LOCUS       sequence-195237-        3276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 coding gene template with optimized sequence for human codon 
            usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, 
            Lee JH, Kwon S, Bang D
  TITLE     High-throughput construction of multiple cas9 gene variants via 
            assembly of high-depth tiled and sequence-verified oligonucleotides.
  JOURNAL   Nucleic Acids Res. 2018 May 18;46(9):e55. doi: 10.1093/nar/gky112.
  PUBMED    29529247
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-05-18"; volume: "46(9):e55. doi"; pages: " 
            10.1093/nar/gky112"
COMMENT     Sequence Label: pUC19-E3HCA8
FEATURES             Location/Qualifiers
     source          1..3276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagtatt ctatcggact ggacattggg atcgcgtcag tgggttggag cgtcatcaac
       61 aaggacaagg agagaataga ggacatgggg gtgagaatct tccagaaagc agagaacccg
      121 aaggatggat ccagccttgc atcctcacgc cgcgaaaaga ggggatctcg gcgccgaaac
      181 cgacggaaaa agcatcggct ggataggatc aagaatattc tctgtgagtc cggccttgtg
      241 aagaaaaatg aaatcgagaa gatttataaa aatgcgtacc tgaaaagccc gtgggagttg
      301 cgcgccaagt ctctcgaggc caagatatcc aacaaagaga tcgctcagat tctgctccac
      361 attgccaaaa ggcggggctt taaatccttt cggaaaaccg accggaacgc tgacgatact
      421 ggtaagctct tgtctggaat acaggagaac aagaaaatta tggaagagaa aggatacctg
      481 accatcggtg atatggtggc gaaggatcca aagtttaata cccacgttcg aaataaggcc
      541 ggctcatacc tgttttcttt tagccgcaaa ctgctggaag acgaggtaag gaagatccag
      601 gccaagcaga aagagctggg aaacacgcac ttcacagacg atgtgctcga aaaatatatt
      661 gaagtcttta atagccagag gaactttgac gaaggaccta gcaaaccctc cccttactac
      721 tcagaaatcg gccagatagc caaaatgata ggcaactgca cctttgaatc ttctgaaaaa
      781 agaaccgcaa aaaatacttg gagcggcgaa cgattcgtgt ttctgcagaa attgaacaac
      841 ttcagaatcg tgggactgag cggtaaaagg cccctgaccg aagaagaacg ggacatagtg
      901 gagaaagagg tgtatctgaa gaaggaggtg cggtatgaga aactgaggaa aatcctctac
      961 ctcaaggagg aggagcgatt cggggacctc aactacagca aagatgaaaa gcaggacaaa
     1021 aaaactgaaa agaccaaatt tatctcattg atcggcaatt ataccatcaa aaaactgaat
     1081 ctctctgaga agcttaagag cgaaatcgag gaagacaaat ctaagttgga taagatcatc
     1141 gagatactca cttttaacaa gtccgacaaa accattgaat ccaatctgaa gaagctcgag
     1201 ctcagcagag aagatattga aattctcctg agcgaggagt tttcaggcac cctgaatttg
     1261 tccctgaagg caattaagaa aattctcccc tatctcgaaa agggcctgtc atacaatgag
     1321 gcgtgcgaga aggccgatta tgattataaa aacaacggga taaagttcaa gcgcggtgag
     1381 ctcctgcccg tcgtcgacaa ggatctgatc gcaaaccctg tcgtgcttag agctatcagt
     1441 caaacaagga aggttgttaa cgccatcatc cggaagtacg gaacaccgca caccattcat
     1501 gtcgaggtag cacgggactt ggcaaaatca tatgacgacc ggcagactat cattaaggaa
     1561 aacaagaagc gggagctgga gaacgaaaaa acaaagaagt ttattagcga agaatttggc
     1621 attaagaatg tgaagggcaa gctcctgttg aaatacagac tgtaccagga acaagagggc
     1681 aggtgtgcct acagtcggaa agagctgagc ttgtctgagg tgattctgga tgagagtatg
     1741 actgatattg atcacattat accctatagc agatccatgg atgactccta ttctaacaaa
     1801 gtcctggtgc tttctggtga aaaccggaag aaaagcaatc tcctgcccaa ggaatatttc
     1861 gacagacagg ggcgcgactg ggataccttc gtccttaacg ttaaggccat gaagattcac
     1921 ccaaggaaaa aatccaatct cctcaaagag aaattcacac gggaagataa caaggactgg
     1981 aaatcacgcg ccctcaacga cactcgatac attagtcgct tcgtggctaa ctacctggag
     2041 aacgcactcg agtacaggga tgatagtcct aaaaagagag tgtttatgat ccccggtcag
     2101 ctgacagccc aactccgcgc cagatggcgc ctgaataagg tacgcgagaa cggcgatctg
     2161 catcatgcac tcgatgcggc tgtggtcgcc gtaaccgacc aaaaagctat caataacatt
     2221 tcaaatattt cccgctacaa ggagctcaag aattgcaagg acgtcatccc ttccatagag
     2281 taccatgccg atgaggagac tggggaggtg tacttcgaag aggtgaaaga tacacgcttc
     2341 ccaatgcctt ggagcgggtt tgaccttgag ctgcagaaga gactggagag cgagaatcct
     2401 cgcgaggagt tttataatct tctttccgac aagaggtatc tcgggtggtt taactacgaa
     2461 gagggattca tcgaaaaact gcgccccgtg tttgtaagta ggatgcccaa cagaggggtg
     2521 aaggggcagg cacatcagga gacaattcgc tctagtaaaa agatctccaa ccagatagct
     2581 gtgagtaaga aaccgctcaa tagcatcaaa ctgaaagatc tggagaaaat gcagggccgg
     2641 gacaccgaca ggaaactcta tgaagccctg aaaaaccgcc tggaggaata cgacgacaaa
     2701 cccgagaaag ccttcgctga accattctat aagcccacca attctggcaa gcgcgggccc
     2761 ctggtccgcg gtataaaggt ggaggaaaag cagaatgttg gcgtgtacgt taatggagga
     2821 caggctagca acggaagcat ggtccgaatt gacgtatttc gcaagaacgg caagttctac
     2881 accgtcccta tatacgtgca ccagaccctg ctcaaggaac tccctaaccg ggctatcaat
     2941 ggcaagccat ataaagactg ggacctgatc gatggctcct tcgagttctt gtatagcttc
     3001 tatcccaatg atctcattga gattgagttt ggaaagtcca aaagcattaa aaacgataat
     3061 aaattgacta aaacagagat acccgaagta aacctgtctg aagtgttggg ttattatcgg
     3121 ggcatggaca caagcaccgg cgccgcaacc atagacactc aagatggcaa gatccagatg
     3181 cgaataggaa ttaagacagt taagaatatt aagaaatacc aagttgatgt actgggaaac
     3241 gtgtataagg tgaagcggga aaaaaggcag accttc
//
