LOCUS       sequence-195233-        3033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E0XXB7
FEATURES             Location/Qualifiers
     source          1..3033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagctcaa aggctattga ctccctggag cagctcgacc tgttcaaacc ccaagaatat
       61 actcttgggc tggatctggg catcaagtca attggctggg ccatactttc aggcgagagg
      121 attgccaacg caggagttta cctgttcgag actgccgaag agctgaattc tacagggaat
      181 aagctgatct ctaaagctgc agaacgcgga aggaaaagac ggatccgccg catgctggac
      241 agaaaggcgc gaagaggccg ccatatcaga tatctgctgg aacgcgaagg cctgccgaca
      301 gacgaactgg aggaagttgt cgtgcatcaa tccaacagaa ctctctggga cgtcagagcc
      361 gaggcggtgg agcgcaagct gaccaagcag gagctcgccg ccgttctctt ccacctcgtg
      421 agacaccggg gctacttccc caacacgaaa aagctgccac ccgatgacga gagcgattcc
      481 gctgatgagg agcagggcaa gataaaccgg gcgacaagca gacttaggga agagctcaag
      541 gcctcagatt gtaaaacaat cggtcaattt ttggcccaga atcgcgatag gcagaggaac
      601 agagagggag actactctaa cctgatggct cgcaaactgg tgttcgaaga agcgctccag
      661 atactggcat ttcaacgcaa acaggggcac gagctctcaa aggacttcga gaaaacctac
      721 ctcgatgtac ttatgggtca gagaagcggc cgatctccca aactcggcaa ctgcagtctg
      781 ataccttctg aactgagagc cccttcatcc gcccccagca cagaatggtt caagtttctg
      841 caaaacctgg gcaatttgca gatttccaat gcctacagag aggaatggtc cattgacgct
      901 cctagaagag cacagatcat tgacgcatgt tcacagaggt ctaccagcag ttactggcag
      961 attcggaggg acttccagat cccggacgaa taccggttta acttggtgaa ctatgagagg
     1021 cgcgaccccg atgtggatct gcaggagtac cttcagcagc aggagagaaa aaccctcgcc
     1081 aatttccgca actggaaaca actcgagaag attatcggca ctggccaccc tatccagact
     1141 cttgacgaag cagcaagact tattaccctg ataaaggatg atgaaaagct gagcgaccaa
     1201 ctggctgacc tgctgcccga ggctagcgac aaagcaatta ctcagctgtg tgaactggac
     1261 ttcacaaccg cagcaaaaat ctccctcgaa gcaatgtaca gaatcctgcc tcatatgaac
     1321 cagggaatgg gattctttga tgcatgtcag caggagtctc ttccggagat tggggttcca
     1381 ccagctggcg acagggtgcc ccccttcgac gaaatgtaca accccgtggt aaatcgagtg
     1441 cttagccaaa gcagaaagct cataaacgcc gtgattgacg agtatggaat gcccgccaag
     1501 attagagtgg agctggctag agatctgggg aagggccgcg aacttcggga acgaataaaa
     1561 ctggaccagt tggacaagtc taagcagaat gatcagagag ccgaggactt tcgggccgaa
     1621 ttccagcagg cccccagagg ggaccagtca ctgcggtacc ggctgtggaa ggaacagaat
     1681 tgcacctgtc cttactctgg gcggatgatc cctgtgaatt cagtgctgag cgaagatacc
     1741 cagatcgatc atatactgcc tatctcccag agtttcgata atagcttgtc caacaaggta
     1801 ttgtgcttta cagaggaaaa cgcccagaag tcaaacagga caccatttga gtacctggac
     1861 gccgcggact tccagcgact tgaagccatc tccggtaact ggccagaagc gaaacggaat
     1921 aagttgctcc acaagtcctt tggtaaggtg gctgaggagt ggaaatctag agctctcaac
     1981 gacactcgat atctcacatc agccctggca gatcatctgc ggcaccacct gccggactca
     2041 aaaatccaga ccgtgaacgg acggattaca gggtacctgc ggaaacaatg gggcctggag
     2101 aaagaccgag acaagcacac tcaccacgcc gtggacgcca tcgtcgtcgc ctgcaccaca
     2161 cccgccatcg ttcagcaggt gaccctgtac caccaggaca tcaggagata taaaaaactg
     2221 ggcgaaaaga ggcctacacc atggcctgaa acatttcgac aggacgttct tgacgttgaa
     2281 gaagagattt tcataactcg gcagcccaag aaagtgtctg gagggatcca gaccaaagac
     2341 actctcagga aacacaggtc caaaccagac aggcagcgcg tcgcactcac gaaagtgaag
     2401 ctggcggacc tggagcggct cgtcgagaag gatgcttcta atagaaactt gtacgagcac
     2461 ctgaaacagt gcttggagga atcaggggac cagcccacca aagcgttcaa ggcacccttc
     2521 tatatgccct ctggcccgga ggcgaaacag aggccaatcc tgtcaaaagt gacgctcctg
     2581 cgcgagaaac ctgagcctcc taaacaactg accgagttga gcggggggcg aaggtacgat
     2641 tccatggcac aaggccgact cgatatttat cggtataagc ccggaggcaa aaggaaggac
     2701 gagtaccggg tcgtgcttca gagaatgatc gatcttatga ggggggaaga gaatgtacac
     2761 gtgtttcaga aaggcgtgcc ctacgaccaa ggcccagaga tagaacagaa ttatacattc
     2821 ttgttcagcc tgtatttcga cgaccttgtg gagttccaga gaagcgctga cagcgaggtc
     2881 attagagggt attaccggac ctttaacatc gcgaacggac agctgaagat ctctacctac
     2941 ctggagggtc gccaagactt tgactttttt ggggccaacc ggctggcaca ttttgccaaa
     3001 gtccaggtga acctcctcgg gaaggtgatt aaa
//
