LOCUS       sequence-195231-        4095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-D6GRK4
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacaaaag aatactattt gggtttggac gtgggaacaa acagcgtagg gtgggccgtg
       61 actgatagcc agtataacct ttgcaaattt aagaagaaag acatgtgggg cattcggttg
      121 ttcgaatcag ccaacacagc aaaagacaga agactgcaaa ggggcaaccg aaggcgcctg
      181 gagaggaaga aacaaagaat tgacttgctc caggaaatct tctcccccga aatttgcaag
      241 atcgacccta cttttttcat tcgcctcaat gagtcccgcc ttcatctgga ggacaaaagc
      301 aacgatttta agtaccctct cttcattgag aaagactact ccgacataga gtactataag
      361 gaattcccaa caatattcca tctgcggaag cacctcatag aatccgaaga aaagcaggac
      421 atccgcctga tttatctggc tctccataat atcattaaaa cacgcggtca cttcctgatc
      481 gacggcgact tgcagtccgc gaaacagctc cgacctattc ttgatacttt cctgctgtcc
      541 ctgcaggaag agcaaaacct cagcgtgagc ctcagcgaga accagaagga cgaatacgag
      601 gagatcctga aaaacagaag catcgccaaa tcagaaaagg tgaagaagct gaaaaatttg
      661 ttcgaaattt ccgacgagtt ggaaaaggag gaaaagaagg cgcagagtgc cgtcatcgag
      721 aatttttgca agtttatagt ggggaacaaa ggagatgtct gcaagttcct gagggtcagt
      781 aaggaggagc ttgaaatcga ctctttttca ttttcagagg gaaagtacga ggatgacatt
      841 gtgaagaacc ttgaagaaaa agtgccagag aaggtgtacc tcttcgagca gatgaaagcg
      901 atgtacgact ggaacatcct ggtggatatt ctggaaaccg aggaatatat ctcctttgcc
      961 aaagtgaagc agtatgagaa gcataagacc aacctccgcc tcctcaggga tatcattctt
     1021 aagtattgta cgaaggatga atataaccgg atgttcaacg acgagaagga ggctggctct
     1081 tacacggcat acgtggggaa actgaagaaa aacaataaga aatactggat tgaaaagaag
     1141 cgcaaccctg aagagttcta taaatctctg gggaagctgt tggacaagat cgagcccctg
     1201 aaggaggatc tggaagtgct gacaatgatg atagaagagt gcaagaatca caccctgctg
     1261 cccatccaga agaataagga caacggggtc atcccgcatc aggttcacga agtcgagctt
     1321 aagaaaattc tggagaacgc aaaaaagtac tactcttttc ttactgaaac agataaagac
     1381 ggatactcag ttgtccagaa aatcgagtcc atctttcggt tccggatccc ttactatgtt
     1441 ggccctctga gcaccaggca ccaagagaag ggtagtaatg tttggatggt gcgcaagcct
     1501 gggagggagg accgcatcta cccgtggaac atggaagaga tcattgactt cgagaagtcc
     1561 aacgagaact tcattaccag aatgacaaac aagtgcacat accttattgg cgaggacgtc
     1621 ttgccaaagc atagccttct gtactccaaa tacatggtgc tgaacgagct gaataatgtg
     1681 aaggtgcggg gaaaaaaatt gcccactagt ctgaaacaga aagtcttcga agacctgttt
     1741 gagaacaaat caaaagtgac ggggaaaaac ctcttggaat atctgcagat tcaggataag
     1801 gacatccaga tcgacgactt gagtggcttt gataaggact tcaagaccag cctgaagtca
     1861 tatcttgatt tcaagaaaca gatctttgga gaggaaattg aaaaagaatc aatacagaat
     1921 atgatcgagg atataatcaa atggatcact atctacggta atgataaaga aatgttgaag
