaa attacaacgc catcaagagc
     2641 gatacctatc ctctgagcct tgatatccag aagaaaatga tgtccttctg gaaaatgctt
     2701 caacaacagg gcttcattac gaaggaaaag tatgtgcggc ttgtgcggtc tgacgagctg
     2761 agcgctgatg aattggccgg cttcatagag agacaaattg tggaaaccag acagagtacc
     2821 aaggcggttg cgacgatcct taaggaggct ctgcccgaca cagagattgt gtacgtaaag
     2881 gctgggaatg tttctaactt ccggcagacc tatgagctgc tgaaggtgcg cgaaatgaac
     2941 gatctccacc acgctaagga cgcatacttg aatatagtgg tggggaacgc gtacttcgtg
     3001 aagtttacga aaaacgctgc gtggtttatc cgcaacaatc caggccgctc ttataacctg
     3061 aaacggatgt tcgagttcga tatcgagcgg tctggtgaaa tagcttggaa agccggtaat
     3121 aagggcagta ttgtcactgt taagaaggtg atgcaaaaga ataatatcct tgtgacaagg
     3181 aaagcctatg aggtaaaagg cgggctgttc gatcagcaga ttatgaaaaa aggcaaggga
     3241 caggtcccga tcaagggtaa tgatgagcga ctggctgaca tcgagaagta cgggggctat
     3301 aataaggcgg ccgggactta tttcatgctg gtgaagtctc tggataagaa aggaaaggaa
     3361 attcgaacta tcgagttcgt accactgtat ctgaagaacc aaatcgaaat caatcatgag
     3421 tccgccattc agtacctggc tcaggaaaga ggactgaata gcccagaaat tctcctttcc
     3481 aagatcaaga tcgacactct gtttaaggtc gacggattca agatgtggct ctcaggacgg
     3541 acagggaatc agctgatctt taagggcgca aaccagctga ttctttcaca ccaggaagct
     3601 gcgatcctga aaggtgtggt gaagtacgtg aataggaaga acgagaacaa agatgccaag
     3661 ctgtctgaac gagatgggat gaccgaggag aaactgcttc agctgtacga caccttcctg
     3721 gacaagctgt ctaacacggt ttatagtatt cgactctccg cacagatcaa gactctgaca
     3781 gagaagaggg caaaattcat cgggctgtcc aatgaagacc agtgcatagt tctcaacgag
     3841 atcttgcata tgtttcagtg tcagtccggc agcgccaatc ttaagctcat agggggcccc
     3901 ggcagcgctg gtatcctggt tatgaataac aatatcaccg cttgcaaaca gatttcagtg
     3961 atcaatcagt cacccaccgg catatatgaa aaggaaatcg acctgatcaa actg
//