     1981 cgcgtaattc gcgcaaatta cagcaaccaa ttgactgagg aacaaatgaa aaagatcact
     2041 ggtttccaat atagtgggtg gggtaacttc tctaagatgt ttcttaaggg gatctctggt
     2101 agcgatgtgt caactggtga aacctttgac atcatcactg ccatgtggga aaccgataac
     2161 aacctcatgc aaatccttag caagaaattt acgttcatgg ataacgtgga ggacttcaac
     2221 tccggaaaag tgggcaaaat tgacaagatt acttatgact ccaccgtgaa agaaatgttt
     2281 ctttcccctg agaataaaag ggcagtgtgg caaaccatcc aagtggccga ggaaatcaaa
     2341 aaggtgatgg gctgcgaacc aaaaaagatc tttattgaga tggcaagggg tggagaaaag
     2401 gtgaagaagc ggaccaaatc taggaaggcc caactgctcg aactgtatgc tgcgtgcgag
     2461 gaagactgta gagagctgat caaagagatt gaggaccggg atgagcgcga tttcaacagc
     2521 atgaaattgt ttctctacta tacacaattt ggaaagtgca tgtatagcgg ggacgatatt
     2581 gatatcaatg agctcatccg gggaaatagt aagtgggatc gcgaccacat ttacccccaa
     2641 tctaaaatta aagacgactc tattgacaac ctggtgctcg ttaataaaac ctataatgca
     2701 aagaagtcca acgagcttct gtctgaagat attcagaaga agatgcactc tttctggctt
     2761 agtctgctga ataagaaact gattaccaag tctaagtacg acaggctgac taggaaaggg
     2821 gacttcacag acgaggagct tagtgggttt attgctcggc agctcgtaga gacgaggcag
     2881 tctaccaaag ccattgcaga tattttcaaa cagatttaca gtagcgaggt ggtgtacgtg
     2941 aagagttcct tggtcagtga ttttaggaag aaacctctga actaccttaa gagtaggaga
     3001 gtcaacgact atcaccatgc caaggatgca taccttaata ttgtggtcgg taacgtttat
     3061 aacaagaagt ttactagcaa ccctattcaa tggatgaaaa agaatcgcga caccaactat
     3121 agcctcaata aggtttttga gcacgatgtg gtgatcaacg gcgaagtgat atgggaaaaa
     3181 tgtacatatc acgaggacac caatacatat gatggaggaa cgctggatcg gattaggaaa
     3241 atcgtggaac gggacaatat cctgtatact gagtatgcct attgcgagaa aggcgaattg
     3301 ttcaacgcaa ccatacagaa taagaatgga aactcaacag ttagcctgaa gaagggtttg
     3361 gatgtgaaga agtatggcgg ctattttagt gctaatacgt cttatttcag tctcattgaa
     3421 ttcgaagata aaaagggaga tcgcgcacga cacattatcg gcgttcctat ctatatcgca
     3481 aacatgctgg agcactctcc ctcagccttc ctggagtact gtgagcaaaa aggttatcaa
     3541 aatgtgcgga tcctcgtgga gaaaatcaag aagaattctc ttctgattat caacggatat
     3601 cccctgcgca taagaggaga gaacgaagtg gatacgtcat ttaaacgggc gattcagctg
     3661 aaactcgatc agaagaacta cgagctcgtg agaaatattg aaaagttcct cgagaagtac
     3721 gttgaaaaga aaggtaatta tcctatcgat gagaaccgcg atcacattac gcacgagaaa
     3781 atgaatcaac tgtatgaagt actgctctcc aaaatgaaga agttcaataa aaagggcatg
     3841 gccgatccct cagaccgaat tgagaaatcc aagccaaagt ttattaagtt ggaggatctg
     3901 atcgacaaaa tcaacgtgat aaacaaaatg ctgaatttgc tccgctgtga caatgatact
     3961 aaagccgact tgtcacttat tgagcttccc aaaaacgccg gctcatttgt tgtcaaaaag
     4021 aatacgatcg gtaagtccaa gattatcctg gttaaccaga gcgtgacagg gttgtacgag
     4081 aaccggaggg agctg
//
